Hi Zain, The minimum requirements for Galaxy are pretty much any recent model computer. A basic install will load and run within a very short amount of time, following the instructions at: http://getgalaxy.org Advanced tools come from the Tool Shed. These are installed separately. No programming is needed, but some very basic unix skills are required, as they would be for ongoing server maintenance. The Tool Shed is one of the best documented parts of Galaxy, so you should be able to find all the answers here, or this is the right list to use for questions about local installs (galaxy-dev@bx.psu.edu): http://wiki.galaxyproject.org/Tool%20Shed However, running a production instance that is intended to run compute intensive tools (such as Tophat2), and where you have some throughput goals, will require more substantial resources. This is always a difficult question to answer since so much depends on the tools used and the data volume. But in general, minimum requirements are about around the same as what those underlying tools would require on their own, if run line-command. So for Tophat or Tophat2, and really the entire Tuxedo RNA-seq tool package, you might be able to get by on 8G memory and 2 or 4 cores. But, it will probably be slow and if you are running replicates through Cuffdiff at the end, you might run out of memory if the files are large and the genome is large (such as human). And if you are are hosting a web Galaxy at the same time with visualizations and such, well, this is why systems are often set up with clusters. With all of these will be competing for the same resources, going low can work, but this will be something that will have to be managed/tested, and it will change through time as tools upgrade. You could test this out by setting up a cloud instance with the hardware you plan to use, loading some of your data, and running your workflow to see how this benchmarks. Have users on the instance while you are doing this - to judge performance. Cloud installations come with many of the advanced tools and data indexes already installed/configured, so this would be less investment than buying the hardware first and finding out later it was not not enough. http://usegalaxy.org/cloud And of course Slipstream Galaxy is an option. The whole intention here is to make a complete package with tools & data already configured, in a system that has enough compute capability to do the work, for scientists/labs who do not want to deal with administrative tasks or work in the cloud (for whatever reason). http://bioteam.net/slipstream/galaxy-edition/ Good luck with your decision! Other are welcomed to comment about how they have set up their system. Jen Galaxy team On 7/15/13 7:14 AM, Zain A Alvi wrote:

Hi Jen,

I hope this reaches you well. I have a small question in regards to setting up a galaxy server. My mentors and I are looking into buying a server for doing NGS analysis through the use of Galaxy. We saw that slipstream's specifications for hardware to be the following:

CPU: 2x Intel Xeon Processor E5-2690, 8 core (16 cores total) RAM Memory: 12x 32GB RDIMM (384 GB) with option to upgrade it to 512 GB Storage (Hard Drive space): 7x 3TB SAS 6 Gbps (16 TB usable) with 1 x 100GB Solid State Disk Power: Dual Pedundant Power Supplies Network: Dual Gigabit Network Adapter

We are wondering what can be the minimum server hardware specifications that Galaxy be run on.

Our second question is if we install Galaxy on the server, do all the tools currently available on Galaxy come pre-installed on it or do we have to program (Via Perl and Python) and install each of those tool sets ourselves. If we have to install those tools ourselves, is there a guide that we can do so? Lastly, how can we upgrade the tool sets such as Tophat 1.44 to Tophat 2 on this server. I was wondering about the last question as Tophat 1.44 is available on the main Galaxy server whereas Tophat 2 is available on the test Galaxy server.

Sorry for so many questions. Thank you again for all the great help.

Sincerely,

Zain

-- Jennifer Hillman-Jackson Galaxy Support and Training http://galaxyproject.org