details: http://www.bx.psu.edu/hg/galaxy/rev/61dd78cafa09 changeset: 2517:61dd78cafa09 user: Kelly Vincent <kpvincent@bx.psu.edu> date: Fri Jul 31 11:48:37 2009 -0400 description: Added solid_to_fastq and fastq_conversions converters to Convert Formats section 14 file(s) affected in this change: test-data/s2fq_out1.tabular test-data/s2fq_out2.tabular test-data/s2fq_out3.tabular test-data/s2fq_paired_F3.csfasta test-data/s2fq_paired_F3_QV.qualsolid test-data/s2fq_paired_R3.csfasta test-data/s2fq_paired_R3_QV.qualsolid test-data/s2fq_phiX.csfasta test-data/s2fq_phiX.qualsolid tool_conf.xml.sample tools/next_gen_conversion/fastq_conversions.py tools/next_gen_conversion/fastq_conversions.xml tools/next_gen_conversion/solid_to_fastq.py tools/next_gen_conversion/solid_to_fastq.xml diffs (2324 lines): diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_out1.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_out1.tabular Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,576 @@ +@1831_573_1004/1 +AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG ++ +><C&&9952+C>5<.?<79,=42<292:<(9/-7 +@1831_573_1050/1 +TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT ++ +;@@17?@=>7??@A8?==@4A?A4)&+.'&+'1, +@1831_573_1067/1 +AAGTATGAGTCATTTACCGGGATGCGAAGAAAAG ++ +++#%(',%/$,+&1#&),)&+'*'*%#$&#%('$ +@1831_573_1219/1 +CGCCCTATAATAATACAGCGCGTTAGACCGCTCA ++ +@@=4/+)5)408?'665>*/5?<61';<3,:,5- +@1831_573_1242/1 +GCTGAATCGCACCTAGCAACTATAGCCGGGCCGC ++ +>>::>17=A5?@@=;7A=;2.60>82<8=74+;; +@1831_573_1333/1 +AGAATCGTTACCACACACTGCGTCTGGGTATCCG ++ +='@#%3=.>)/34*117,,/6-4+.9742456<) +@1831_573_1362/1 +CGATCTCAACCAGGTCCGCGCCCACCCCTGCCTC ++ +B@@?@@?@C@BA?@>@<@8A@?@'.8'?17:,+? +@1831_573_1448/1 +TCACGCCGGTCCTTGACTGGCGTTCTCTTCGAGG ++ +7=A)?,.@A@@:@@<=/7)@<-#8662%9613&+ +@1831_573_1490/1 +CTCGTCATGTTACGCAAAGGCACGTCACAGCACC ++ +@@@?,@??@$><=>+%/*>*1,)?26&12'9%7. +@1831_573_1523/1 +ATGGAACGGAACGGGTGAGGAGGGGAACGTAGGG ++ +,//*,#8''.6''6'2&27/80)%�'%#*,2' +@1831_573_1578/1 +CGAGTAGCAAACAAGACGCCAATCCAGGCGACCC ++ +?=@<@C@?@@??=>@;7<<8<694)3511(+1<. +@1831_573_1647/1 +AGGGGTTTACACTATTCGACTGGGTGAGAGGCGT ++ +?@@<@@???;A==@@;9?@?3=;6.4/;6:=/;4 +@1831_573_1684/1 +TTCAACTGCGTCGACGTAGCGCACAGGCGTCCGT ++ +>1:8<@39@@??9=;@@<>?@@<;<?7>76?9>? +@1831_573_1769/1 +TGGACGTATAGTGGCGATGAGCATGTAGGTTCTC ++ +54749&&767%/7;3$-7;3#,3//#,45/#&06 +@1831_573_1853/1 +CAAAACGCCCGGGGCCTCACATGCGCGGCAGTTC ++ +4./B@@72B+/4?@?7?+@9/+99.')2<2&)2& +@1831_573_1943/1 +ATAACGTATGGCAGTGAACGGGCGGAACCTGCCC ++ +>/*1,),@-)'0*>5'$/?6@/.&?8/(')A43, +@1831_573_1977/1 +GGCGTAGGGCTCATTGTGCAAGTATCCGACCTCC ++ +@?6@8@@9A@=4633A7?9?<3:+7>'@%@?%32 +@1831_574_109/1 +TCGGTTGCGTTACTTCATGGGAGGGCTTTACATT ++ +7651#4817>@65<2:84716=788<1995.7?6 +@1831_574_148/1 +CGAACCTCGTATAACGGAGTAGTCGGAAACAGTC ++ +;->3;;3>//>0;;7=,=4-4/+>029<9<624; +@1831_574_185/1 +CCGTTTTGCCTAGTAATGCTCGGCGCAGCGTCGC ++ +<4;%%@7>#*>97%6;+8$#<07%1372$%<54# +@1831_574_243/1 +AGGCACCGTAACTCAGGACATTCTCGATTAGTTA ++ +@@@95@=9?*><45$<@<4%@9?7#=?>7&>?97 +@1831_574_257/1 +ATACCTTCCAAAGCAATAGAATAAAAAACAGTAC ++ +>>B>)@?>B@B?@1%*%,#+00.'(+&5&%#$3& +@1831_574_293/1 +TGCTGCAAATAAACATACAGCCTTCTAATGACTA ++ +')7'.*%#&$'/.$##$.$&*)+*$#8%&%#&&% +@1831_574_389/1 +CATGGCTATGCACCGGTTTGTAGCGTACTCGAGA ++ +)$>/>8):2@:213;;.1)@3%64%+)<7)+$92 +@1831_574_575/1 +TTCTTGGCAAGCGCAGATTATGCGTTCCGCCTAG ++ +?@>=?@>8@@@=@6*%&<='5@<<',0<=4*5/< +@1831_574_592/1 +TCATTTACCACGTCAGGGTCGGAGTCATTCATTA ++ +3-5)19,,<@>4067<2.-864346;9<1/;212 +@1831_574_617/1 +AAGCATCGGCGGGAGCGCAAGCTGGGAAGGTGCC ++ +>@?;>?=@==@>@=5?@+@:@40@><:0)')64- +@1831_574_725/1 +GACAAGATGGCTATTATTTACAATCCGATCTGCA ++ +'>?52+5=;4$6;<8.1<47*9+?7(+5;-).)4 +@1831_574_734/1 +CCTGTACGAAAGAACGTAGGCATGGGCTGGGGGG ++ +-&.$2&<*(-%):/)%5:)/%7+,9034*A.?70 +@1831_574_824/1 +AGCGCAAATTATGCGTTCCGCCTAGCGGAGAACT ++ +@@@;;),+@<-<7;<&89??&29?<$2:A<);8? +@1831_574_959/1 +CGCGCTAGGACTCGGCCCCAAGAGACGTTCCGCC ++ +A=;+?@9;/<@;<8#&&/*%(%.0$0$2)+1$-1 +@1831_574_1062/1 +ACCGGTAATATAAGGCAACATGATTACGGCCACG ++ +@821<//:136/8<1/5.427.;>288/0<1$)/ +@1831_574_1092/1 +GACTGGCGAAATCATCGCGGAAAAACCCCTATCA ++ +8?.,98)03%#,,,(,++$'69'#9'7#';6.%2 +@1831_574_1103/1 +ATCTCCTGATTAGACATATCTCCGTAGCTCACGC ++ +??>?@=>@@A<>?=@==<:?<;58'&&):+35'1 +@1831_574_1116/1 +CACCTCACGTGAGTATAGCAGCCCGAGCGTCACC ++ +@++,)/)?./)%<)2>.==:8?&;44&/&,)/*4 +@1831_574_1194/1 +TTATCACATTTGGGGATCGGAAGGGACTACTTCG ++ +5>;,??9@9@?@1@@=@3=@>@4?@@94?75/,6 +@1831_574_1204/1 +CTTACTGGTCTGCTGGACATATAGTGGCGATGAA ++ +A5A5B@5:;@@,.9?A1?#.?;3),>82%)='7* +@1831_574_1306/1 +ATTGCTTAGATCCAGTGGCGCTCAATACAACGGA ++ +2.7&%''.$&#'%##$#'#%')')$'$%$,)'.1 +@1831_574_1387/1 +GTACTTCTCAATGCTGCACTACTATGTACGCCCC ++ +?A??:@?@@9?@@?9C;:>&).,,);&'<&7(/' +@1831_574_1431/1 +GACCAGTTTCAGGGCTAACCGTCCCTACTCGACC ++ +7?>9BA897;+8*=6#6.+5&-#26$0.,5&'$1 +@1831_574_1560/1 +GGCGTCTTAGGATTGAAGAGGGCCTCCCACCCCC ++ +))&65<4=>99@:597(1&;+&(%&4#,#&&&*$ +@1831_574_1591/1 +TGAGCACTTATGGCTAGGCGTAGGGGACCGTGAG ++ +=<A><?@78@<=;@&@6>8:?,><&&<7>8(8+9 +@1831_574_1624/1 +ACGGGAAGGGCTGGAATCTACCCAGTAGGCATTG ++ +<6.&(,7-+))7..'/;43.)927.(<85&13-) +@1831_574_1826/1 +TACGTCGCGACCGAGCGTTATATAGTCTGACCCC ++ +@<<,))459<?.+57'-'&6?1)(;1;0&%4,#$ +@1831_574_1903/1 +AGTGCAACATCTGCTTTGCTTATCAGCACACGGC ++ +956?@>;7@@@8:@>8><=9>9>@2=71?1:6;/ +@1831_574_1961/1 +GTTTCACTTCGGTTATTAAGAAACCCAAATGGAA ++ +>-07-@>(2'@<#064@%5%@@6.&<<(9)=:47 +@1831_575_54/1 +TTTCTTATGGGTAGAACAGCTGCCACTGACTGAA ++ +=@@68<2;<8;?@<>+9>7,,6972&57:4&9<8 +@1831_575_80/1 +TCTTTGGGTTTGGGGCAATTTGGTATGTTCGTCT ++ +?5><@?/=5%(&.5)+#/0'/'>#$/'37#()6& +@1831_575_192/1 +AACTACGCCCCTTAATTACACAGCGCGTTAGACC ++ +:8<,9(.=/)#%8$,4'<,:)3/.'.5<,>-;7' +@1831_575_197/1 +TTCGCCTACACTTAGATACCTCTTAAACTCAATG ++ +-:?463@A:88?6?437<=.2&1.;@67.4@%2, +@1831_575_223/1 +ACGCACAAAGGAGCTCGGCGCATAGCAACGCAGA ++ +?>?8?@:?;>+$'%&,+#$.--#%$1'&#-7$&' +@1831_575_420/1 +CCCACATGGAAAACACTCACCGCCGAACAGAGCG ++ +2?.>?(-''2+..@),$%1@;')1)?+)7.').. +@1831_575_434/1 +ATCGCTGCGAGGTCACCCTGGTTACGCCCCTTCC ++ +@@><@B@>=@@?>><@?;)@?;6.?=4,'';/3) +@1831_575_444/1 +TAGGCGACCGTGAGGACAAGAGCTGTTGCCTTGA ++ +2@@;<.=?@<+=:A<<@?<</>@><(<8>4&5;> +@1831_575_459/1 +CTTATCAGCACACGGTTTACCAGTCCGACTCCAA ++ +@@C@?A@@A@??A@@'6@@B'=;C@'4<=9,@1. +@1831_575_506/1 +CGCAGAACCCGCACGCTTGTGCTCACCACTGTAC ++ +?)2*5@3+.7=?A:8@,/&):@37/<89'+4*)* +@1831_575_569/1 +AGCAGACTGCTGTAACACGGTGTGGTGTAAGGAT ++ +'9-,@29+479%&>9#&08=>7#9>,69/+'*3) +@1831_575_622/1 +CCAAATCCGGCCCAGTAAGTGTCCTGTCGCACCC ++ +A8B=@@?<@?<>>=?96<497)<4/<':4+;74+ +@1831_575_644/1 +CTTGAATGGCGATCTCGTCGAGGAAATACCACTA ++ +<8>.>&,+)8.10':5(/-62&*.=/%(.<&&18 +@1831_575_663/1 +GGCAACTTATCCGCATTGGTCCCACTGGAGCGCA ++ +@@@@A</;A>@6>0792<5966:?;25+:><1,+ +@1831_575_681/1 +TCTCCTGATTAGACATATCACTGTTGAACACAGC ++ +72?.)25;96-14'*.03'#4#++$)/8+%/((& +@1831_575_711/1 +TATGTTCGTCCACGTCAGACGCGCAAAGTTGCGC ++ +%+:2(#'1+.)&*45-#,<A-%4;>+))<;##*& +@1831_575_730/1 +CACACAGGAACCATAGCGTATGTTATTCACCCCC ++ +%8<%-')5,*1&=#)(1*1)->7$.,4'4'&'*& +@1831_575_904/1 +ACCCGCTTAAAGACGTGAATTTTGCCTCCGCGCC ++ +<><.689@@7@<54:4=@>69=:+6-=42;8<:# +@1831_575_938/1 +TCATCAGGGAAGGCTAGGGGTTTACACTATTCGA ++ +:@@:?@;=8;>=;;:=<93<4.;<6:2<9;4)87 +@1831_575_970/1 +TGACTCCTACAGTCTTTATAGTACCGAGGGAGGC ++ +@=@=;@@><@@:9<:=?;>@=?9>>?@:2===<5 +@1831_575_991/1 +TTCGGCGATCCCCCCCACGGCGCGATGCCGCGCA ++ +>>;7???/4<:::,#&*%'$--5-#(7)&&,-)% +@1831_575_1138/1 +TTGAAAGACCTGAACGCGATTTCATGTTTACTGC ++ +=0;%1936+?96?,?5<:2>@6@<:;4>.7;*:1 +@1831_575_1157/1 +TCGCTGTTTAGATTTCGGGAGGGTACTTCAGTGC ++ +B@B>?A?A@?9'?;?&8?@@6;<@=+<:99+965 +@1831_575_1180/1 +GAATTCACGGCAGTGTTATCACCGTTTCCTTCCA ++ +<B>5?':/78?;1;55:997@@>94=7<186->7 +@1831_575_1283/1 +GGTGGAATACTAAGGACTAATGTGCTGTCTCTTT ++ +C@A54><?A78@<?2>:#>=?@2>80:'?69.'7 +@1831_575_1302/1 +CGACTATTCGTTTCGTCTAGAACGTGACACTAGC ++ +12>/#2'&-2&%'7+$%*#$/1(&&12/$,2,,* +@1831_575_1310/1 +CTTGCTCTCGAGCTATGCCTCAGGACACGCCCTT ++ +<<>663?99@(@7=11@:@@/7??<@<;>'3-7; +@1831_575_1321/1 +CAACACAGTTGAACGGCGGAGGAGTAAAGATGCG ++ +;B@?@>=A@<A?>@>?6>@5>'4@9?)7@7?/89 +@1831_575_1373/1 +CGCTACCGGTTCCAACGGCTGCCTGACTCGCGGA ++ +@@@>@;=9<@??7@>@:@;@;17?630/7=4,2< +@1831_575_1419/1 +TGGGGAATATAACAGCGTAGCGTTGAACACTAGA ++ +//3->&-.->%11/@&/&7:<,-1=,+2<6'%&< +@1831_575_1436/1 +TGCATCTAGCGCGTATTGCGAGCCTTACGGATTC ++ +2@=;39*3<*4<:>52>>9'7:<='0=>1'136< +@1831_575_1442/1 +TCTGACAAGGTTCCTGCACCTGCGTCTGAGAGGG ++ +>58<?7>+1B6-/9<<:5?>A:1@?539.@4/1> +@1831_575_1454/1 +CCTCCTAACCACGAGCCGAGGGGTCTCTGCCCCT ++ +4=?8=6589=0:A=:?>6<;@/,4+-6+/'.,(, +@1831_575_1500/1 +CACAAAAGGTCCCTACCTGTCTACCCTACATAGC ++ +?@@?A@?8<6<:985=99999==B3<5<<,7?>9 +@1831_575_1535/1 +CTCGACGATATGACCGCCAGCCACTTAACTCCGC ++ +B=@<@@B?:A@@=>@?68@=?@5?7;:6<<;>5< +@1831_575_1724/1 +TCGTAAGTGTTAAGGAGCTGTGTACAAAACAACA ++ +<B.+@),2*.%)))4%2@;7#%(%+$8))85%&5 +@1831_575_1829/1 +CATTTGCTGACCCTGCGTAGTTTAGTCTCACAGC ++ +2(*'%.3$+7)@&%$'3*+*#/#/*+0.=&#)+0 +@1831_575_1898/1 +CTTACCATATCATCTCAACCCATAACAGCACTTA ++ +B>@@&1/))'40)%#8/.%#8$((#;4'$'63,, +@1831_575_1964/1 +GACAGACCATGAGGCTGAAGACTACTAAGTGCGT ++ +>39)@2<2/@+9?2=&)>>@*62=5&2<42.'?+ +@1831_576_32/1 +TACGCAACGATTTATGGCCTTATAATTGAGGCCA ++ +<>?>?=7<2)522;><<@40@>704<>5=23@+& +@1831_576_74/1 +ACATTCTGCAGTGGGACAGAGCGGTACGCCGCAA ++ +=@@?@>(??<B5?@@@9<@><+><;@';>6961? +@1831_576_86/1 +ATGAAAACGCATTAGGACAACCATAATGGCCTCA ++ +811)2:*.++5</:3+43924*))/:,6&29)2/ +@1831_576_89/1 +GCTGTTTGATTTGAGAAGAGGAATTAAGCGCCGA ++ +)69+,'.4=-,>/>(*$#)3030*'(,%)2##$, +@1831_576_266/1 +ATGGGGTCACTCGACCTAATCCCGCGGCTTTGGT ++ +@@?=;<?7=@>9,>@1$&&89$/:>7'3178%&6 +@1831_576_327/1 +GCCGTTCTACTCTAGCTGCAACTTGCGATTGCTA ++ +@?@6@@;<@?><@>==3@:==<3@@>53<9><6= +@1831_576_331/1 +GACGCTTTACTCCGGTAGTACCGTGCCGTTTATA ++ +-2)+(*.*1/;5%.-9&#/1'+($*$##()%/$. +@1831_576_387/1 +ACACGCCATGATCCGATAAGAAGGGAACGTAAGG ++ +8).$5#1#*%.$##.*#$%##-%,+,1#&%.%)) +@1831_576_406/1 +AGGTCTTACAGCACGGGGCTGAGCGCATCTGACC ++ +4<>%%14:*4656)&<251&2+3#&19,6&4>5( +@1831_576_449/1 +CTCGAACCGCGGGGTCCAAAGACTGCTGCAAGGA ++ ++'&'.,,$/+.)$$8&%#+?&,#)-&###7,+#* +@1831_576_519/1 +TACCTGCCTACTACTTGCTCTCGAGCTATGCCTC ++ +=46<97@>2/6?;2<4A881>9121+<1/4.9+7 +@1831_576_603/1 +CAATATGTCTTAGTCGTGACTCGGCAACTTATCC ++ +@46=@C.??<A79@@;-<@@>29B?>55<B7598 +@1831_576_655/1 +GAACAGTCTATAGTGGCGGGAATCTCGTCGTCAG ++ +@AA9@@<6*>@@5/<@>9'=;>7+@?9>/9;+,% +@1831_576_677/1 +TTTACTCAGTTGATACATCACTGTAGCACATAGG ++ +290&/*0#&'&,.2'#&*$&('#-%($*#%$)#% +@1831_576_718/1 +CGTGCCTTTCAGGGTCTTTTCTGGTCTGGTCGCT ++ +4###$0###%(#########,####%####$### +@1831_576_722/1 +CGTATGATGGCGAGTCTTTATAATCCAATCTGAA ++ +*,-,##.)*&(*1%*(%(-2#+)-#.&-#%%$') +@1831_576_754/1 +AGGCGTCCTGCATCGACCGTTCTATCCGCTTAGA ++ +4//#(.$)'',>(
$<,##%((,#5?#0*%1* +@1831_576_815/1 +TAGGCCTGATATGACACGATCACAGTGCAACATC ++ +3>@?9>@?B=>=;>A???=>:25=4.25?6<57. +@1831_576_882/1 +TGTAAGACGGTGAGGTGTAAGGATCAGACCACGG ++ +26?8?@:4>@>96??<<=5'1<>9846=<9<1>8 +@1831_576_898/1 +AGTACTGTCGCGCATTGGGGTCCTGGTCGTTGCT ++ +@@C2?C>?<>@886B?;?.??87=B<8<15??=. +@1831_576_923/1 +CTGGACATGAGAGACTGCACGCGGTACACGTCGG ++ +6.51=;.699<96>;;49<;;11;<@59:9=647 +@1831_576_930/1 +CTGGCATGTACGTCCATGTCAGACGAGCAGAACT ++ +#*0.2&.:((#'14'##-)#%$$2%#$/1&#%/# +@1831_576_1019/1 +GATGCGCGCTGTCATGGCATCGAACCATCGGTCG ++ +<$>$,1,&++&@,.)'+/+#9'69/6'2(+-'9- +@1831_576_1068/1 +AAGAGTGACTCACTTACCGGGATGCGATGGAGCC ++ +>><=4?;@7=??9?;9>5@9?:8@:=5';7;'#4 +@1831_576_1131/1 +AGTTCGGGAAGGGCTGGAATCTACCCAGTAGGCA ++ +04@#@/@,4>9?2+?1571@',>=;(759;*92< +@1831_576_1168/1 +CACTTAACTCCGCTGTCGGAAGCCTTACAAGACA ++ +@CC6@@2?9=>7?;76<;467@;9,0%26'',4$ +@1831_576_1207/1 +CAACCTGACTAAACGGGGATACGCTGGCGCTACA ++ +$>2,(&?4?(,@:<&,@>?$&:8A%%=0.%,597 +@1831_576_1289/1 +TAGCGCAAGTCCAGAATGTTCATAGACTCGCGAT ++ +9<>1B@@;7@@663==28,5':8<<,.=2>>.50 +@1831_576_1329/1 +CCAATAGCAGAGACCTAATAGGAAACGAAAGCAA ++ +>&0,870/A@@/5.;=;:'&@3'&$$%,+#($&2 +@1831_576_1367/1 +GGTCTCATCCGTTCCAATCGGGACTTTGACCAGT ++ +?@@<>@@=@<@<=@>@??9:?<=>=<8;59@787 +@1831_576_1416/1 +TAGCGTTCAACGTCGATCTCATCTTGCCGATGGC ++ +';?..1.<@'.=+6.5?7<0-?7;(%=>56.98@ +@1831_576_1461/1 +GAGGGGCGGCCCGGTTCAAGCAGGTAAGCAACAA ++ +1&74#(#(&##$#,'###%#%%#*#&%%##%%%' +@1831_576_1605/1 +AGTGCAACATCTGCTTTGCTTATCAGCACACGGC ++ +<@C@@>1;@:;<<@@9@???9<3?5-21=4877, +@1831_576_1664/1 +CGCGCACAACTCGCCATGATACGACAAGACCCGA ++ +@?>?@=A:@@>@='=<>=).>=9*8)(7#/++;) +@1831_576_1671/1 +GTCTTTGTAAGCCTGGCCTGGTCAGGTCTGGTCT ++ +@?@5>@@8&8?25#&)&5&&)15&&,&4%&,&6% +@1831_576_1729/1 +CGTTTCGTCTACAACGTGACACTAGCACAGTATA ++ +?@?<?<@>A?/==?>?7>?@8<?@>99;><+0=> +@1831_576_1880/1 +TATGCGCTGTTGAGCTTACAACTCACTAGCGAAT ++ +515/792,:,7/%/05,%$):+#8%2(1754))3 +@1831_576_1982/1 +AATGTCGTCAGACGACTTTGGCGCGAAAACCATA ++ +@@?@,A><@9@=?@9B=8<6@@6@2<A?>.7<+@ +@1831_576_1987/1 +AAGGTCTTCTGTCGGCGCTGGACTGAACACCTGA ++ +##()(#&##)%)/-#%$11#%3>'##&$,#$$'+ +@1831_576_2014/1 +CCGTGACACACAATGCCGGCCCCAGCCTAGCAAT ++ +@@9,B29-5>'?,+?79+/A';'2@'5&/9,6&1 +@1831_576_2028/1 +ACTCGCCGCATCCCCGAGTGACGCTCGAGACCAA ++ +<>9>5'2(&707.8#&&39,0%7/#(#,*%&5*+ +@1831_577_40/1 +CCCCGCGTTACGAACGAGAGAAATCTCTTATAAT ++ +2/4('.')(<*?#$)%&<,/39<(.2,+<=@611 +@1831_577_119/1 +TCCCACAAGCCATTGACATGCTCGCTCTAAACAG ++ +>?C11)-1/)#;#/,850*+.+$$5550+%-.40 +@1831_577_133/1 +TGCTTGTACGGTCTAACGGGGTATGGGTTTCTGG ++ +4@>>?=1?:+>@07@@><>@*<+1@15)96'2$) +@1831_577_255/1 +ATTGAGGCCAAGATAATTGAGGAGAGAGAAGGTG ++ +<=87@>69%**#&#-+$.#&&#+$-+%&%.,%5# +@1831_577_281/1 +TATGTACGTCGCGTACACTCCGGGGCCCGCAAAA ++ +-:B;8@=;>7@>4?=?+659?;5<7?;9@8(>:? +@1831_577_288/1 +CATCCGAGGCTATCAAGGCGTAAGCACTGACCTA ++ +;/79??&=B::298*6.7/+4&21,7,6?.7#'6 +@1831_577_322/1 +GAATGCTGGAGTACATTATGACAAACTATCGGAG ++ +=;>4506;255464-<#7+194&2<?65968)7/ +@1831_577_362/1 +CGATTAGTTACCACTCGTATTCGCACGCCCAGGA ++ +2B?:@?@<?958=,3:,90:&'-99,6<5.($+6 +@1831_577_382/1 +GTCGCGTATTCCCCGATGCTATGTAGACTTGCAA ++ +2957/:1))=76(*24;,3+:<.&.&-=1=2/5* +@1831_577_464/1 +TAGAGGCACCCTAACTCAGGGCTTTCTCGATTAG ++ +=<7/<826)>#.'&4204+5#/041.7*91&756 +@1831_577_488/1 +TGAATAGTTATGGCCAGAATGTCTGCACCGATAC ++ +48.,*>6566<?8=<=<2>6;94>;=9>@8924@ +@1831_577_511/1 +GGTGCTTATCAGTTCTTTCTCGGGACTTGTATTT ++ +7/+&$:<7%6,$$%'%/+)#$7((&*3>16'0/+ +@1831_577_545/1 +ACCGCTCTTTGGGTATGGGGCAATCTGGCATGTT ++ +><<9?<>?A<481@<@8==@76/61<95.5988- +@1831_577_559/1 +GTGCCACTATGTTCGACAGACCCTAAGGCGGAAG ++ +<@;??@>@?/2<.@1=>12=61/;=?.&2+92)' +@1831_577_562/1 +GTTCCACTACGTTCCACGCAAAGGAATCCGAATC ++ +((&*&0%)1%)#($2-,***%/-,,))&,-.1'* +@1831_577_637/1 +GCCTTCGCGGGAGCATATCAGTCGATACATCCCA ++ +-,4#>:-6+:8,&(5;3=0>7=68&1/9&'?;4, +@1831_577_641/1 +TATCTACCACCGCGGTGGCGCGAGAATGCTCCCT ++ +47;/':A;;5?:72,(=),#*?+.#&7$8#%7/' +@1831_577_692/1 +CCGGTGAGAATTACATCGCGAGTACGCCCAAGGA ++ +B,@?@B(@A?@+@@>@+?=>@'=<@<=<9=?75< diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_out2.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_out2.tabular Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,192 @@ +@1378_11_329/2 +GTTCGTGGCCGGTGGGTGTTTGGG ++ +###$$#$#$&#####$'$#$###$ +@1378_17_1788/2 +TGGGTGGATGTGTTGTCGTTCATG ++ +#$#$###$#$#######$#$#### +@1378_25_2035/2 +GTGCGTCGGGGAGGGTGCTGTCGG ++ +######%#$%#$$###($###&&% +@1378_28_770/2 +GGTTGGGAGTTGGCGCGCGTGGTT ++ +###$##%%$####%#%$#%%#($$ +@1378_33_1945/2 +GGGGGGGGGGGGGGGGGGGGGGGG ++ +.6;?;55697>8779457986264 +@1378_34_789/2 +GGGCTTGCGTTAGTGAGAGGTTGT ++ +###%$%$%%###$####$###$#& +@1378_35_263/2 +GGCGGGGGGGGGGGGGGGGGGGGG ++ +$%%&,)3/)%$-6'$&5&#$''5. +@1378_43_186/2 +GCTAGGGTTTGGGTTTGCGGTGGG ++ +$%#$########%##%#$###'#' +@1378_51_1671/2 +GGTGTTGTTCGGATGTAGCGTGGG ++ +##%$$####$########$##0## +@1378_56_324/2 +GCTGGGTGATCTAAGGAATGTAAT ++ +#$#####$$$#$$$$##%###*(# +@1378_56_773/2 +TCTGGTCGGTTTCGGGGAGTGGAA ++ +##%%#&$###$#$##%$####%%$ +@1378_62_2027/2 +GTTGGCCTGGCCTGCCGTGCTGCG ++ +*##),/%##$)#%##1$#'%.#&# +@1378_62_2029/2 +GGCGGTGTGTGGTGCGGCTGTGCG ++ +/$$$=(####%####)$$%$-&%# +@1378_67_1795/2 +CGTGCCAACGACCGTCCTACGGAG ++ +@@.?7@@@.@:@A<<>>=>70<@7 +@1378_68_466/2 +GTGTCATCTGAGGTAAAGCATTGT ++ +/##$09#$#.=$#$76+$%1'### +@1378_68_1692/2 +TGTTCGATTTAGGTCTTGCCTCGC ++ +###%%$%%##%$#$##$###$$#$ +@1378_80_664/2 +TGTCTGCGTTGTATCTCTGGTGTA ++ +%##%,%$$#&$$###$#$%##'%# +@1378_85_1786/2 +CCCTAGGAGCGTATACCGGACGAG ++ +,'&/%/@,&1,&'/)&,6&&1)(( +@1378_86_1011/2 +AGGCGATGGGATATTATTTTACTT ++ +:$###)%##$9$###1$$#$2### +@1378_86_1789/2 +GGCCAGGTACTAATAAATTCAACA ++ +1.51/&%#'$,*?%#6&0$$5,20 +@1378_91_1596/2 +GCTTTTTCATTCGGTGCCTTTGGA ++ +'>%/3%=()8'#.%?50$&5>%)% +@1378_94_1595/2 +GCGGGTTAACCCAGTACCTCTGGC ++ +,8@6'>8<76>@@(->9)7'*941 +@1378_95_1039/2 +GTTCTGTGCCAGGTGAGGTACGGA ++ +&##,./#$&)6##+,'#$$0(##$ +@1378_95_1767/2 +CTAATTGACCGGGCAAGCTATTAA ++ +',,2'(10/>:=/7).3&%6&)&6 +@1378_96_1037/2 +CTGCTGGGCCATTTGACTTACTCA ++ +'$#+#(##-%5##+*&###-.$$$ +@1378_96_1764/2 +TCTTTGTAACCCACTTAGTATTTC ++ +4='28>.@4881B807:822>%)1 +@1378_98_1574/2 +AGGCGAGTGTGGGGGTTGTTTGAG ++ ++%%$#)##%##$####%###$%$# +@1378_107_1647/2 +GGTCTGGTTCTATGTTGGTCGACT ++ +###'$$#$$$(#%###(#$##$%# +@1378_111_829/2 +GGTCTTCTATGGTGCTAAATTAGT ++ +$##'&###$##)#%($#$%#$'%# +@1378_111_1900/2 +GCACGCCTTTGGGCTAAGCCGTAA ++ +)$)'#%$########$'#&%$#(# +@1378_112_1483/2 +TGGAGTGGTGTGTTTGCTGAGCCA ++ +#$#)#############$#%#%'% +@1378_125_1287/2 +TGACGTGGGTTGTCCCGTGAGATT ++ +##$%%#$###$##$$#&%##$(%% +@1378_126_468/2 +AAAAAACGGGCCGGGCGGACTAGG ++ +@@B@@@>@2>1+?:92)>@8&&<+ +@1378_127_664/2 +TCGCTTTGCCTATGTTTGTTCGGA ++ +#%$%#&##$%#%%###$$###)-' +@1378_129_463/2 +AAAAAAAAAAAAAAAAAAAAAAAA ++ +,)&20%8'(&35-***1-2&+510 +@1378_129_875/2 +GACCTTTACGTATTGGGGGTTGGC ++ +###)###+###$##$#&%##$,#$ +@1378_140_1251/2 +TTTCCTTCGTGTGCGTGCGGAGTG ++ +#%#%$##$$$######.$$$%#%( +@1378_141_809/2 +TCTCGTGGTTTCTTTTTTATGTGT ++ +##%)##$$#####%$#$#%%#'## +@1378_144_983/2 +GTTCGTTCGTGGTGTACGAGGGTG ++ +#(#%#####($#%##$$#%##%#) +@1378_153_270/2 +GGCCGTGTGCGGGTGTAGATTGGA ++ +%$##($######&##$&$$$$%## +@1378_155_1689/2 +CGCGTTCGGACAAAGCTAGCACCT ++ +@4@@=6=@@<4:@@681@@@.24. +@1378_157_1580/2 +GGGATTGAAGGGATGTATGCTAGG ++ +#%$&%#$$'%$%#$##*#%$$$$# +@1378_161_317/2 +AATCCATACCCACAAAAGCAGGCC ++ +.&%','(@''?7//+&)+2.+)0) +@1378_177_735/2 +CGAGCCCTAAACCATGAGATCGGA ++ +@@B>@AC9@A<B8@=9>7@5)>,0 +@1378_181_1684/2 +TTTCTGTTGTGGTTTTGTTGGGGT ++ +$##'$%'##%##$%$#$$####$* +@1378_187_1407/2 +TTGGGTGAAATCTTGTCGAGTGGA ++ +####&##$$###$#####%##%%) +@1378_203_721/2 +CCTTTACGATCATAAACCATGACC ++ +9<&./1&@;12')?<1',?/)&/. +@1378_206_2039/2 +CCCCCCCCCCCCCCCCCCCCCCCC ++ +?=>>?=:;<>>4::<=56199(05 diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_out3.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_out3.tabular Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,192 @@ +@1378_11_329/1 +AGACCGGGCGGGGTGGCGTTCGGT ++ +%##+'#######%###$#$##$(# +@1378_17_1788/1 +TGCCGTGTCTTGCTAACGCCGATT ++ +#'#$$#$###%%##$$$$###### +@1378_25_2035/1 +CTGCGTGTTGGTGTCTACTGGGGT ++ +#%#'##$#$##&%#%$$$%#%#'# +@1378_28_770/1 +CTGCTATGCCGGCCGCCTGCCCCT ++ +%##(#$$#%$#$%###$%%$##$# +@1378_33_1945/1 +GGGGGGGGGGGGGGGGGGGGGGGG ++ ++9;64287788752796/576352 +@1378_34_789/1 +ATGGTGGCTGACGCGTTTGACTGT ++ +#$##%#$##$&$#%##$##$###$ +@1378_35_263/1 +GGGCGGGGGGCGGCGGGGGGGGGG ++ +),,#%'$)'-(%&%'%',%%&&(& +@1378_43_186/1 +ATACTAGTTGGGACGCGTTGTGCT ++ +#$(4%$########$#$###$$$# +@1378_51_1671/1 +TGGGTAGGTGCGGCTCTTGCGGTC ++ +##$#$%###%%#%#$##$$##### +@1378_56_324/1 +AGGCTTGGATTGTTGCGCTGACCT ++ +/+#**###%%##%#####$$$#$# +@1378_56_773/1 +TGTCGTGAGGTCACTTATCCCCAT ++ +&%#%##%%#####&#$%##$%##$ +@1378_62_2027/1 +CTTCCACGATCTGCTCGCTGTGGT ++ +(#&&$##$$#$%#%$$$#$###'# +@1378_62_2029/1 +TCTGGGCTGTCTTCGGGTCGGTGT ++ +$%$$####$##$$#)##%%#$### +@1378_67_1795/1 +TAGTGGGCTAGTCCGGGATCTACC ++ +B@C2@@?;-@@1>'A@@91@.@1' +@1378_68_466/1 +GTGATCGTCGGTGCCAGTCCCTGT ++ +#(%)+##$#$#%#+$%##$##### +@1378_68_1692/1 +GGTCTTCTGGGCACGGGCCAAGTT ++ +###)%'&%##$###%#######$# +@1378_80_664/1 +CTGCTTTGATCCCCGGTGGAGCAC ++ +7#%###$$6#######$##$$$## +@1378_85_1786/1 +ATACTATGTCGATCTGTAAAAAAA ++ +)&.)#3%@$&%-,2#&+.-%0&./ +@1378_86_1011/1 +CTACGTTATTGCTCTGTTTGTCCT ++ +######$%##$$$%###%#$#### +@1378_86_1789/1 +CGAATATCCCCTGGTTTAGAAGTA ++ +61?1;@/>@<=/(.7'(<5@=%$= +@1378_91_1596/1 +TTAGCGGTTGACTATCTGCTGACA ++ +*&+'#9'(%*'#//,&<),/)'*# +@1378_94_1595/1 +CGTGCGACAGCCCATGTTTTCAGA ++ +-=..5,3826&*+.+#+#%%6;%# +@1378_95_1039/1 +CGGCGTCCATCTTCGCCTTGAGAT ++ +$##.#$##$$#%$#$%%$###$)$ +@1378_95_1767/1 +ACACGCACCATCTGGCGGCTAACC ++ +86<65<4<C27/$5+#;-$+#2%$ +@1378_96_1037/1 +ATCCCCCAAGATGCCTGTTGATTG ++ +$#$'##$$$#%$$#%###+##$#$ +@1378_96_1764/1 +TACACCGGAACCTTGCACTAAAGC ++ +6-9'<#+81?+*<8-=69&6;*3) +@1378_98_1574/1 +GTTCTGCCGGTGTCTGTGGCGGGC ++ +$$#+&$$####%$$$###$%#%%# +@1378_107_1647/1 +AGGCCTACTACGCGTCATTGATAG ++ +&#$$#$(.#%#$$####&$%##($ +@1378_111_829/1 +TGCGGCACTTGCTTCTTCGTATTT ++ +%#%##%#$%#$#%###$$##&#$$ +@1378_111_1900/1 +TCCCCTCGCTCGGCTCTGTGCTGT ++ +$&%*$#(#)##$#'##%(##$#$% +@1378_112_1483/1 +TGTCCAGCTATGCGGCTTCCTCCT ++ +%#$+#%#&#$#####%####%$## +@1378_125_1287/1 +TGTCTCTGGGGGGCCTGGTTAGGT ++ +$##13$'%#$###$$###$$$#&# +@1378_126_468/1 +TCTCCTGATTAGACATATCTCCGT ++ +7>@318?=,=8@49579?..7,7) +@1378_127_664/1 +AGAGGTTGGTGTCTTGTCGCAGCT ++ +##'#$######$$%######$$$# +@1378_129_463/1 +CCTGTGGGCCAAGCCCAATGAAAG ++ +8),,<6(--)<()5&.&/8+;.2+ +@1378_129_875/1 +TTTCTATGGCTTACGCTGTCTGCC ++ +#$($##%####%$#$#####$### +@1378_140_1251/1 +ATCCTAGCGCGGTGTCTTGGGGAC ++ +#$%1#$$$##$##$#$#$##$%$$ +@1378_141_809/1 +TGTCCTCCAGTGTCTGTTGGGTGT ++ +%&,-##$$#(%###$#$$'###'# +@1378_144_983/1 +AGCGCCCGGTTGGTGCGGCTCGTC ++ +-$(&%*$#*#))#$$$#%%$#$## +@1378_153_270/1 +AGTCCTTGTCCCCTGGGTTTTCCC ++ ++''$#&%$%#$##&$$($#&#$$# +@1378_155_1689/1 +TTGGGAGGGAGAGAGACTAGACCG ++ +?4@@4<<??<?-@4@=4<5<?:/> +@1378_157_1580/1 +TGGGCCTCGGTGCCCTTGGTCTGT ++ +#%)$##'#$$$&#####%#$#$## +@1378_161_317/1 +TTGGCCGGCAACCCCGGTACCTAA ++ +7<,<'@)@>.)2@/')'&(?/-<( +@1378_177_735/1 +AGTACGCCATGTATTTGCGACCAG ++ +=?@?C<88@=>:7>@55/.,416, +@1378_181_1684/1 +CGACTCCCGCATTCACGGTCAAGT ++ +&*#,##$#&$*$$#$#$$$#%$## +@1378_187_1407/1 +TGGCGTCCACTCGTGGGTCTATCG ++ +$#$'%#$%$%&$%#####$#$#%# +@1378_203_721/1 +CCTAAATAACCCAGGGTAAAAGAT ++ +9<1+2+1@=259@+;2.71&@+2& +@1378_206_2039/1 +CCCCCCCCCCCCCCCCCCCCCCCC ++ +9@?8>;>>><<994;=895895-8 diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_paired_F3.csfasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_paired_F3.csfasta Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,99 @@ +# Fri Jan 23 21:37:16 2009 /share/apps/corona/bin/filter_fasta.pl --output=/data/results/0176/0176_20090114_1/skmusc_cov_25/results.01/primary.20090124033450140 --name=0176_20090114_1_skmusc_cov_25_ --tag=F3 --minlength=25 --mask=1111111111111111111111111 --prefix=T /data/results/0176/0176_20090114_1/skmusc_cov_25/jobs/postPrimerSetPrimary.1708/rawseq +# Cwd: /home/pipeline +# Title: 0176_20090114_1_skmusc_cov_25_ +>1378_11_329_F3 +T0020112221222232212331223 +>1378_17_1788_F3 +T0321123231332130012112033 +>1378_25_2035_F3 +T0132123233223231301322223 +>1378_28_770_F3 +T0132130321122112113211113 +>1378_33_1945_F3 +T2222222222222222222222222 +>1378_34_789_F3 +T0032232213201212333201323 +>1378_35_263_F3 +T0222122222212212222222222 +>1378_43_186_F3 +T0030130233222012123323213 +>1378_51_1671_F3 +T0322230223212213133212231 +>1378_56_324_F3 +T0022133220332332121320113 +>1378_56_773_F3 +T2323123202231013303111103 +>1378_62_2027_F3 +T0133110120313213121323223 +>1378_62_2029_F3 +T0313222132313312223122323 +>1378_67_1795_F3 +T0302322213023112220313011 +>1378_68_466_F3 +T0232031231223211023111323 +>1378_68_1692_F3 +T0223133132221012221100233 +>1378_80_664_F3 +T2132133320311112232202101 +>1378_85_1786_F3 +T0030130323120313230000000 +>1378_86_1011_F3 +T0130123303321313233323113 +>1378_86_1789_F3 +T0120030311113223330200230 +>1378_91_1596_F3 +T3330212233201303132132010 +>1378_94_1595_F3 +T2123212010211103233331020 +>1378_95_1039_F3 +T0122123110313312113320203 +>1378_95_1767_F3 +T2010121011031322122130011 +>1378_96_1037_F3 +T0031111100203211323320332 +>1378_96_1764_F3 +T0301011220011332101300021 +>1378_98_1574_F3 +T0233132112232313232212221 +>1378_107_1647_F3 +T0022113013012123103320302 +>1378_111_829_F3 +T0321221013321331331230333 +>1378_111_1900_F3 +T0311113121312213132321323 +>1378_112_1483_F3 +T0323110213032122133113113 +>1378_125_1287_F3 +T0323131322222211322330223 +>1378_126_468_F3 +T2313113203302010303131123 +>1378_127_664_F3 +T0020223322323133231210213 +>1378_129_463_F3 +T3113232221100211100320002 +>1378_129_875_F3 +T0333130322133012132313211 +>1378_140_1251_F3 +T0031130212122323133222201 +>1378_141_809_F3 +T0323113110232313233222323 +>1378_144_983_F3 +T1021211122332232122131231 +>1378_153_270_F3 +T0023113323111132223333111 +>1378_155_1689_F3 +T0332220222020202013020112 +>1378_157_1580_F3 +T3322211312232111332231323 +>1378_161_317_F3 +T1332211221001111223011300 +>1378_177_735_F3 +T1023012110323033321201102 +>1378_181_1684_F3 +T0120131112103310122310023 +>1378_187_1407_F3 +T0322123110131232223130312 +>1378_203_721_F3 +T0113000300111022230000203 +>1378_206_2039_F3 +T1111111111111111111111111 diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_paired_F3_QV.qualsolid --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_paired_F3_QV.qualsolid Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,99 @@ +# Fri Jan 23 21:37:16 2009 /share/apps/corona/bin/filter_fasta.pl --output=/data/results/0176/0176_20090114_1/skmusc_cov_25/results.01/primary.20090124033450140 --name=0176_20090114_1_skmusc_cov_25_ --tag=F3 --minlength=25 --mask=1111111111111111111111111 --prefix=T /data/results/0176/0176_20090114_1/skmusc_cov_25/jobs/postPrimerSetPrimary.1708/rawseq +# Cwd: /home/pipeline +# Title: 0176_20090114_1_skmusc_cov_25_ +>1378_11_329_F3 +7 4 2 2 10 6 2 2 2 2 2 2 2 4 2 2 2 3 2 3 2 2 3 7 2 +>1378_17_1788_F3 +6 2 6 2 3 3 2 3 2 2 2 4 4 2 2 3 3 3 3 2 2 2 2 2 2 +>1378_25_2035_F3 +9 2 4 2 6 2 2 3 2 3 2 2 5 4 2 4 3 3 3 4 2 4 2 6 2 +>1378_28_770_F3 +2 4 2 2 7 2 3 3 2 4 3 2 3 4 2 2 2 3 4 4 3 2 2 3 2 +>1378_33_1945_F3 +18 10 24 26 21 19 17 23 22 22 23 23 22 20 17 22 24 21 14 20 22 21 18 20 17 +>1378_34_789_F3 +2 2 3 2 2 4 2 3 2 2 3 5 3 2 4 2 2 3 2 2 3 2 2 2 3 +>1378_35_263_F3 +3 8 11 11 2 4 6 3 8 6 12 7 4 5 4 6 4 6 11 4 4 5 5 7 5 +>1378_43_186_F3 +2 2 3 7 19 4 3 2 2 2 2 2 2 2 2 3 2 3 2 2 2 3 3 3 2 +>1378_51_1671_F3 +2 2 2 3 2 3 4 2 2 2 4 4 2 4 2 3 2 2 3 3 2 2 2 2 2 +>1378_56_324_F3 +3 14 10 2 9 9 2 2 2 4 4 2 2 4 2 2 2 2 2 3 3 3 2 3 2 +>1378_56_773_F3 +9 5 4 2 4 2 2 4 4 2 2 2 2 2 5 2 3 4 2 2 3 4 2 2 3 +>1378_62_2027_F3 +9 7 2 5 5 3 2 2 3 3 2 3 4 2 4 3 3 3 2 3 2 2 2 6 2 +>1378_62_2029_F3 +4 3 4 3 3 2 2 2 2 3 2 2 3 3 2 8 2 2 4 4 2 3 2 2 2 +>1378_67_1795_F3 +33 33 31 34 17 31 31 30 26 12 31 31 16 29 6 32 31 31 24 16 31 13 31 16 6 +>1378_68_466_F3 +4 2 7 4 8 10 2 2 3 2 3 2 4 2 10 3 4 2 2 3 2 2 2 2 2 +>1378_68_1692_F3 +3 2 2 2 8 4 6 5 4 2 2 3 2 2 2 4 2 2 2 2 2 2 2 3 2 +>1378_80_664_F3 +8 22 2 4 2 2 2 3 3 21 2 2 2 2 2 2 2 3 2 2 3 3 3 2 2 +>1378_85_1786_F3 +6 8 5 13 8 2 18 4 31 3 5 4 12 11 17 2 5 10 13 12 4 15 5 13 14 +>1378_86_1011_F3 +2 2 2 2 2 2 2 3 4 2 2 3 3 3 4 2 2 2 4 2 3 2 2 2 2 +>1378_86_1789_F3 +31 21 16 30 16 26 31 14 29 31 27 28 14 7 13 22 6 7 27 20 31 28 4 3 28 +>1378_91_1596_F3 +4 9 5 10 6 2 24 6 7 4 9 6 2 14 14 11 5 27 8 11 14 8 6 9 2 +>1378_94_1595_F3 +8 12 28 13 13 20 11 18 23 17 21 5 9 10 13 10 2 10 2 4 4 21 26 4 2 +>1378_95_1039_F3 +2 3 2 2 13 2 3 2 2 3 3 2 4 3 2 3 4 4 3 2 2 2 3 8 3 +>1378_95_1767_F3 +12 23 21 27 21 20 27 19 27 34 17 22 14 3 20 10 2 26 12 3 10 2 17 4 3 +>1378_96_1037_F3 +2 3 2 3 6 2 2 3 3 3 2 4 3 3 2 4 2 2 2 10 2 2 3 2 3 +>1378_96_1764_F3 +29 21 12 24 6 27 2 10 23 16 30 10 9 27 23 12 28 21 24 5 21 26 9 18 8 +>1378_98_1574_F3 +2 3 3 2 10 5 3 3 2 2 2 2 4 3 3 3 2 2 2 3 4 2 4 4 2 +>1378_107_1647_F3 +2 5 2 3 3 2 3 7 13 2 4 2 3 3 2 2 2 2 5 3 4 2 2 7 3 +>1378_111_829_F3 +2 4 2 4 2 2 4 2 3 4 2 3 2 4 2 2 2 3 3 2 2 5 2 3 3 +>1378_111_1900_F3 +10 3 5 4 9 3 2 7 2 8 2 2 3 2 6 2 2 4 7 2 2 3 2 3 4 +>1378_112_1483_F3 +9 4 2 3 10 2 4 2 5 2 3 2 2 2 2 2 4 2 2 2 2 4 3 2 2 +>1378_125_1287_F3 +4 3 2 2 16 18 3 6 4 2 3 2 2 2 3 3 2 2 2 3 3 3 2 5 2 +>1378_126_468_F3 +23 22 29 31 18 16 23 30 28 11 28 23 31 19 24 20 22 24 30 13 13 22 11 22 8 +>1378_127_664_F3 +9 2 2 6 2 3 2 2 2 2 2 2 3 3 4 2 2 2 2 2 2 3 3 3 2 +>1378_129_463_F3 +4 23 8 11 11 27 21 7 12 12 8 27 7 8 20 5 13 5 14 23 10 26 13 17 10 +>1378_129_875_F3 +2 2 3 7 3 2 2 4 2 2 2 2 4 3 2 3 2 2 2 2 2 3 2 2 2 +>1378_140_1251_F3 +12 2 3 4 16 2 3 3 3 2 2 3 2 2 3 2 3 2 3 2 2 3 4 3 3 +>1378_141_809_F3 +7 4 5 11 12 2 2 3 3 2 7 4 2 2 2 3 2 3 3 6 2 2 2 6 2 +>1378_144_983_F3 +11 12 3 7 5 4 9 3 2 9 2 8 8 2 3 3 3 2 4 4 3 2 3 2 2 +>1378_153_270_F3 +8 10 6 6 3 2 5 4 3 4 2 3 2 2 5 3 3 7 3 2 5 2 3 3 2 +>1378_155_1689_F3 +31 30 19 31 31 19 27 27 30 30 27 30 12 31 19 31 28 19 27 20 27 30 25 14 29 +>1378_157_1580_F3 +3 2 4 8 3 2 2 6 2 3 3 3 5 2 2 2 2 2 4 2 3 2 3 2 2 +>1378_161_317_F3 +8 22 27 11 27 6 31 8 31 29 13 8 17 31 14 6 8 6 5 7 30 14 12 27 7 +>1378_177_735_F3 +31 28 30 31 30 34 27 23 23 31 28 29 25 22 29 31 20 20 14 13 11 19 16 21 11 +>1378_181_1684_F3 +13 5 9 2 11 2 2 3 2 5 3 9 3 3 2 3 2 3 3 3 2 4 3 2 2 +>1378_187_1407_F3 +2 3 2 3 6 4 2 3 4 3 4 5 3 4 2 2 2 2 2 3 2 3 2 4 2 +>1378_203_721_F3 +33 24 27 16 10 17 10 16 31 28 17 20 24 31 10 26 17 13 22 16 5 31 10 17 5 +>1378_206_2039_F3 +26 24 31 30 23 29 26 29 29 29 27 27 24 24 19 26 28 23 24 20 23 24 20 12 23 diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_paired_R3.csfasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_paired_R3.csfasta Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,99 @@ +# Mon Jan 19 06:35:55 2009 /share/apps/corona/bin/filter_fasta.pl --output=/data/results/0176/0176_20090114_1/skmusc_cov_25/results.01/primary.20090119120505312 --name=0176_20090114_1_skmusc_cov_25_ --tag=R3 --minlength=25 --mask=1111111111111111111111111 --prefix=G /data/results/0176/0176_20090114_1/skmusc_cov_25/jobs/postPrimerSetPrimary.1512/rawseq +# Cwd: /home/pipeline +# Title: 0176_20090114_1_skmusc_cov_25_ +>1378_11_329_R3 +G2233123221122322232333222 +>1378_17_1788_R3 +G3322232203232332312331032 +>1378_25_2035_R3 +G2232123122220222321323122 +>1378_28_770_R3 +G2223322202332212121232233 +>1378_33_1945_R3 +G2222222222222222222222222 +>1378_34_789_R3 +G3222133212330232020223323 +>1378_35_263_R3 +G2221222222222222222222222 +>1378_43_186_R3 +G3213022233322233321223222 +>1378_51_1671_R3 +G2223233233122032302123222 +>1378_56_324_R3 +G3213222320313002200323003 +>1378_56_773_R3 +G1313223122333122220232200 +>1378_62_2027_R3 +G2233221132211321123213212 +>1378_62_2029_R3 +G2221223232322321221323212 +>1378_67_1795_R3 +G1123211001201123113012202 +>1378_68_466_R3 +G1232310313202230002103323 +>1378_68_1692_R3 +G3323312033302231332113121 +>1378_80_664_R3 +G2323132123323031313223230 +>1378_85_1786_R3 +G3111302202123030112201202 +>1378_86_1011_R3 +G1022120322203033033330133 +>1378_86_1789_R3 +G1221102230130030003310010 +>1378_91_1596_R3 +G2213333310331223211333220 +>1378_94_1595_R3 +G3212223300111023011313221 +>1378_95_1039_R3 +G3233132321102232022301220 +>1378_95_1767_R3 +G2130033201122210021303300 +>1378_96_1037_R3 +G0132132221103332013301310 +>1378_96_1764_R3 +G3313332300111013302303331 +>1378_98_1574_R3 +G2022120232322222332333202 +>1378_107_1647_R3 +G3223132233130323322312013 +>1378_111_829_R3 +G2223133130322321300033023 +>1378_111_1900_R3 +G2210121133322213002112300 +>1378_112_1483_R3 +G3322023223232333213202110 +>1378_125_1287_R3 +G3320123222332311123202033 +>1378_126_468_R3 +G1000000122211222122013022 +>1378_127_664_R3 +G3312133321130323332331220 +>1378_129_463_R3 +G0000000000000000000000000 +>1378_129_875_R3 +G3201133301230332222233221 +>1378_140_1251_R3 +G3333113312323212321220232 +>1378_141_809_R3 +G3313123223331333333032323 +>1378_144_983_R3 +G2233123312322323012022232 +>1378_153_270_R3 +G2221123232122232302033220 +>1378_155_1689_R3 +G1121233122010002130210113 +>1378_157_1580_R3 +G3222033200222032303213022 +>1378_161_317_R3 +G2003110301110100002102211 +>1378_177_735_R3 +G2120211130001103202031220 +>1378_181_1684_R3 +G3333132332322333323322223 +>1378_187_1407_R3 +G3332223200031332312023220 +>1378_203_721_R3 +G1113330120310300011032011 +>1378_206_2039_R3 +G1111111111111111111111111 diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_paired_R3_QV.qualsolid --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_paired_R3_QV.qualsolid Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,99 @@ +# Mon Jan 19 06:35:55 2009 /share/apps/corona/bin/filter_fasta.pl --output=/data/results/0176/0176_20090114_1/skmusc_cov_25/results.01/primary.20090119120505312 --name=0176_20090114_1_skmusc_cov_25_ --tag=R3 --minlength=25 --mask=1111111111111111111111111 --prefix=G /data/results/0176/0176_20090114_1/skmusc_cov_25/jobs/postPrimerSetPrimary.1512/rawseq +# Cwd: /home/pipeline +# Title: 0176_20090114_1_skmusc_cov_25_ +>1378_11_329_R3 +2 2 2 2 3 3 2 3 2 3 5 2 2 2 2 2 3 6 3 2 3 2 2 2 3 +>1378_17_1788_R3 +2 2 3 2 3 2 2 2 3 2 3 2 2 2 2 2 2 2 3 2 3 2 2 2 2 +>1378_25_2035_R3 +2 2 2 2 2 2 2 4 2 3 4 2 3 3 2 2 2 7 3 2 2 2 5 5 4 +>1378_28_770_R3 +2 2 2 2 3 2 2 4 4 3 2 2 2 2 4 2 4 3 2 4 4 2 7 3 3 +>1378_33_1945_R3 +26 13 21 26 30 26 20 20 21 24 22 29 23 22 22 24 19 20 22 24 23 21 17 21 19 +>1378_34_789_R3 +3 2 2 2 4 3 4 3 4 4 2 2 2 3 2 2 2 2 3 2 2 2 3 2 5 +>1378_35_263_R3 +7 3 4 4 5 11 8 18 14 8 4 3 12 21 6 3 5 20 5 2 3 6 6 20 13 +>1378_43_186_R3 +2 3 4 2 3 2 2 2 2 2 2 2 2 4 2 2 4 2 3 2 2 2 6 2 6 +>1378_51_1671_R3 +2 2 2 4 3 3 2 2 2 2 3 2 2 2 2 2 2 2 2 3 2 2 15 2 2 +>1378_56_324_R3 +2 2 3 2 2 2 2 2 3 3 3 2 3 3 3 3 2 2 4 2 2 2 9 7 2 +>1378_56_773_R3 +4 2 2 4 4 2 5 3 2 2 2 3 2 3 2 2 4 3 2 2 2 2 4 4 3 +>1378_62_2027_R3 +10 9 2 2 8 11 14 4 2 2 3 8 2 4 2 2 16 3 2 6 4 13 2 5 2 +>1378_62_2029_R3 +8 14 3 3 3 28 7 2 2 2 2 4 2 2 2 2 8 3 3 4 3 12 5 4 2 +>1378_67_1795_R3 +33 31 31 13 30 22 31 31 31 13 31 25 31 32 27 27 29 29 28 29 22 15 27 31 22 +>1378_68_466_R3 +12 14 2 2 3 15 24 2 3 2 13 28 3 2 3 22 21 10 3 4 16 6 2 2 2 +>1378_68_1692_R3 +4 2 2 2 4 4 3 4 4 2 2 4 3 2 3 2 2 3 2 2 2 3 3 2 3 +>1378_80_664_R3 +4 4 2 2 4 11 4 3 3 2 5 3 3 2 2 2 3 2 3 4 2 2 6 4 2 +>1378_85_1786_R3 +5 11 6 5 14 4 14 31 11 5 16 11 5 6 14 8 5 11 21 5 5 16 8 7 7 +>1378_86_1011_R3 +11 25 3 2 2 2 8 4 2 2 3 24 3 2 2 2 16 3 3 2 3 17 2 2 2 +>1378_86_1789_R3 +19 16 13 20 16 14 5 4 2 6 3 11 9 30 4 2 21 5 15 3 3 20 11 17 15 +>1378_91_1596_R3 +11 6 29 4 14 18 4 28 7 8 23 6 2 13 4 30 20 15 3 5 20 29 4 8 4 +>1378_94_1595_R3 +18 11 23 31 21 6 29 23 27 22 21 29 31 31 7 12 29 24 8 22 6 9 24 19 16 +>1378_95_1039_R3 +6 5 2 2 11 13 14 2 3 5 8 21 2 2 10 11 6 2 3 3 15 7 2 2 3 +>1378_95_1767_R3 +8 6 11 11 17 6 7 16 15 14 29 25 28 14 22 8 13 18 5 4 21 5 8 5 21 +>1378_96_1037_R3 +13 6 3 2 10 2 7 2 2 12 4 20 2 2 10 9 5 2 2 2 12 13 3 3 3 +>1378_96_1764_R3 +13 19 28 6 17 23 29 13 31 19 23 23 16 33 23 15 22 25 23 17 17 29 4 8 16 +>1378_98_1574_R3 +3 10 4 4 3 2 8 2 2 4 2 2 3 2 2 2 2 4 2 2 2 3 4 3 2 +>1378_107_1647_R3 +3 2 2 2 6 3 3 2 3 3 3 7 2 4 2 2 2 7 2 3 2 2 3 4 2 +>1378_111_829_R3 +3 3 2 2 6 5 2 2 2 3 2 2 8 2 4 7 3 2 3 4 2 3 6 4 2 +>1378_111_1900_R3 +6 8 3 8 6 2 4 3 2 2 2 2 2 2 2 2 3 6 2 5 4 3 2 7 2 +>1378_112_1483_R3 +2 2 3 2 8 2 2 2 2 2 2 2 2 2 2 2 2 2 3 2 4 2 4 6 4 +>1378_125_1287_R3 +2 2 2 3 4 4 2 3 2 2 2 3 2 2 3 3 2 5 4 2 2 3 7 4 4 +>1378_126_468_R3 +30 31 31 33 31 31 31 29 31 17 29 16 10 30 25 24 17 8 29 31 23 5 5 27 10 +>1378_127_664_R3 +2 2 4 3 4 2 5 2 2 3 4 2 4 4 2 2 2 3 3 2 2 2 8 12 6 +>1378_129_463_R3 +13 11 8 5 17 15 4 23 6 7 5 18 20 12 9 9 9 16 12 17 5 10 20 16 15 +>1378_129_875_R3 +4 2 2 2 8 2 2 2 10 2 2 2 3 2 2 3 2 5 4 2 2 3 11 2 3 +>1378_140_1251_R3 +2 2 4 2 4 3 2 2 3 3 3 2 2 2 2 2 2 13 3 3 3 4 2 4 7 +>1378_141_809_R3 +2 2 2 4 8 2 2 3 3 2 2 2 2 2 4 3 2 3 2 4 4 2 6 2 2 +>1378_144_983_R3 +4 2 7 2 4 2 2 2 2 2 7 3 2 4 2 2 3 3 2 4 2 2 4 2 8 +>1378_153_270_R3 +4 4 3 2 2 7 3 2 2 2 2 2 2 5 2 2 3 5 3 3 3 3 4 2 2 +>1378_155_1689_R3 +31 31 19 31 31 28 21 28 31 31 27 19 25 31 31 21 23 16 31 31 31 13 17 19 13 +>1378_157_1580_R3 +2 2 4 3 5 4 2 3 3 6 4 3 4 2 3 2 2 9 2 4 3 3 3 3 2 +>1378_161_317_R3 +8 13 5 4 6 11 6 7 31 6 6 30 22 14 14 10 5 8 10 17 13 10 8 15 8 +>1378_177_735_R3 +31 31 31 33 29 31 32 34 24 31 32 27 33 23 31 28 24 29 22 31 20 8 29 11 15 +>1378_181_1684_R3 +2 3 2 2 6 3 4 6 2 2 4 2 2 3 4 3 2 3 3 2 2 2 2 3 9 +>1378_187_1407_R3 +2 2 2 2 2 5 2 2 3 3 2 2 2 3 2 2 2 2 2 4 2 2 4 4 8 +>1378_203_721_R3 +24 24 27 5 13 14 16 5 31 26 16 17 6 8 30 27 16 6 11 30 14 8 5 14 13 +>1378_206_2039_R3 +31 30 28 29 29 30 28 25 26 27 29 29 19 25 25 27 28 20 21 16 24 24 7 15 20 diff -r 54aa0543925b -r 61dd78cafa09 test-data/s2fq_phiX.csfasta --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_phiX.csfasta Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,288 @@ +>1831_573_1004_F3 +T00030133312212111300011021310132222 +>1831_573_1050_F3 +T03330322230322112131010221102122113 +>1831_573_1067_F3 +T00023032023103330112220321200200002 +>1831_573_1219_F3 +T11211130300300301021212330201121310 +>1831_573_1242_F3 +T02132003121011302100130302112221121 +>1831_573_1333_F3 +T00200312330110101013212313222303112 +>1831_573_1362_F3 +T21203131001102231121211101111321131 +>1831_573_1448_F3 +T23101211223113320132212331313312022 +>1831_573_1490_F3 +T31312310323301210002210123101021011 +>1831_573_1523_F3 +T10322001220012223202202222001230222 +>1831_573_1578_F3 +T21202302100010020121100311022120111 +>1831_573_1647_F3 +T10222233301013033120132223202022123 +>1831_573_1684_F3 +T13310013212312012302121010221231123 +>1831_573_1769_F3 +T33220123030232212032021032302233131 +>1831_573_1853_F3 +T11000012111222211310103212122102331 +>1831_573_1943_F3 +T20300123032210232001222122001132111 +>1831_573_1977_F3 +T22212302221310332321002303112011311 +>1831_574_109_F3 +T13122332123301331032220222133301033 +>1831_574_148_F3 +T01200113123030012202302312200010231 +>1831_574_185_F3 +T21123333211302300321312212102123121 +>1831_574_243_F3 +T30221011230013102201033131203302330 +>1831_574_257_F3 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diff -r 54aa0543925b -r 61dd78cafa09 tool_conf.xml.sample --- a/tool_conf.xml.sample Fri Jul 31 11:38:03 2009 -0400 +++ b/tool_conf.xml.sample Fri Jul 31 11:48:37 2009 -0400 @@ -77,6 +77,8 @@ <tool file="maf/maf_to_bed.xml" /> <tool file="maf/maf_to_fasta.xml" /> <tool file="fasta_tools/tabular_to_fasta.xml" /> + <tool file="next_gen_conversion/solid_to_fastq.xml" /> + <tool file="next_gen_conversion/fastq_conversions.xml" /> </section> <section name="Extract Features" id="features"> <tool file="filters/ucsc_gene_bed_to_exon_bed.xml" /> diff -r 54aa0543925b -r 61dd78cafa09 tools/next_gen_conversion/fastq_conversions.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/next_gen_conversion/fastq_conversions.py Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,41 @@ +#! /usr/bin/python + +""" +Performs various conversions around Sanger FASTQ data + +usage: %prog [options] + -c, --command=c: Command to run + -i, --input=i: Input file to be converted + -o, --outputFastqsanger=o: FASTQ Sanger converted output file for sol2std + -s, --outputFastqsolexa=s: FASTQ Solexa converted output file + -f, --outputFasta=f: FASTA converted output file + +usage: %prog command input_file output_file +""" + +import os, sys, tempfile +from galaxy import eggs +import pkg_resources; pkg_resources.require( "bx-python" ) +from bx.cookbook import doc_optparse + +def stop_err( msg ): + sys.stderr.write( "%s\n" % msg ) + sys.exit() + +def __main__(): + #Parse Command Line + options, args = doc_optparse.parse( __doc__ ) + + cmd = "fq_all2std.pl %s %s > %s" + if options.command == 'sol2std': + cmd = cmd % (options.command, options.input, options.outputFastqsanger) + elif options.command == 'std2sol': + cmd = cmd % (options.command, options.input, options.outputFastqsolexa) + elif options.command == 'fq2fa': + cmd = cmd % (options.command, options.input, options.outputFasta) + try: + os.system(cmd) + except Exception, eq: + stop_err("Error converting data format.\n" + str(eq)) + +if __name__=="__main__": __main__() diff -r 54aa0543925b -r 61dd78cafa09 tools/next_gen_conversion/fastq_conversions.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/next_gen_conversion/fastq_conversions.xml Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,133 @@ +<tool id="fastq_conversions" name="FASTQ Conversions" version="1.0.0"> + <description>converts between FASTQ data and other data formats</description> + <command interpreter="python"> + fastq_conversions.py + --command=$conversionType.type + --input=$input + #if $conversionType.type == "sol2std": + --outputFastqsanger=$outputFastqsanger + #else: + --outputFastqsanger="None" + #end if + #if $conversionType.type == "std2sol": + --outputFastqsolexa=$outputFastqsolexa + #else: + --outputFastqsolexa="None" + #end if + #if $conversionType.type == "fq2fa": + --outputFasta=$outputFasta + #else: + --outputFasta="None" + #end if + </command> + <inputs> + <conditional name="conversionType"> + <param name="type" type="select" label="What type of conversion do you want to do?"> + <option value="sol2std">Solexa/Illumina FASTQ to standard Sanger FASTQ</option> + <option value="std2sol">Standard Sanger FASTQ to Solexa/Illumina FASTQ</option> + <option value="fq2fa">Various FASTQ to FASTA</option> + </param> + <when value="sol2std"> + <param name="input" type="data" format="fastqsolexa" label="File to convert" /> + </when> + <when value="std2sol"> + <param name="input" type="data" format="fastqsanger" label="File to convert" /> + </when> + <when value="fq2fa"> + <param name="input" type="data" format="fastqsolexa, fastqsanger" label="File to convert" /> + </when> + </conditional> + </inputs> + <outputs> + <data name="outputFastqsanger" format="fastqsanger"> + <filter>conversionType['type'] == 'sol2std'</filter> + </data> + <data name="outputFastqsolexa" format="fastqsolexa"> + <filter>conversionType['type'] == 'std2sol'</filter> + </data> + <data name="outputFasta" format="fasta"> + <filter>conversionType['type'] == 'fq2fa'</filter> + </data> + </outputs> + <tests> + <test> + <param name="type" value="sol2std" /> + <param name="input" value="bwa_phiX_sanger.fastq" ftype="fastqsolexa" /> + <output name="outputFastqsanger" file="fastq_conv_out1.fastqsanger" /> + </test> + <test> + <param name="type" value="std2sol" /> + <param name="input" value="1.fastqsanger" ftype="fastqsanger" /> + <output name="outputFastqsolexa" file="fastq_conv_out2.fastqsolexa" /> + </test> + <test> + <param name="type" value="fq2fa" /> + <param name="input" value="1.fastqsanger" ftype="fastqsanger" /> + <output name="outputFasta" file="fastq_conv_out4.fasta" /> + </test> + </tests> + <help> +**What it does** + +This tool offers several conversions options relating to the FASTQ format. + +----- + +**Examples** + +- Converting the Solexa/Illumina FASTQ data:: + + @081017-and-081020:1:1:1715:1759 + GGACTCAGATAGTAATCCACGCTCCTTTAAAATATC + + + II#IIIIIII$5+.(9IIIIIII$%*$G$A31I&&B + +- will produce the following Sanger FASTQ data:: + + @081017-and-081020:1:1:1715:1759 + GGACTCAGATAGTAATCCACGCTCCTTTAAAATATC + + + ++!+++++++!!!!!"+++++++!!!!)!%!!+!!%! + +- Converting standard Sanger FASTQ:: + + @1831_573_1004/1 + AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG + + + ><C&&9952+C>5<.?<79,=42<292:<(9/-7 + @1831_573_1050/1 + TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT + + + ;@@17?@=>7??@A8?==@4A?A4)&+.'&+'1, + +- will produce the following Solexa/Illumina FASTQ data:: + + @1831_573_1004/1 + AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG + + + ][bEEXXTQJb]T[M^[VXK\SQ[QXQY[GXNLV + @1831_573_1050/1 + TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT + + + Z__PV^_\]V^^_`W^\\_S`^`SHEJMFEJFPK + +- Converting the Sanger FASTQ data:: + + @1831_573_1004/1 + AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG + + + ><C&&9952+C>5<.?<79,=42<292:<(9/-7 + @1831_573_1050/1 + TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT + + + ;@@17?@=>7??@A8?==@4A?A4)&+.'&+'1, + +- will produce the following FASTA data:: + + >1831_573_1004/1 + AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG + >1831_573_1050/1 + TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT + + </help> +</tool> diff -r 54aa0543925b -r 61dd78cafa09 tools/next_gen_conversion/solid_to_fastq.py --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/next_gen_conversion/solid_to_fastq.py Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,53 @@ +#! /usr/bin/python + +""" +Converts SOLiD data to Sanger FASTQ format. + +usage: %prog [options] + -i, --input1=i: Forward reads file + -q, --input2=q: Forward qual file + -I, --input3=I: Reverse reads file + -Q, --input4=Q: Reverse qual file + -o, --output1=o: Forward output + -r, --output2=r: Reverse output + +usage: %prog forward_reads_file forwards_qual_file reverse_reads_file(or_None) reverse_qual_file(or_None) output_file ouptut_id output_dir +""" + +import os, sys, tempfile +from galaxy import eggs +import pkg_resources; pkg_resources.require( "bx-python" ) +from bx.cookbook import doc_optparse + +def stop_err( msg ): + sys.stderr.write( "%s\n" % msg ) + sys.exit() + +def __main__(): + #Parse Command Line + options, args = doc_optparse.parse( __doc__ ) + # if paired-end data (have reverse input files) + if options.input3 != "None" and options.input4 != "None": + tmpf = tempfile.NamedTemporaryFile() #forward reads + tmpr = tempfile.NamedTemporaryFile() #reverse reads + + cmd1 = "bwa_solid2fastq_modified.pl 'yes' %s %s %s %s %s %s %s 2>&1" %(tmpf.name,tmpr.name,None,options.input1,options.input2,options.input3,options.input4) + try: + os.system(cmd1) + os.system('gunzip -c %s >> %s' %(tmpf.name,options.output1)) + os.system('gunzip -c %s >> %s' %(tmpr.name,options.output2)) + + except Exception, eq: + stop_err("Error converting data to fastq format.\n" + str(eq)) + # if single-end data + else: + tmpf = tempfile.NamedTemporaryFile() + cmd1 = "bwa_solid2fastq_modified.pl 'no' %s %s %s %s %s %s %s 2>&1" % (tmpf.name, None, None, options.input1, options.input2, None, None) + try: + os.system(cmd1) + os.system('gunzip -c %s >> %s' % (tmpf.name, options.output1)) + tmpf.close() + except Exception, eq: + stop_err("Error converting data to fastq format.\n" + str(eq)) + +if __name__=="__main__": __main__() diff -r 54aa0543925b -r 61dd78cafa09 tools/next_gen_conversion/solid_to_fastq.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/next_gen_conversion/solid_to_fastq.xml Fri Jul 31 11:48:37 2009 -0400 @@ -0,0 +1,101 @@ +<tool id="solid_to_fastq" name="SOLiD-to-FASTQ" version="1.0.0"> + <description>converts SOLiD data to FASTQ data</description> + <command interpreter="python"> + solid_to_fastq.py + --input1=$input1 + --input2=$input2 + #if $paired.pairedSingle == "single": + --input3="None" + --input4="None" + #else: + --input3=$input3 + --input4=$input4 + #end if + --output1=$output1 + #if $paired.pairedSingle == "single": + --output2="None" + #else: + --output2=$output2 + #end if + </command> + <inputs> + <conditional name="paired"> + <param name="pairedSingle" type="select" label="Is this library mate-paired?"> + <option value="single">Single</option> + <option value="paired">Paired</option> + </param> + <when value="single"> + <param name="input1" type="data" format="csfasta" label="F3 read file" /> + <param name="input2" type="data" format="qualsolid" label="F3 qual file" /> + </when> + <when value="paired"> + <param name="input1" type="data" format="csfasta" label="F3 read file" /> + <param name="input2" type="data" format="qualsolid" label="F3 qual file" /> + <param name="input3" type="data" format="csfasta" label="R3 read file" /> + <param name="input4" type="data" format="qualsolid" label="R3 qual file" /> + </when> + </conditional> + </inputs> + <outputs> + <!-- Variable number of outputs. Either one (for single-end) or two (for paired-end) --> + <data name="output1" format="tabular"/> + <data name="output2" format="tabular"> + <filter>paired['pairedSingle'] == 'paired'</filter> + </data> + </outputs> + <tests> + <test> + <param name="pairedSingle" value="single" /> + <param name="input1" value="s2fq_phiX.csfasta" ftype="csfasta" /> + <param name="input2" value="s2fq_phiX.qualsolid" ftype="qualsolid" /> + <output name="output1" file="s2fq_out1.tabular" /> + </test> + <!-- testing framework does not deal with multiple outputs yet + <test> + <param name="pairedSingle" value="paired" /> + <param name="input1" value="s2fq_paired_F3.csfasta" ftype="csfasta" /> + <param name="input2" value="s2fq_paired_F3_QV.qualsolid" ftype="qualsolid" /> + <param name="input3" value="s2fq_paired_R3.csfasta" ftype="csfasta" /> + <param name="input4" value="s2fq_paired_R3_QV.qualsolid" ftype="qualsolid" /> + <output name="output1" file="s2fq_out2.tabular" /> + <output name="output2" file="s2fq_out3.tabular" /> + </test> + --> + </tests> + <help> + +**What it does** + +This tool takes reads and quality files and converts them to FASTQ data ( Sanger variant ). Note that it also converts sequences to base pairs. + +----- + +**Example** + +- Converting the following sequences:: + + >seq1 + T00030133312212111300011021310132222 + >seq2 + T03330322230322112131010221102122113 + +- and quality scores:: + + >seq1 + 4 29 34 34 32 32 24 24 20 17 10 34 29 20 34 13 30 34 22 24 11 28 19 17 34 17 24 17 25 34 7 24 14 12 22 + >seq2 + 8 26 31 31 16 22 30 31 28 29 22 30 30 31 32 23 30 28 28 31 19 32 30 32 19 8 32 10 13 6 32 10 6 16 11 + +- will produce the following Sanger FASTQ data:: + + @seq1 + AATACTTTCGGCGCCCTAAACCAGCTCACTGGGG + + + >CCAA9952+C>5C.?C79,=42C292:C(9/-7 + @seq2 + TTTATGGGTATGGCCGCTCACAGGCCAGCGGCCT + + + ;@@17?@=>7??@A8?==@4A?A4)A+.'A+'1, + + </help> +</tool>