Hi, I have installed the local galaxy. In galaxy, I noticed that the quality score of fastq datasets have to be converted to sanger format before mapping with bowtie etc. But I did not find that the mapping tools (e.g. bowtie) require the quality score of fastq datasets must be sanger format. Is it necessary to convert fastq to sanger format ? Or, this is just the Galaxy's requirement ? Thanks, ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).