I partially answered my own questions by reading the Tool Dependencies wiki page. I installed velvet to my workstation. When I open the shell to start galaxy, I modify $PATH to include the velvet directory. Now the error I get in Velvet suggests it found the program, but something else is wrong. My new error is: No reads found The reads are fastq files that appear in my history and are data libraries housed in an external harddrive. I have used these same data before on the public server. Please advise, Thanks, Greg From: Thyssen, Gregory - ARS Sent: Tuesday, February 05, 2013 10:38 AM To: 'galaxy-dev@bx.psu.edu' Subject: Velvet program files So I am setting up my local Galaxy on a workstation for my own use. I have installed several programs from the Assembly Main Tool Shed. None works. Are these just the wrappers? Do I need to install, say, Velvet somewhere outside of Galaxy? How do I do this? My errors: 6: velveth on data 2 error An error occurred running this job:Unable to run velveth: No such file or directory 7: Abyss on data 5 error An error occurred running this job: ERROR: Expected exactly 3 arguments; got: 67 /media/My Passport/IM-Data2Bio-10262012/IM-mut1/Im-mutant-bulk_1.fastq /home/galaxy/galaxy-dist/database/files/000/dataset_9.dat 8: MIRA contigs (FASTA) error An error occurred running this job: Traceback (most recent call last): File "/home/galaxy/shed_tools/toolshed.g2.bx.psu.edu/repos/peterjc/mira_assembler/3e7eca1f5d04/mira_assembler/tools/sr_assembly/mira.py", line 33, in mira_ver = get_version() File "/home/galaxy/shed_tool 15: Singleton reads reads for velvet error An error occurred running this job: Error running preppereads.sh /bin/sh: /users/galaxy/galaxyscripts/prepare_pe_reads_for_velvet.sh: No such file or directory Thanks, Greg This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately.