Velvetoptimizer won't read in my paired, illumina file
Hi. I have an illumina 1.9 file that I renamed, groomed, and interlaced to get to its current state (see first 2 sets of paired sequences below) @1 TTTTTTATCAAAAAAAATGCATATACGAAATTTTTATTTTTACTTTTAAAAATTGAAAGTTGAAAATTTTAAATAAATGAAAAATAAATTAAACCAATTA +1 @CCCDDC?DDDFHHHGIIGJJIIIHEIJHGECIDHHHGIEGIGDHDGIHGHF4IGIHEGIJJJJIGHCFIJIHDHF4IIJIHHIGIGFHGHHFFFDF@@B @1 TTTATCAAAATATTTTCAAGCTAATTACAAGACATACTTACTTAAGTAAATTAAGATATATGGTTAATATTGCATTAAATAAGCTTTAATAAAGAGATAA +1 @CCDFFDFHHHGHJJJIIJJJIIIJJIIJIHIGGIBHGIHIHHIGIIIGHEIIICHGGHDHGIHHDIGIIGGGGICHGGDGFEG>EADECC7?CCDFD@C @2 ATTTATACATCTGTTGAACAAGTTATTAATTCTGTTTTCGATCTTTTTAATGTTATCTTTTACTTGTCCTCCCACATAGATCTTCATTCCTAAATTTATA +2 CCCC;,(>5(5((>3;AC>@@;3;?:DEHA;=A@55)((8)(6HGB8F?9<B?99)CHGGFCC?1)8)A+EC:CAA:F@FECA+B9C:BD4DDB2+;=+B @2 TATCCATACAAACTTATCATTCACTTTCAGTAGTAACCTATTCTTATTTTGTTTAACTTCAAATACCTTAAAAGGCCCTTCCCAAACACTATCTGACTTC +2 =8?;A=B?CFBBHGG2ACAC@@9E?F@HIIGHGI<+1?E9EEDHEC<GHBGHIGBFHCHFG@BB8BFFHCG:=;(=@D=C3?.;=;?DEC>C3>C(>>C> Now I want to do an initial assembly with velvetoptimizer on my local instance of Galaxy. When I run velvetoptimizer, calling this file a fastq file of short paired reads, it fails and I get this error message: SyntaxError: unexpected character after line continuation character Any ideas on what I'm doing wrong? Thanks! -Lucinda
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Lucinda Lawson