Hi, I have heard Curtis mention that LEfSe is not capable of doing paired Wilcoxan rank sum tests on matched samples. Is there a way to do this by command line in the LEfSe program? Thanks, Leigh Research Fellow Laboratory of Human Carcinogenesis National Cancer Institute 37 Convent Dr., Rm 3068A, MSC 4258 Bethesda, MD. 20892-4258 Phone: 301-496-5835 Fax: 301-496-0497 Email: greathousekl@mail.nih.gov