Hello, I have been using the galaxy API to upload files into a library (using a local folder for library import) and running a workflow into a history. I have been following the example scripts that come with galaxy. When I upload a gzipped fastq file, in autodetection mode (linking the file and not copying, to be faster), the file is not detected as fastq. Even if I explicitly say that it is a fastq, the file is uploaded as a fastq but with its contents gzipped (so downstream analysis fail). Nonetheless, if I do this manually through the interface, the file is unzipped correctly. Any idea how can I upload the gzipped fastq through the API so that it can be used properly? Thank you. ===================================== Daniel Sobral Next Generation Sequencing Data Analyst IGC - Instituto Gulbenkian de Ciencias e-mail dsobral@igc.gulbenkian.pt =====================================