I wanted to plug two tools that we have wrapped up for Galaxy: whole genome simulation of NGS reads, as well as the evaluation of the resulting mappings of those reads. This could be very useful for those that want to evaluate mapping algorithms, or experiment with simulated NGS data. Check out the "dwgsim" and "dwgsim_eval" tools at http://toolshed.g2.bx.psu.edu/ The tools and wrapper code can also be found at http://dnaa.sf.net, and described at https://sourceforge.net/apps/mediawiki/dnaa/index.php?title=Whole_Genome_Sim... Feedback and comments are very welcome, Nils Homer