Hi everyone, I am setting up a galaxy instance in my university server that has 64 cores (4 cpus 16 threads each) and 512GB of memory and I am trying to figure out the best configuration in order to exploit it as best as we can. We are planning to use it for about 100-200 people. So far I followed the instructions in https://wiki.galaxyproject.org/Admin/GetGalaxy https://wiki.galaxyproject.org/Admin/Config/Performance/ProductionServer https://wiki.galaxyproject.org/Admin/Config/ApacheProxy and https://wiki.galaxyproject.org/Admin/Config/Performance/Scaling but I found a little bit confusing the different instructions for Paste based processes and uwsgi. I'm planning to use uwsgi in Apache. So I've been using mysql as the galaxy database and added the following part in galaxy.ini [uwsgi] processes = 12 stats = 127.0.0.1:9191 socket = 127.0.0.1:4001 pythonpath = lib threads = 4 logto = /home/galaxy/uwsgi.log master = True [server:handler0] use = egg:Paste#http port = 8090 use_threadpool = True threadpool_workers = 10 [server:handler1] use = egg:Paste#http port = 8091 use_threadpool = True threadpool_workers = 10 [server:handler2] use = egg:Paste#http port = 8092 use_threadpool = True threadpool_workers = 10 [server:handler3] use = egg:Paste#http port = 8093 use_threadpool = True threadpool_workers = 10 [server:handler4] use = egg:Paste#http port = 8094 use_threadpool = True threadpool_workers = 10 [server:handler5] use = egg:Paste#http port = 8095 use_threadpool = True threadpool_workers = 10 # The port on which to listen. #port = 8090

From a previous post i got that you are using 6 handlers for usegalaxy.org so I figured I'd start with that.

Could you help me configure my apache server? So far I've added the following in the apache configuration file: RewriteEngine on RewriteRule ^(.*) http://localhost:8090$1 [P] RewriteRule ^/galaxy$ /galaxy/ [R] RewriteRule ^/galaxy/static/style/(.*) /home/nate/galaxy-dist/static/june_2007_style/blue/$1 [L] RewriteRule ^/galaxy/static/scripts/(.*) /home/nate/galaxy-dist/static/scripts/packed/$1 [L] RewriteRule ^/galaxy/static/(.*) /home/nate/galaxy-dist/static/$1 [L] RewriteRule ^/galaxy/favicon.ico /home/nate/galaxy-dist/static/favicon.ico [L] RewriteRule ^/galaxy/robots.txt /home/nate/galaxy-dist/static/robots.txt [L] <Location "/galaxy"> Sethandler uwsgi-handler uWSGISocket 127.0.0.1:4001 uWSGImaxVars 512 </Location> But I'm not sure how Apache will proxy the different ports from the handler. In the Apache configuration instructions it said I should use a balancer but in the Scaling instructions it states that this is for standalone paste-based processes. Could you help me out please? Thank you, Makis