Hi all, My apologies for reposting this, but I thought I ought have asked this in a separate thread instead of appending it to a previous message. Anyway, I was able to set up a folder for a user (in my case, my own non-admin user account). universe_wsgi.ini's been configured to allow users to utilize a data library. I've also created a library for my non-admin user account. When all was set, I tried uploading a file to the library. But Galaxy prompted me that the folder did not contain any valid files. I thought I provided the wrong path; I even thought I really did not put anything in the folder. But when I checked, every thing seemed fine. I'm really stumped. I've searched the mailing list and even the wiki, and unfortunately I was not able to find a decent answer. I changed the file extension to .fastq and I indicated that the file to be uploaded was a FASTQ one, as Jen has suggested. I still got the same error in the end. I've scanned the universe_wsgi.ini file to see if there's anything else that I needed to configure, but I found none. Thanks in advance for your help! CL