Hi Jennifer,
Today I was trying to pull some bowtie2 indices from Galaxy rsync server for PhiX to run some tests and just got the ones for bowtie1… I'm wondering what's the state in regards to this past thread and what we can do to help in here.
Cheers!
Roman
7 mar 2013 kl. 20:01 skrev Jennifer Jackson <jen@bx.psu.edu>:
> Hi Brad (and Roman),
>
> The team has talked about this in detail. There are a few wrinkles with just pulling in indexes - Dan is doing some work that could change this later on, but for now, the rsync will continue to point to the same location as Main's genome data source. This means that there are some limits on what we can do immediately. Setting up a submission pipe is one of them - there just isn't resource to do this right now or a common place distinct from Main to house the data. A few other ideas came up - we can chat later, each had side issues.
>
> But I saw your tweet and think that it is great that you are pulling CloudBioLinux data from the rsync now, so let's get as much data in common as possible, so you have data to work with near term.
>
> I am in the process of adding bt2 indexes - some are published to Main/rsync server already and some are not, but more will show up over the next week or so (along with more genomes and other indexes). I'll take a look at what you have and pull/match what I can. Genome sort order and variants are my concerns, both require special handling in processing and .locs. If it takes longer to check, I am just going to create here if I haven't already. The GATK-sort hg19 canonical is already on my list - it needed all indexes, not just bw2. When the next distribution goes out, I'll list what is new on the rsync in the News Brief.
>
> For the Novoalign indexes, I'm not quite sure what to do about those yet. Or for any indexes associated with tools or genomes not hosted on Main. Do you want to open a card for those and any other cases that are similar? We can discuss a strategy from there, maybe at IUC, if Greg/Dan thinks it is appropriate. Please add me so I can follow.
>
> I'll be in touch as I go through the data. Thanks for your patience on this!
>
> Jen
> Galaxy team
>
> On 2/21/13 12:43 PM, Brad Chapman wrote:
>> Hi all;
>> Is there a way for community members to contribute indexes to the rsync
>> server? This resource is awesome and I'm working on migrating the
>> CloudBioLinux retrieval scripts to use this instead of the custom S3
>> buckets we'd set up previously:
>>
>> https://github.com/chapmanb/cloudbiolinux/blob/master/cloudbio/biodata/galaxy.py
>>
>> It's great to have this as a public shared resource and I'd like to be
>> able to contribute back. From an initial pass, here are the things I'd
>> like to do:
>>
>> - Include bowtie2 indexes for more genomes.
>>
>> - Include novoalign indexes for a number of commonly used genomes.
>>
>> - Clean up hg19 to include a full canonically sorted hg19, with indexes.
>> Broad has a nice version prepped so GATK will be happy with it, and
>> you need to stick with this ordering if you're ever going to use a
>> GATK tool on it. Right now there is a partial hg19canon (without the
>> random/haplotype chromosomes) and the structure is a bit complex.
>>
>> What's the best way to contribute these? Right now I have a lot of the
>> indexes on S3. For instance, the hg19 indexes are here:
>>
>> https://s3.amazonaws.com/biodata/genomes/hg19-bowtie.tar.xz
>> https://s3.amazonaws.com/biodata/genomes/hg19-bowtie2.tar.xz
>> https://s3.amazonaws.com/biodata/genomes/hg19-bwa.tar.xz
>> https://s3.amazonaws.com/biodata/genomes/hg19-novoalign.tar.xz
>> https://s3.amazonaws.com/biodata/genomes/hg19-seq.tar.xz
>> https://s3.amazonaws.com/biodata/genomes/hg19-ucsc.tar.xz
>>
>> I'm happy to format these differently or upload somewhere that would
>> make it easy to include. Thanks again for setting this up, I'm looking
>> forward to working off a shared repository of data,
>> Brad
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>
> --
> Jennifer Hillman-Jackson
> Galaxy Support and Training
> http://galaxyproject.org
>
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