Hi, I'm using bioblend to populate a library in galaxy. it's work well (bam and vcf). But I have issues with some gff3.gz files. For few of them (over 40 files) I see errors in the log. If I upload that file (that fails with bioblend) it works using the upload tool on the interface. It's auto-detected as "tabular". I can then change datatype to gff3.gz. It works (with a little issue, the file is described as: 1 line, 41,085 comments. That's not true, there is not comments in my file). If I upload that file using the upload tool on the interface, it will fail if I set the datatype to gff3.gz during the upload process. I see then the same type of error (as I see with my bioblend script). I need some ideas, do you have tips ? Maybe some clues to follow... thank you. error type is : Traceback (most recent call last): File "lib/galaxy/jobs/runners/__init__.py", line 510, in _finish_or_resubmit_job job_wrapper.finish(tool_stdout, tool_stderr, exit_code, check_output_detected_state=check_output_detected_state, job_stdout=job_stdout, job_stderr=job_stderr) File "lib/galaxy/jobs/__init__.py", line 1428, in finish self.sa_session.flush() File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/scoping.py", line 162, in do return getattr(self.registry(), name)(*args, **kwargs) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/session.py", line 2446, in flush self._flush(objects) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/session.py", line 2584, in _flush transaction.rollback(_capture_exception=True) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/util/langhelpers.py", line 67, in __exit__ compat.reraise(exc_type, exc_value, exc_tb) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/session.py", line 2544, in _flush flush_context.execute() File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/unitofwork.py", line 416, in execute rec.execute(self) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/unitofwork.py", line 583, in execute uow, File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/persistence.py", line 245, in save_obj insert, File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/orm/persistence.py", line 1116, in _emit_insert_statements statement, params File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/base.py", line 980, in execute return meth(self, multiparams, params) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/sql/elements.py", line 273, in _execute_on_connection return connection._execute_clauseelement(self, multiparams, params) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/base.py", line 1099, in _execute_clauseelement distilled_params, File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/base.py", line 1174, in _execute_context e, util.text_type(statement), parameters, None, None File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/base.py", line 1458, in _handle_dbapi_exception util.raise_from_cause(sqlalchemy_exception, exc_info) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/util/compat.py", line 296, in raise_from_cause reraise(type(exception), exception, tb=exc_tb, cause=cause) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/base.py", line 1171, in _execute_context context = constructor(dialect, self, conn, *args) File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/default.py", line 719, in _init_compiled for key in compiled_params File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/engine/default.py", line 719, in <genexpr> for key in compiled_params File "/softs/bioinfo/galaxy-dev/.venv/lib/python2.7/site-packages/sqlalchemy/sql/type_api.py", line 1189, in process return impl_processor(process_param(value, dialect)) File "lib/galaxy/model/custom_types.py", line 319, in process_bind_param value = json_encoder.encode(value).encode() File "/softs/add-ons/Python-2.7.12/lib/python2.7/json/encoder.py", line 209, in encode chunks = list(chunks) File "/softs/add-ons/Python-2.7.12/lib/python2.7/json/encoder.py", line 434, in _iterencode for chunk in _iterencode_dict(o, _current_indent_level): File "/softs/add-ons/Python-2.7.12/lib/python2.7/json/encoder.py", line 408, in _iterencode_dict for chunk in chunks: File "/softs/add-ons/Python-2.7.12/lib/python2.7/json/encoder.py", line 387, in _iterencode_dict yield _encoder(key) StatementError: (exceptions.UnicodeDecodeError) 'utf8' codec can't decode byte 0xa5 in position 9: invalid start byte [SQL: u'INSERT INTO history_dataset_association_history (history_dataset_association_id, update_time, version, name, extension, metadata, extended_metadata_id) VALUES (%(history_dataset_association_id)s, %(update_time)s, %(version)s, %(name)s, %(extension)s, %(_metadata)s, %(extended_metadata_id)s) RETURNING history_dataset_association_history.id'] [parameters: [{'update_time': datetime.datetime(2019, 8, 26, 7, 53, 29, 457330), 'history_dataset_association_id': 3, 'extension': u'gff3.gz', 'extended_metadata_id': None, '_metadata': {'attribute_types': {'\xc4\xa5v\x10\x04>\xd5\x81U\xa5\\\xc7\xa2\xb3t_d\x07': 'str'}, 'comment_lines': 2528, u'dbkey': u'?', 'delimiter': '\t', 'columns': 9, 'column_types': ['str', 'str', 'str', 'int', 'int', 'float', 'str', 'str', 'str'], 'attributes': 1, 'data_lines': 38558}, 'version': 1, 'name': u'Final_ZmCML247v1a.collinearity.gff.gz'}]] Fred