I am using GATK for first time. I am doing Targeted reseq Analysis. while i am running Unified Genotyper in Galaxy interface. it gives error message as mentioned below..

"ERROR MESSAGE: The fasta file you specified (/tmp/tmp-gatk-WBrGTf) does not exist."

But i places the Fasta file path in .loc file(gatk_sorted_picard_index.loc).

Also could anyone tell what are the preprocessing steps before using Bam file for Unified Genotyper??

Your answers are very much Appericiated.

Thanks

Sridhar