error return- PICRUSt

Hello! I am a new user of Galaxy, I believe I am working on the MAIN (although I am not sure). I would like to use the PICRUSt tool on Galaxy through the web. I have created an account, but I did not download Galaxy. I uploaded two .biom files generated in QIIME (see attached). The Greengenes reference database that was used to generate these OTU tables is greengenes/gg_12_10 version. On the web-based PICRUSt tool in Galaxy, I selected the only other option than the current Greengenes database, which is listed as May18 2012. When I try to run normalize_by_copy_number.py I get the following error return for both files: Traceback (most recent call last): File "/net/rcstore02/ifs/rc_labs/galaxy/www/galaxy_hutlab14/galaxy-hutlab14-20150109/tools/picrust_galaxy/scripts/normalize_by_copy_number.py", line 146, in <module> main() File “/net/rcstore02/ifs/rc_labs/galax I am at a loss as to what this error term means. I suspect it is a format issue? But not sure what the issue is or how to fix it. Or that I am using the gg_12_10 version? Cheers Becks Rebecca Prescott, Ph.D University of Hawaii Manoa Department of Microbiology 2538 McCarthy Mall Honolulu, HI 96822

Hi Rebecca, You are almost certainly using the Huttenhower lab Galaxy instance (http://huttenhower.sph.harvard.edu/galaxy/). I've CC's Curtis who can hopefully direct you toward a solution. Thanks, James On Wed, Jun 3, 2015 at 8:05 PM, Rebecca Prescott <rebeccap@hawaii.edu> wrote:
Hello!
I am a new user of Galaxy, I believe I am working on the MAIN (although I am not sure). I would like to use the PICRUSt tool on Galaxy through the web. I have created an account, but I did not download Galaxy.
I uploaded two .biom files generated in QIIME (see attached). The Greengenes reference database that was used to generate these OTU tables is greengenes/gg_12_10 version. On the web-based PICRUSt tool in Galaxy, I selected the only other option than the current Greengenes database, which is listed as May18 2012.
When I try to run normalize_by_copy_number.py I get the following error return for both files:
Traceback (most recent call last): File "/net/rcstore02/ifs/rc_labs/galaxy/www/galaxy_hutlab14/galaxy-hutlab14-20150109/tools/picrust_galaxy/scripts/normalize_by_copy_number.py", line 146, in <module> main() File “/net/rcstore02/ifs/rc_labs/galax
I am at a loss as to what this error term means. I suspect it is a format issue? But not sure what the issue is or how to fix it. Or that I am using the gg_12_10 version?
Cheers Becks
Rebecca Prescott, Ph.D University of Hawaii Manoa Department of Microbiology 2538 McCarthy Mall Honolulu, HI 96822
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participants (2)
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James Taylor
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Rebecca Prescott