Dear developers, Since last Galaxy upgrade some issue with uwsgi and threading arise. I used Galaxy documentation<https://docs.galaxyproject.org/en/master/admin/scaling.html> as a guideline to configure galaxy.yml: uwsgi: socket: 127.0.0.1:4001 http: 0.0.0.0:8080 buffer-size: 16384 processes: 4 threads: 8 offload-threads: 2 static-map: /static/style=static/style/blue static-map: /static=static master: true virtualenv: .venv pythonpath: lib module: galaxy.webapps.galaxy.buildapp:uwsgi_app() thunder-lock: true die-on-term: true hook-master-start: unix_signal:2 gracefully_kill_them_all hook-master-start: unix_signal:15 gracefully_kill_them_all py-call-osafterfork: true enable-threads: true mule: lib/galaxy/main.py mule: lib/galaxy/main.py mule: lib/galaxy/main.py farm: job-handlers:1,2,3 When I run Galaxy, I got error message - error: can't start new thread Also if I run some job which require a lot of threads, I always get error message - RuntimeError: can't start new thread I tried to increase number of processes in /etc/security/limits.conf for galaxy user but it didn’t help. What can be solution? Do you have any idea what could be wrong? Thanks a lot for your support. Best, Marija Marija Đurđević PhD, Diagnostic & Research Center for Molecular BioMedicine Institute of Pathology Bioinformatician, Core Facility Computational Bioanalytics Center for Medical Research Medical University of Graz Stiftingtalstraße 24, A-8010 Graz Austria Phone: +43 316/385-73024 Fax:+43 316/385-73009 Email: marija.durdevic@medunigraz.at<mailto:marija.durdevic@medunigraz.at> Email: marija.djurdjevic@klinikum-graz.at<mailto:marija.djurdjevic@klinikum-graz.at> Web: https://zmf.medunigraz.at<https://zmf.medunigraz.at/>