Hi Scott, Galaxy itself requires samtools (to deal with BAM files), separately from any Tool Dependency within the tool calling framework. Right now there is no mechanism for datatype definitions to declare a dependency (e.g. on a binary like samtools, or a Python library). Probably samtools should be listed as a requirement on the wiki? https://wiki.galaxyproject.org/Admin/GetGalaxy Peter On Fri, Feb 26, 2016 at 5:49 PM, Scott Szakonyi <Scott.B.Szakonyi.1@nd.edu> wrote:
Hi,
I'm having trouble uploading BAM files in our development environment. Other files types are uploading without issue. I'm testing with a small BAM file I downloaded, and I'm able to successfully upload it on our production server and usegalaxy.org. When I try to upload in the development environment, I get the following error:
Traceback (most recent call last): File "/vectorbase/web/Galaxy/galaxy/tools/data_source/upload.py", line 431, in <module> __main__() File "/vectorbase/web/Galaxy/galaxy/tools/data_source/upload.py", line 420, in __main__ add_file( dataset, registry, json_file, output_path ) File "/vectorbase/web/Galaxy/galaxy/tools/data_source/upload.py", line 347, in add_file if link_data_only == 'copy_files' and datatype.dataset_content_needs_grooming( output_path ): File "/vectorbase/web/Galaxy/galaxy/lib/galaxy/datatypes/binary.py", line 218, in dataset_content_needs_grooming version = self._get_samtools_version() File "/vectorbase/web/Galaxy/galaxy/lib/galaxy/datatypes/binary.py", line 179, in _get_samtools_version raise Exception(message) Exception: Attempting to use functionality requiring samtools, but it cannot be located on Galaxy's PATH.
I've checked my installed tools, and all the same Samtools packages are successfully installed on the development environment as in production. I don't see any missing dependencies or anything like that. At this point I'm stumped. If anyone can offer some guidance on how to resolve this, I'd be most appreciative.
Best regards,
-- Scott B. Szakonyi Research Programmer
Center for Research Computing 107 Information Technology Center Notre Dame, IN 46556 http://crc.nd.edu
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