Hi Jan, Since this question is about a Cloud installation, I am going to forward your question over to the galaxy-dev@bx.psu.edu mailing list so that the development community will have a better chance of seeing it and providing feedback. http://wiki.g2.bx.psu.edu/Support#Mailing_Lists Thanks! Jen Galaxy team On 3/6/12 1:44 PM, Jan R McDowell wrote:

Hi all, I have been trying to get an instance of Galaxy going on the EC2. I have no problem going through BioCloudCentral and getting an instance going. I can also successfully load my data from an S2 bucket into Galaxy. The problem occurs when I try to use velveth. I always says 'job waiting to run'. As a matter of curiosity, I then used SSH to get into CloudBioLinux, which worked. However, when I try to use NX to get the virtual desktop going I get the message usr/bin/nxserver: line 381: echo: write error: No space left on device.

Using the df -h command, I get:

Filesystem Size Used Avail Use% Mounted on /dev/xvda1 20G 19G 0 100% / udev 8.4G 4.0K 8.4G 1% /dev tmpfs 3.4G 660K 3.4G 1% /run none 5.0M 0 5.0M 0% /run/lock none 8.4G 0 8.4G 0% /run/shm /dev/xvdb 404G 202M 383G 1% /mnt /dev/xvdg1 700G 654G 47G 94% /mnt/galaxyIndices /dev/xvdg2 10G 1.7G 8.4G 17% /mnt/galaxyTools /dev/xvdg3 200G 11G 190G 6% /mnt/galaxyData

So, I guess my question as a new user is: How do I point Galaxy and CloudBioLinux to all of this unused space? I assume the problem is with the /dev/xvda1 that is 100% full. I am obviously doing something silly and/or missing a really big step. Any help would be greatly appreciated.

Many thanks in advance, Jan

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