Hi John, Thanks for your comments. Upload was just a tool that I picked to test my initial install, I hadn't installed any tools into the galaxy instance at that time. I set it up to use a shared directory and it is working fine now. One of the goals for this second Galaxy instance at UGA is to isolate the Galaxy installation directory from the shared (cluster wide) storage. Whenever we have a shared storage issue the Galaxy web interface seems to slowdown considerably. The operating system that is running Galaxy is the latest Ubuntu LTS release (Ubuntu seems to be a lot easier to deal with than CentOS 5.x), our cluster is running CentOS 5.x. I am in the process of figuring out how to install tools and their tool dependencies automatically so that the dependencies are compiled on the CentOS machine rather than the Ubuntu machine. Any suggestions? Thanks, -Raj On 09/25/2015 01:15 PM, John Chilton wrote:

Depending on how you set things up - either Galaxy, Nginx, or Apache are creating a file for the upload that is incoming - in the above case I imagine it is this file - /home/galaxy/wkdir/galaxy/database/tmp/tmpp6j83l. This file is outside of Galaxy's data model for tools and jobs - it is just a free floating file in some ways - so the Pulsar client doesn't know it needs to transfer it or how to modify the job description to handle it. It is kind of a very special case for data source tools.

Stepping back a minute, the upload tool is an odd thing to run with pulsar, it would basically need to send a file to pulsar server, pulsar would run a very lightweight script that wouldn't modify the file at all, and then send the same file back to Galaxy with some metadata. It will really slow down uploads to do them this way I think - if at all possibly you should try to run this tool closer to Galaxy or at least not transfer the file (if it is on a shared directory or something).

Hacking up Galaxy to find these uploaded files would be possible and I think I would merge a PR to implement that, but it is such a particular use case (upload shouldn't probably be running on disk that isn't shared with Galaxy) that I don't think it is worth the effort of implementing this right now.

Is this fair? Can you find some shared disk to use for these paths - /home/galaxy/wkdir/galaxy/database/tmp/tmpp6j83l.

-John

On Thu, Sep 24, 2015 at 9:42 PM, Raj Ayyampalayam<raj76@uga.edu> wrote:

...

Hello,

I am setting up a new instance of Galaxy (based on the latest dev branch on github repo) configured to submit the jobs to a remote cluster via pulsar. I used the usegalaxy playbook to get the configuration parameters for the galaxy job_conf.xml and pulsar app.yml files. I was partially successful in running the jobs via pulsar, the regular tools (analysis tools, etc..) are running OK on the remote cluster. I am having trouble getting the upload tool to run on the the remote cluster.

Here is the error I see in the galaxy error report for the job: It looks like the upload tool running on the remote machine is trying to access a file in the database/tmp area of the galaxy server machine.

Traceback (most recent call last): File "/escratch4/apps/galaxy_scratch/pulsar/files/staging/46/tool_files/upload.py", line 430, in <module> __main__() File "/escratch4/apps/galaxy_scratch/pulsar/files/staging/46/tool_files/upload.py", line 405, in __main__ registry.load_datatypes( root_dir=sys.argv[1], config=sys.argv[2] ) File "/panfs/pstor.storage/home/qbcglab/galaxy_run/galaxy/lib/galaxy/datatypes/registry.py", line 94, in load_datatypes tree = galaxy.util.parse_xml( config ) File "/panfs/pstor.storage/home/qbcglab/galaxy_run/galaxy/lib/galaxy/util/__init__.py", line 178, in parse_xml root = tree.parse( fname, parser=ElementTree.XMLParser( target=DoctypeSafeCallbackTarget() ) ) File "/usr/local/python/2.7.8/lib/python2.7/xml/etree/ElementTree.py", line 647, in parse source = open(source, "rb") IOError: [Errno 2] No such file or directory: '/home/galaxy/wkdir/galaxy/database/tmp/tmpp6j83l'

I've created a Gist with the contents of job_conf.xml, app.yml, file action file, error content athttps://gist.github.com/raj76/7be86f6a56714deef050 .

Any suggestions on how to debug this issue?

Thanks, -Raj

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