Hi Andrew,

Is this on a local instance or the public Main Galaxy instance at usegalaxy.org (https://main.g2.bx.psu.edu)?

Also, the exact tools being used and the order is not clear. Maybe a step is missing from what is listed? (I am just guessing) If you are using the public Main instance, you can share a history with me and I can provide feedback. If this is on your instance, could you list the tools by name, in the order used, with key parameters (e.g. "Generate pileup from BAM dataset" with option 10-column pileup)?

It sounds like there might be a mix-up about the file formats being used as inputs to tools. It may be something that can be resolved by reassigning the datatype or by adjusting the tool(s) used.

Best,

Jen
Galaxy team

On 6/24/13 1:10 PM, Andrew Guo wrote:

After using tools to convert a SAM to a BAM file, I am trying to filter the BAM pileup. However, the filter BAM tool is not picking up on the dataset that I just created. It should show up under 10 column pileup, but an error message is saying "History does not include a dataset of the required format/build". Could you provide any insight into this?

Thanks,

Andrew
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