Hello ... I've installed a Galaxy server on one of our local machines (X86_64 linux running CentOS 5). When I go to modules that have a "Select a reference genome:" option, the dropboxes are empty. I did find this thread: http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-August/003138.html But it's still not making sense to me. To test things out, I've downloaded the chr*.fa.gz files for hg18 from UCSC. Based on the advice in this thread, I've used faToTwoBit on each of the .fa files. These files are all sitting in /home/galaxy/local/galaxy/genomes/hg18/ This poster says to add the location to tool-data/alignseq.loc, but this file's comments says that it uses axt files for alignment data and nib files for seqs - these are clearly neither. I added the following entry to my faseq.loc file: hg18 /home/galaxy/local/genomes/hg18 and to my twobit.loc: hg18 /home/galaxy/local/galaxy/genomes/hg18 But even after restarting the server hg18 isn't showing up in the reference genome. Clearly I'm doing something very wrong here, can anyone help me out here? Thanks -J
Hi, What type of data are you trying to add? For NGS references, see https://bitbucket.org/galaxy/galaxy-central/wiki/NGSLocalSetup . Let us know if that isn't enough info. Regards, Kelly On Feb 2, 2011, at 3:58 PM, Geoff Jentry wrote:
Hello ...
I've installed a Galaxy server on one of our local machines (X86_64 linux running CentOS 5). When I go to modules that have a "Select a reference genome:" option, the dropboxes are empty.
I did find this thread: http://lists.bx.psu.edu/pipermail/galaxy-dev/2010-August/003138.html
But it's still not making sense to me. To test things out, I've downloaded the chr*.fa.gz files for hg18 from UCSC. Based on the advice in this thread, I've used faToTwoBit on each of the .fa files. These files are all sitting in /home/galaxy/local/galaxy/ genomes/hg18/
This poster says to add the location to tool-data/alignseq.loc, but this file's comments says that it uses axt files for alignment data and nib files for seqs - these are clearly neither.
I added the following entry to my faseq.loc file: hg18 /home/galaxy/local/genomes/hg18
and to my twobit.loc: hg18 /home/galaxy/local/galaxy/genomes/hg18
But even after restarting the server hg18 isn't showing up in the reference genome. Clearly I'm doing something very wrong here, can anyone help me out here?
Thanks -J _______________________________________________ galaxy-dev mailing list galaxy-dev@lists.bx.psu.edu http://lists.bx.psu.edu/listinfo/galaxy-dev
What type of data are you trying to add? For NGS references, see https://bitbucket.org/galaxy/galaxy-central/wiki/NGSLocalSetup. Let us know if that isn't enough info.
Ah, thanks Kelly. I had not come across this page. Following these instructions, I was able to get a genome to show up in the drop down for "Map with Bowtie for Illumina". However, the same drop down is not showing my genome in "Map with Bowtie for SOLiD". Any idea as to why these would be different? Thanks -J
Hi, There are two things: first, the Bowtie for Illumina and Bowtie for SOLiD wrappers use different data tables (bowtie_indexes as opposed to bowtie_indexes_color), which refer to different loc files (bowtie_indexes.loc and bowtie_indexes_color.loc, respectively). Secondly, although this won't effect display in the dropdown, the index files will also need to be different for use with SOLiD reads (the -C (upper case) needs to be used when creating indexes for use with color-space data). In our case, the directory structure and index naming are standardized so it's easy to construct bowtie_indexes_color.loc based on bowtie_indexes.loc--we just add the cs subdirectory after the bowtie index directory in the path (the index files are named the same as base-space ones, so only their location is different). Regards, Kelly On Feb 3, 2011, at 11:21 AM, Geoff Jentry wrote:
What type of data are you trying to add? For NGS references, see https://bitbucket.org/galaxy/galaxy-central/wiki/NGSLocalSetup . Let us know if that isn't enough info.
Ah, thanks Kelly. I had not come across this page. Following these instructions, I was able to get a genome to show up in the drop down for "Map with Bowtie for Illumina". However, the same drop down is not showing my genome in "Map with Bowtie for SOLiD". Any idea as to why these would be different?
Thanks -J
There are two things: first, the Bowtie for Illumina and Bowtie for SOLiD wrappers use different data tables (bowtie_indexes as opposed to bowtie_indexes_color), which refer to different loc files (bowtie_indexes.loc and bowtie_indexes_color.loc, respectively). Secondly, although this won't
Ahh right. I totally missed that. It's working now, thanks.
participants (2)
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Geoff Jentry
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Kelly Vincent