Dear galaxy-devs, What would be the galaxy way to convert tabular data (e.g. the tabular output of BLAST or infernal) into bed or alignments? For bed it seems like that cutting the columns and using awk to adapt the positions to the bed definitions and switch coordinates for the reverse strand matches would do the job. But, in particular using awk seems to be "end user incompatible" :) What would be the best idea: Provide a workflow or a tool doing the job? Initially I had hoped that I can simply change the data type to bed and assign the columns in the attributes tab (but I forgot about the coordinate adjustments). Anyway there has been a problem: Then the preview in the history shows that the columns are assigned (except for the score column). It looks like this: 1.Chrom 2 3.Name 4 5 6 7 8.Start 9.End 10.Strand 11 12 13 14 15 16 17 18 But in "Show Data" the columns are still taken as is, e.g. 2nd column is start ... When I try to use the File in BedTools Merge it fails with the message that it is not a bed file. Thanks for all the help. Best, Matthias -- ------------------------------------------- Matthias Bernt Bioinformatics Service Molekulare Systembiologie (MOLSYB) Helmholtz-Zentrum für Umweltforschung GmbH - UFZ/ Helmholtz Centre for Environmental Research GmbH - UFZ Permoserstraße 15, 04318 Leipzig, Germany Phone +49 341 235 482296, m.bernt@ufz.de, www.ufz.de Sitz der Gesellschaft/Registered Office: Leipzig Registergericht/Registration Office: Amtsgericht Leipzig Handelsregister Nr./Trade Register Nr.: B 4703 Vorsitzender des Aufsichtsrats/Chairman of the Supervisory Board: MinDirig Wilfried Kraus Wissenschaftlicher Geschäftsführer/Scientific Managing Director: Prof. Dr. Dr. h.c. Georg Teutsch Administrative Geschäftsführerin/ Administrative Managing Director: Prof. Dr. Heike Graßmann -------------------------------------------
Hi Rui, Are you trying to set up proftpd (with sftp) to use galaxy user credentials read from a postgresql database? Do you have the postgresql part working on regular ftp? 1- The proftpd version on ubuntu 16.04's repository works fine, you don't need to compile it yourself (unless you really want/have to). An apt install proftpd-basic proftpd-mod-pgsql would do it. 2- The user and group under which proftpd runs are defined in proftpd.conf. You didn't show that part of your file, but it's these two lines: User proftpd Group nogroup 3- I see you're missing: #Before the <IfModule mod_sftp.c> LoadModule mod_sql_postgres.c LoadModule mod_sql_passwd.c AuthPAM off #Within the <IfModule mod_sftp.c> TLSProtocol TLSv1 I suppose you can specify tls 1.1 or 1.2 here, but never really tried it. In my setup I also commented out the AuthOrder line. I guess these changes should do it. Best, Tiago On 16-03-2017 15:58, Matthias Bernt wrote:
Dear galaxy-devs,
What would be the galaxy way to convert tabular data (e.g. the tabular output of BLAST or infernal) into bed or alignments?
For bed it seems like that cutting the columns and using awk to adapt the positions to the bed definitions and switch coordinates for the reverse strand matches would do the job.
But, in particular using awk seems to be "end user incompatible" :)
What would be the best idea: Provide a workflow or a tool doing the job?
Initially I had hoped that I can simply change the data type to bed and assign the columns in the attributes tab (but I forgot about the coordinate adjustments).
Anyway there has been a problem: Then the preview in the history shows that the columns are assigned (except for the score column). It looks like this:
1.Chrom 2 3.Name 4 5 6 7 8.Start 9.End 10.Strand 11 12 13 14 15 16 17 18
But in "Show Data" the columns are still taken as is, e.g. 2nd column is start ...
When I try to use the File in BedTools Merge it fails with the message that it is not a bed file.
Thanks for all the help.
Best, Matthias
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participants (2)
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Matthias Bernt
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Tiago Macedo