FTP is needed for files over 2 GB by default. Try FTP upload. Please contact your campus administrator if FTP is not enabled. Your admin is the best resource for alternatives if FTP is not active and will not be (admin loads data into libraries, and the like).


Please note that the target FTP server is usually the same as the instance's base URL and the credentials (email/pass) are the same as for your Galaxy account on that instance. This information is unique for every Galaxy instance. Try using Filezilla if you are not comfortable with FTP on the command line.

Take care,


Jennifer Hillman-Jackson
Galaxy Application Support

On Wed, Mar 23, 2016 at 4:28 PM, Tiara Smith <tiara.y.smith@gmail.com> wrote:
Hell I am accessing galaxy from a main computer on my university campus. I am having difficulty uploading my exome files and keep getting the following error prompt:

2.8 GB
Failed: File exceeds 2GB. Please use a FTP client.

Is there a way to increase my file upload limit? my data sets range from 1.9GB ro 3.9GB. 

thank you for your help.

Tiara Smith

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