Hi, this thread is likely related to this one: http://user.list.galaxyproject.org/Installing-galaxy-with-Apache-td4654499.h... I installed Galaxy on a Ubuntu Server 12.04 system. I followed the instruction on the link mentioned above and this one: https://wiki.galaxyproject.org/Admin/Config/ApacheProxy Now my issue is: When I got this configuration in my /etc/apache2/sites-available/default : RewriteEngine on RewriteRule ^/galaxy$ /galaxy/ [R] RewriteRule ^/galaxy/static/style/(.*) /home/galaxy/galaxy-dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/galaxy/static/scripts/(.*) /home/galaxy/galaxy-dist/static/scripts/packed/$1 [L] RewriteRule ^/galaxy/static/(.*) /home/galaxy/galaxy-dist/static/$1 [L] RewriteRule ^/galaxy/favicon.ico /home/galaxy/galaxy-dist/static/favicon.ico [L] RewriteRule ^/galaxy/robots.txt /home/galaxy/galaxy-dist/static/robots.txt [L] RewriteRule ^(.*) http://localhost:8080$1 [P] Galaxy is visible at www.my_server_url.de<http://www.my_server_url.de> but not at www.my_server_url.de/galaxy<http://www.my_server_url.de/galaxy> Following the later URL I got this message: Proxy Error The proxy server received an invalid response from an upstream server. The proxy server could not handle the request GET /galaxy<http://www.gsa.npz.de/galaxy>. Reason: DNS lookup failure for: localhost:8080http: ________________________________ Apache/2.2.22 (Ubuntu) Server at www.my_server_url.de Port 80 When I change the configuration like this: (make the second line a comment): RewriteEngine on #RewriteRule ^/galaxy$ /galaxy/ [R] RewriteRule ^/galaxy/static/style/(.*) /home/galaxy/galaxy-dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/galaxy/static/scripts/(.*) /home/galaxy/galaxy-dist/static/scripts/packed/$1 [L] RewriteRule ^/galaxy/static/(.*) /home/galaxy/galaxy-dist/static/$1 [L] RewriteRule ^/galaxy/favicon.ico /home/galaxy/galaxy-dist/static/favicon.ico [L] RewriteRule ^/galaxy/robots.txt /home/galaxy/galaxy-dist/static/robots.txt [L] RewriteRule ^(.*) http://localhost:8080$1 [P] Galaxy is visible both at www.my_server_url.de<http://www.my_server_url.de> and at www.my_server_url.de/galaxy<http://www.my_server_url.de/galaxy> When I completely remove this part, the "It works!" standard page of apache is displayed. The configuration in my universe.ini is as follows: host = 127.0.0.1 [filter:proxy-prefix] use = egg:PasteDeploy#prefix prefix = /galaxy [app:main] filter-with = proxy-prefix cookie_path = /galaxy Can you please help me with this issue? I want to see the "It works!" page at www.my_server_url.de<http://www.my_server_url.de> and galaxy only at www.my_server_urla.de/galaxy. Thanks in advance! Kind regards Matthias Enders