Thanks for the response Jen! Answers to your questions below. 1. It should be the newest version of Galaxy 2. We did make production server changes but I'll check the admin part again 3. This might be the problem. I tried to install package_samtools_0_1_19 but it failed, then I installed package_samtools_0_1_18 which succeeded, and lastly I installed bam_to_sam 4. I have not tried the main galaxy instance yet. I did mean the "same" file. I just turned off autoscaling to simplify things. I'll get back to after: unistalling package_samtools_0_118 and bam_to_sam and then just installing bam_to_sam and reviewing the how our production server is set up. Thanks Mike From: Jennifer Jackson <jen@bx.psu.edu<mailto:jen@bx.psu.edu>> Date: Tuesday, December 10, 2013 3:03 PM To: Michael Mason <mmason@benaroyaresearch.org<mailto:mmason@benaroyaresearch.org>>, Galaxy Dev <galaxy-dev@bx.psu.edu<mailto:galaxy-dev@bx.psu.edu>> Subject: inconsistent BAM-to-SAM behavior Hi Mike, I am moving this over to the galaxy-dev@bx.psu.edu<mailto:galaxy-dev@bx.psu.edu> mailing list as it concerns a local install. This is the best way to make contact with others working with the same type of Galaxy. https://wiki.galaxyproject.org/MailingLists A few things to provide more context that will help with troubleshooting: 1 - Are you running the most current version of Galaxy? https://wiki.galaxyproject.org/DevNewsBriefs (Nov 4, 2013) 2 - The local has some of the initial production server changes made? initial install: http://getgalaxy.org next steps are under: https://wiki.galaxyproject.org/Admin in particular: https://wiki.galaxyproject.org/Admin/Config/Performance/ProductionServer 3 - You installed SAMTools from the Tool Shed? The config is correct? And are running the latest version? https://wiki.galaxyproject.org/Tool%20Shed#Installing.2C_maintaining_and_uni... https://wiki.galaxyproject.org/Admin/Config/Tool%20Dependencies https://wiki.galaxyproject.org/Admin/Tools/Tool%20Dependencies 4 - When you run the same dataset(s) through on the public Main Galaxy instance at http://usegalaxy.org, is it successful or does it fail? I wasn't sure if you meant that the same file will fail and then pass, or if some fail and some pass. If you could clarify that, it would help. It could mean the difference between a processing problem (pointing to a config or other issue) and a simple memory problem (where allocating more could help). Please keep all replies on the mailing list with a cc. Thanks! Jen Galaxy team On 12/10/13 12:07 PM, Michael Mason wrote: Hi, I have run some top hap output through the samtools BAM-to-SAM wrapper. It creates the SAM file about 50% of the time. The rest of the time it makes an empty file. In this case if I just rerun it often works. I was wondering if anyone else has seen this behavior. I am using a cloud server and think I might need to change some settings. This causes my workflow to fail for many libraries so I also interested in any workarounds anyone has figured out. Thanks in advance for any help. Cheers Mike -- Jennifer Hillman-Jackson http://galaxyproject.org ________________________________ --CONFIDENTIALITY NOTICE--: The information contained in this email is intended for the exclusive use of the addressee and may contain confidential information. If you are not the intended recipient, you are hereby notified that any form of dissemination of this communication is strictly prohibited. www.benaroyaresearch.org