Dear Developers, I am GATK (beta ) Unified Genotyper module to get the indels and SNP. I placed the jar file in (/galaxy-dist/tool-data/shared/jars/gatk) and i link the reference genome in .loc file (gatk_sorted_picard_index.loc). I could see the Name i mentioned in the .loc file is appearing in the galaxy page while choosing the Reference genome. Now while running GATK in galaxy , The command runs for a while and gives ERROR as i am getting ERROR as *(The fasta file you specified (/tmp/tmp-gatk-Jjg9Mi) does not exist.)* Could anybody suggest on the same.. Thanks Sridhar