Hi, We have installed our own Galaxy server and started NGS analysis but during the mapping procedure using either BWA for illumina or BFAST, no reference genome index is available. We have uploaded the hg19.fa file and put it in the Galaxy-dist/database folder. We modified the BWA_index.loc file (the same for BFAST file) indicating the path to the file. We restart the Galaxy. However, still no reference is available through the NGS: mapping modules?. Is there any way to solve this probably simple problem. Kind regards / Sandrine & Abdelhadi -- Sandrine Imbeaud INSERM, UMR U-674, IUH Université Paris Descartes Génomique Fonctionnelle des tumeurs solides 27 rue Juliette Dodu F75010 Paris, France TEL: +33 (0)1 53 72 51 98 FAX: +33 (0)1 53 72 51 92 MOBILE: +33 (0)6 12 69 80 29 http://www.inserm-u674.net/