Can I put in a similar question on top of this: How much resources do you need for re-sequencing of a mammalian genome (assembly and variant detection), one job at a time? E.g. how much RAM etc. if I want the re-sequencing SAM file of a 30-fold coverage be done in 48 hours? Gerald Gerald Bothe 32 Plum Hill Road East Lyme, CT 06333 (860) 451 8776
________________________________ From: Nikos Sidiropoulos <nikos.sidiro@gmail.com> To: Peter Cock <p.j.a.cock@googlemail.com> Cc: "<galaxy-dev@bx.psu.edu>" <galaxy-dev@bx.psu.edu> Sent: Wednesday, September 11, 2013 8:19 AM Subject: Re: [galaxy-dev] Scaling and hardware requirements
Hi Peter
It's going to be one big machine, running both Galaxy server and the jobs. It's going to be a multi-process configuration. If that idea is terribly bad please let me know so I can give back the feedback.
De novo assembly can also be for the human/mouse genome.
Bests, Nikos
2013/9/11 Peter Cock <p.j.a.cock@googlemail.com>
On Wed, Sep 11, 2013 at 1:03 PM, Nikos Sidiropoulos
<nikos.sidiro@gmail.com> wrote:
Hi all,
I have a couple of questions regarding a server setup dedicated on Galaxy.
The idea is to buy a 64 core 256GB RAM server. From my experience I believe that Galaxy will be able to scale up to 64 cpu's but I would like some more feedback on this. Also, is 4GB RAM per CPU core enough for NGS data? (including de-novo assembly)
Bests, Nikos
Hi Nikos,
Is this going to be one server both for running Galaxy (which needs fairly low resources) and running jobs for Galaxy, like de novo assemblies (which need high resources)?
i.e. You have one big machine only, no cluster?
For de novo assembly the RAM per core/CPU isn't important, it is the total RAM on the machine. How much RAM you need depends on which assembler you use, the organism (both size and also complexity) and the volume of data.
What you've described should be fine for bacterial assemblies and smaller eukaryotes - beyond that you'll need to give more details.
Peter
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