Hi James, The FASTX fastq to fasta conversion tool requires your input to be in one of the "fastqsanger,fastqsolexa,fastqillumina" datatypes. Please note that this does not include the base "fastq" datatype. Can you double check the datatype assigned to your datasets? Alternatively, there is also another FASTQ to FASTA converter available which does not have this restriction: https://usegalaxy.org/tool_runner?tool_id=fastq_to_fasta_python Thanks for using Galaxy, Dan On Oct 10, 2013, at 6:54 AM, James Tarver wrote:

So I have used Galaxy to convert >50 different FASTQ files into FASTA with no problems before over the past year. The sequencer and FASTQ file format have been identical, however, yesterday when I tired to use the the FASTQ to FASTA converter I couldn't get it to recognise my files.

The drop down 'FASTQ Library to convert:' tab was empty and unable to select the dataset to convert

I then switched histories to one where this action had already been done to repeat it and make sure it was nothing odd with the file I uploaded. However, again the tab was empty and I was unable to select the file to convert.

Suggestions?

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