details: http://www.bx.psu.edu/hg/galaxy/rev/341d7c1c5c9a changeset: 3124:341d7c1c5c9a user: Kelly Vincent <kpvincent@bx.psu.edu> date: Mon Nov 30 09:05:28 2009 -0500 description: Added usage information, added/updated relevant test files, and got all tests working for Lastz diffstat: test-data/lastz_wrapper_out1.sam | 2 - test-data/lastz_wrapper_out2.tabular | 453 +++++++++++++++++++++++++++++++++++++ tools/sr_mapping/lastz_wrapper.py | 106 +++++--- tools/sr_mapping/lastz_wrapper.xml | 39 +- 4 files changed, 539 insertions(+), 61 deletions(-) diffs (742 lines): diff -r efdb6fb5de34 -r 341d7c1c5c9a test-data/lastz_wrapper_out1.sam --- a/test-data/lastz_wrapper_out1.sam Fri Nov 27 10:53:50 2009 -0500 +++ b/test-data/lastz_wrapper_out1.sam Mon Nov 30 09:05:28 2009 -0500 @@ -1,5 +1,3 @@ -@HD VN:1.0 SO:unsorted -@SQ SN:phiX LN:5386 HWI-EAS91_1_306UPAAXX 16 phiX 4295 255 1H35M * 0 0 CCCCCAACTTGATATTAATAACACTATAGACCACC * HWI-EAS91_1_306UPAAXX 16 phiX 3522 255 36M * 0 0 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC * HWI-EAS91_1_306UPAAXX 0 phiX 229 255 36M * 0 0 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC * diff -r efdb6fb5de34 -r 341d7c1c5c9a test-data/lastz_wrapper_out2.tabular --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/lastz_wrapper_out2.tabular Mon Nov 30 09:05:28 2009 -0500 @@ -0,0 +1,453 @@ +#score name1 strand1 size1 start1 zstart1 end1 length1 text1 name2 strand2 size2 start2 zstart2 end2 start2+ zstart2+ end2+ length2 text2 diff cigar identity idPct coverage covPct gaprate gapPct diagonal shingle +3311 PHIX174 + 5386 4295 4294 4329 35 CCCCCAACTTGATATTAATAACACTATAGACCACC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCCCCAACTTGATATTAATAACACTATAGACCACC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4293 NA +3429 PHIX174 + 5386 3522 3521 3557 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3521 NA +3429 PHIX174 + 5386 229 228 264 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 228 NA +3411 PHIX174 + 5386 2071 2070 2106 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA +3311 PHIX174 + 5386 1067 1066 1101 35 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GGTCAGATTGGTCGTCTTATTACCATTTCAACTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1065 NA +3429 PHIX174 + 5386 1788 1787 1823 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCGTGTTCAACAGACCTATAAACATTCTGTGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1787 NA +3393 PHIX174 + 5386 1193 1192 1228 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1192 NA +3206 PHIX174 + 5386 4563 4562 4598 36 GGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTGGTTTATCGTTTTTGACACTCTCACGTTGTCT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4562 NA +3447 PHIX174 + 5386 2668 2667 2703 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2667 NA +3402 PHIX174 + 5386 4248 4247 4283 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTACTGAGAAGTTAATGGATGAATTGGCACAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4247 NA +3393 PHIX174 + 5386 2863 2862 2898 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2862 NA +3402 PHIX174 + 5386 2557 2556 2592 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2556 NA +3184 PHIX174 + 5386 2511 2510 2544 34 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GTCAACGTTATATTTTGATAGTTTGACGGTTAAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 2510 NA +3420 PHIX174 + 5386 5152 5151 5187 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATGAAAATGCTCACAATGACAAATCTGTCCACGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5151 NA +3402 PHIX174 + 5386 1590 1589 1625 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1589 NA +3456 PHIX174 + 5386 4884 4883 4919 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTACAGTATGCCCATCGCAGTTCGCTACACGCAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4883 NA +3411 PHIX174 + 5386 5052 5051 5087 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5051 NA +3447 PHIX174 + 5386 4909 4908 4944 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4908 NA +3393 PHIX174 + 5386 2863 2862 2898 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGATTGGTTTCGCTGAATCAGGTTATTAAAGAGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2862 NA +3197 PHIX174 + 5386 4173 4172 4208 36 GTTGAGTTCGATAATGGTGATATGTATGTTGACGGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTGAGTTCGATAATGGTGATATGTATGTTTACGGC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4172 NA +3429 PHIX174 + 5386 1701 1700 1736 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1700 NA +3429 PHIX174 + 5386 1683 1682 1718 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATGACGCTGACAACCGTCCTTTACTTGTCATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1682 NA +3365 PHIX174 + 5386 3126 3125 3160 35 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GAGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3124 NA +3420 PHIX174 + 5386 1665 1664 1700 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTTGGAGGTAAAACCTCTTATGACGCTGACAACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1664 NA +3402 PHIX174 + 5386 3213 3212 3248 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3212 NA +3411 PHIX174 + 5386 3420 3419 3455 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGATTGCCGAGATGCAAAATGAGACTCAAAAAGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3419 NA +3465 PHIX174 + 5386 513 512 548 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 512 NA +3179 PHIX174 + 5386 2879 2878 2914 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATAAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC ..x................................. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2878 NA +3438 PHIX174 + 5386 4471 4470 4506 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4470 NA +3393 PHIX174 + 5386 1229 1228 1264 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTTATGGTGAACAGTGGATTAAGTTCATGAAGGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1228 NA +3393 PHIX174 + 5386 615 614 650 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTTTATTGCTGCCGTCATTGCTTATTATGTTCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 614 NA +3429 PHIX174 + 5386 257 256 292 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCACGTTCGTCAAGGACTGGTTTAGATATGAGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 256 NA +3366 PHIX174 + 5386 2381 2380 2416 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2380 NA +3429 PHIX174 + 5386 4098 4097 4133 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4097 NA +3438 PHIX174 + 5386 5099 5098 5134 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGTCGCTACTTCCCAAGAAGCTGTTCAGAATCAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5098 NA +3393 PHIX174 + 5386 4254 4253 4289 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4253 NA +3411 PHIX174 + 5386 3408 3407 3443 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACAATCAGAAAGAGATTGCCGAGATGCAAAATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3407 NA +3429 PHIX174 + 5386 4043 4042 4078 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4042 NA +3411 PHIX174 + 5386 4560 4559 4595 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGGTTGGTTTATCGTTTTTGACACTCTCACGTTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4559 NA +3411 PHIX174 + 5386 1888 1887 1923 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1887 NA +3311 PHIX174 + 5386 3434 3433 3468 35 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCAAAATGAGACTCAAAAAGAGATTGCTGGCATTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3432 NA +3438 PHIX174 + 5386 4539 4538 4574 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAATGCGACAGGCTCATGCTGATGGTTGGTTTATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4538 NA +3420 PHIX174 + 5386 1828 1827 1863 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1827 NA +3420 PHIX174 + 5386 3708 3707 3743 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTTAGTTCATCAGCAAACGCAGAATCAGCGGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3707 NA +3465 PHIX174 + 5386 3326 3325 3361 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3325 NA +3411 PHIX174 + 5386 4176 4175 4211 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTTCGATAATGGTGATATGTATGTTGACGGCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4175 NA +3402 PHIX174 + 5386 22 21 57 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGAAGTTAACACTTTCGGATATTTCTGATGAGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 21 NA +3402 PHIX174 + 5386 2557 2556 2592 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGTTTTCTTCATTGCATTCAGATGGATACATCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2556 NA +3420 PHIX174 + 5386 3277 3276 3312 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3276 NA +3438 PHIX174 + 5386 3592 3591 3627 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3591 NA +3302 PHIX174 + 5386 358 357 392 35 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATGCTGTTCAACCACTAATAGGTAAGAAATCATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 357 NA +3420 PHIX174 + 5386 1775 1774 1810 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1774 NA +3393 PHIX174 + 5386 3504 3503 3539 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATATGCACAAAATGAGATGCTTGCTTATCAACAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3503 NA +3420 PHIX174 + 5386 1554 1553 1589 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGACTTCTACCACATCTATTGACATTATGGGTCTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1553 NA +3447 PHIX174 + 5386 1002 1001 1037 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1001 NA +3420 PHIX174 + 5386 3626 3625 3661 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTTACTCAAGCTCAAACGGCTGGTCAGTATTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3625 NA +3420 PHIX174 + 5386 3572 3571 3607 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3571 NA +3438 PHIX174 + 5386 1265 1264 1300 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTGTTAATGCCACTCCTCTCCCGACTGTTAACACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1264 NA +3411 PHIX174 + 5386 1332 1331 1367 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCACGATTAACCCTGATACCAATAAAATCCCTAAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1331 NA +3393 PHIX174 + 5386 4254 4253 4289 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGAAGTTAATGGATGAATTGGCACAATGCTACAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4253 NA +3411 PHIX174 + 5386 4170 4169 4205 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCGTTGAGTTCGATAATGGTGATATGTATGTTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4169 NA +3465 PHIX174 + 5386 3119 3118 3154 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCCTGATGAGGCCGCCCCTAGTTTTGTTTCTGGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3118 NA +3438 PHIX174 + 5386 1670 1669 1705 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAGGTAAAACCTCTTATGACGCTGACAACCGTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1669 NA +3420 PHIX174 + 5386 640 639 675 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 639 NA +3311 PHIX174 + 5386 2750 2749 2784 35 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTTATTATACCGTCAAGGACTGTGTGACTATTGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2749 NA +3402 PHIX174 + 5386 4606 4605 4641 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GATTAGAGGCGTTTTATGATAATCCCAATGCTTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4605 NA +3420 PHIX174 + 5386 3573 3572 3608 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3572 NA +3420 PHIX174 + 5386 5105 5104 5140 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5104 NA +3420 PHIX174 + 5386 1674 1673 1709 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTAAAACCTCTTATGACGCTGACAACCGTCCTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1673 NA +3438 PHIX174 + 5386 4095 4094 4130 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4094 NA +3411 PHIX174 + 5386 4551 4550 4586 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4550 NA +3411 PHIX174 + 5386 3284 3283 3319 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3283 NA +3402 PHIX174 + 5386 4005 4004 4040 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAATGTCACGCTGATTATTTTGACTTTGAGCGTATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4004 NA +3420 PHIX174 + 5386 2479 2478 2514 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2478 NA +3420 PHIX174 + 5386 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA +3338 PHIX174 + 5386 3045 3044 3079 35 GCTACCGATAACAATACTGTAGGCATGGGTGATGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCTACCGATAACAATACTGTAGGCATGGGTGATGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3043 NA +3492 PHIX174 + 5386 1115 1114 1150 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1114 NA +3206 PHIX174 + 5386 636 635 671 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGTCC .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 635 NA +3060 PHIX174 + 5386 1139 1138 1172 34 GTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GTTGGCGCTCTCCGTCTTTCTCCATTTCGTCGTG ..........................x....... 34M 33/34 97.1% 34/36 94.4% 0/34 0.0% 1138 NA +3447 PHIX174 + 5386 852 851 887 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 851 NA +3429 PHIX174 + 5386 5272 5271 5307 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5271 NA +3127 PHIX174 + 5386 4330 4329 4365 36 GCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCCCGAAGGGGACNANAAATGGTTTTTAGAGAACG ..............X.X................... 36M 34/34 100.0% 36/36 100.0% 0/36 0.0% 4329 NA +3465 PHIX174 + 5386 4891 4890 4926 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGCCCATCGCAGTTCGCTACACGCAGGACGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4890 NA +3429 PHIX174 + 5386 4783 4782 4818 36 AtTTTATGCGGACACTTCCTACAGGTAGCGTTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTTTATGCGGACACTTCCTACAGGTAGCGTTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4782 NA +3438 PHIX174 + 5386 3619 3618 3654 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCAAATGCTTACTCAAGCTCAAACGGCTGGTCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3618 NA +3215 PHIX174 + 5386 4455 4454 4490 36 GGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTATTAAGGATGAGTGTTCAAGATTGCTGGATGCC ................................x... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4454 NA +3393 PHIX174 + 5386 632 631 667 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTGCTTATTATGTTCATCCCGTCAACATTCAAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 631 NA +3347 PHIX174 + 5386 2964 2963 2998 35 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2963 NA +3447 PHIX174 + 5386 2763 2762 2798 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2762 NA +3465 PHIX174 + 5386 2766 2765 2801 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2765 NA +3429 PHIX174 + 5386 1719 1718 1754 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCTAATCTCTGGGCATCTGGCTATGATGTTGATG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1718 NA +3456 PHIX174 + 5386 3100 3099 3135 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3099 NA +3133 PHIX174 + 5386 598 597 632 35 GCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTTTCCTGCTCCTGTTGAGTTTATTGCTTCCGTC .............................x..... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 597 NA +3420 PHIX174 + 5386 4126 4125 4161 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4125 NA +3420 PHIX174 + 5386 4026 4025 4061 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4025 NA +3402 PHIX174 + 5386 2621 2620 2656 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGCTGATATTGCTTTTGATGCCGACCCTAAATTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2620 NA +3411 PHIX174 + 5386 2749 2748 2784 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTATTATACCGTCAAGGACTGTGTGACTATTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2748 NA +3402 PHIX174 + 5386 379 378 414 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTAAGAAATCATGAGTCAAGTTACTGAACAATCCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 378 NA +3438 PHIX174 + 5386 2160 2159 2195 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCACCATGATTATGACCAGTGTTTCCAGTCCGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2159 NA +3393 PHIX174 + 5386 2873 2872 2908 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2872 NA +3365 PHIX174 + 5386 1508 1507 1542 35 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1506 NA +3420 PHIX174 + 5386 3863 3862 3898 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3862 NA +3429 PHIX174 + 5386 5187 5186 5222 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGTGCTTAATCCAACTTACCAAGCTGGGTTACGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5186 NA +3447 PHIX174 + 5386 4196 4195 4231 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTGACGGCCATAAGGCTGCTTCTGACGTTCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4195 NA +3411 PHIX174 + 5386 635 634 670 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGCTTATTATGTTCATCCCGTCAACATTCAAACGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 634 NA +3311 PHIX174 + 5386 1294 1293 1328 35 TAACACTACTGGTTATATTGACCATGCCGCTTTTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TAACACTACTGGTTATATTGACCATGCCGCTTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1292 NA +3420 PHIX174 + 5386 5157 5156 5192 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAAATGCTCACAATGACAAATCTGTCCACGGAGTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5156 NA +3447 PHIX174 + 5386 772 771 807 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGCAGGAAACACTGACGTTCTTACTGACGCAGAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 771 NA +3402 PHIX174 + 5386 1224 1223 1259 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCACGTTTATGGTGAACAGTGGATTAAGTTCATGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1223 NA +3138 PHIX174 + 5386 1544 1543 1576 33 CGCCAAATGACGACTTCTACCACATCTATTGAC HWI-EAS91_1_306UPAAXX - 36 4 3 36 1 0 33 33 CGCCAAATGACGACTTCTACCACATCTATTGAC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 1540 NA +3420 PHIX174 + 5386 4840 4839 4875 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4839 NA +3329 PHIX174 + 5386 204 203 238 35 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATTCTGTCAAAAACTGACGCGTTGGATGAGGAGA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 203 NA +3197 PHIX174 + 5386 2481 2480 2516 36 ACCTGTTTTACAGACACCTAAAGCTACATCGTCAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCTGTTTTAAAGACACCTAAAGCTACATCGTCAAC ..........x......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2480 NA +3429 PHIX174 + 5386 332 331 367 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGAGCGTGGATTACTATCTGAGTCCGATGCTGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 331 NA +3393 PHIX174 + 5386 3978 3977 4013 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTATGGTTCGTTCTTATTACCCTTCTGAATGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3977 NA +3420 PHIX174 + 5386 4865 4864 4900 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4864 NA +3411 PHIX174 + 5386 3271 3270 3306 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAAGGATACTCGTGATTATCTTGCTGCTGCATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3270 NA +3411 PHIX174 + 5386 4174 4173 4209 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4173 NA +3429 PHIX174 + 5386 2738 2737 2773 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCATGATGGTGGTTATTATACCGTCAAGGACTGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2737 NA +3329 PHIX174 + 5386 2703 2702 2737 35 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCCGACTGCCTATGATGTTTATCCTTTGAATGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2701 NA +3456 PHIX174 + 5386 2943 2942 2978 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2942 NA +3411 PHIX174 + 5386 3861 3860 3896 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3860 NA +3429 PHIX174 + 5386 3093 3092 3128 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3092 NA +3420 PHIX174 + 5386 3151 3150 3186 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3150 NA +3393 PHIX174 + 5386 1193 1192 1228 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGACATTTTTACTTTTTATGTCCCTCATCGTCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1192 NA +3456 PHIX174 + 5386 157 156 192 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 156 NA +3429 PHIX174 + 5386 4792 4791 4827 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGACACTTCCTACAGGTAGCGTTGACCCTAATTTTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4791 NA +3402 PHIX174 + 5386 3849 3848 3884 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATACTTGGAACAATTTCTGGAAAGACGGTAAAGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3848 NA +3329 PHIX174 + 5386 2743 2742 2777 35 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GATGGTGGTTATTATACCGTCAAGGACTGTGTGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2741 NA +3465 PHIX174 + 5386 3015 3014 3050 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3014 NA +3438 PHIX174 + 5386 1091 1090 1126 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1090 NA +3329 PHIX174 + 5386 798 797 832 35 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GACGCAGAAGAAAACGTGCGTCAAAAATTACGTGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 796 NA +3420 PHIX174 + 5386 4866 4865 4901 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAAATACGTGGCCTTATGGTTACAGTATGCCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4865 NA +3411 PHIX174 + 5386 4404 4403 4439 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAACGCCCTCTTAAGGATATTCGCGATGAGTATAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4403 NA +3384 PHIX174 + 5386 1655 1654 1690 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTATTTCTTCATTTGGAGGTAAAACCTCTTATGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1654 NA +3420 PHIX174 + 5386 5162 5161 5197 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCACAATGACAAATCTGTCCACGGAGTGCTTAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5161 NA +3320 PHIX174 + 5386 1249 1248 1283 35 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1247 NA +3402 PHIX174 + 5386 3261 3260 3296 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGATAAAGGAAAGGATACTCGTGATTATCTTGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3260 NA +3429 PHIX174 + 5386 3096 3095 3131 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3095 NA +3384 PHIX174 + 5386 2811 2810 2846 36 tGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2810 NA +3465 PHIX174 + 5386 3459 3458 3494 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGCATTCAGTCGGCGACTTCACGCCAGAATACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3458 NA +3320 PHIX174 + 5386 789 788 823 35 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 788 NA +3420 PHIX174 + 5386 3699 3698 3734 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGAGGTTGACTTAGTTCATCAGCAAACGCAGAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3698 NA +3206 PHIX174 + 5386 2641 2640 2676 36 GCCGACCCTAAATTTTTTGCCTGTTTGGTTCGCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCGACCCTAAATTTTTTGCCTGTTTGGTTCTCTTT ...............................x.... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2640 NA +3393 PHIX174 + 5386 305 304 340 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 304 NA +3329 PHIX174 + 5386 3066 3065 3100 35 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GGCATGGGTGATGCTGGTATTAAATCTGCCATTCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3065 NA +3375 PHIX174 + 5386 4428 4427 4463 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGAGTATAATTACCCCAAAAAGAAAGGTATTAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4427 NA +3429 PHIX174 + 5386 1461 1460 1496 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGATGATGCTCGTTATGGTTTCCGTTGCTGCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1460 NA +3402 PHIX174 + 5386 4000 3999 4035 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTCTGAATGTCACGCTGATTATTTTGACTTTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3999 NA +3429 PHIX174 + 5386 220 219 255 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 219 NA +3233 PHIX174 + 5386 3194 3193 3229 36 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTTCT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3193 NA +3124 PHIX174 + 5386 1266 1265 1300 35 GTGTTAATGCCACTCCTCTCCCGACTGTTAACACT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTGTTAATGCCACTCCTCTCCCGACTGTTAACTCT ................................x.. 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 1265 NA +3429 PHIX174 + 5386 4596 4595 4631 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTGACGACCGATTAGAGGCGTTTTATGATAATCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4595 NA +3311 PHIX174 + 5386 1172 1171 1206 35 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GGCCTTGCTATTGACTCTACTGTAGACATTTTTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1170 NA +3420 PHIX174 + 5386 636 635 671 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTTATTATGTTCATCCCGTCAACATTCAAACGGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 635 NA +3393 PHIX174 + 5386 2873 2872 2908 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2872 NA +3474 PHIX174 + 5386 1014 1013 1049 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1013 NA +3438 PHIX174 + 5386 2689 2688 2724 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2688 NA +3206 PHIX174 + 5386 2148 2147 2183 36 GCGTACTTATTCGCCACCATGATTATGACCAGTGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGTACTTATTCGCCACCATGATTATTACCAGTGTT ..........................x......... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2147 NA +3347 PHIX174 + 5386 5191 5190 5225 35 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GCTTAATCCAACTTACCAAGCTGGGTTACGACGCG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 5190 NA +3402 PHIX174 + 5386 1590 1589 1625 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCTGCTTATGCTAATTTGCATACTGACCAAGAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1589 NA +3142 PHIX174 + 5386 571 570 605 35 GTACGCTGGACTTTGTGGGATACCCTCGCTTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTACGCTGTACTTTGTGGGATACCCTCGCTTTCCT ........x.......................... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 570 NA +3438 PHIX174 + 5386 242 241 277 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 241 NA +3429 PHIX174 + 5386 338 337 373 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTGGATTACTATCTGAGTCCGATGCTGTTCAACCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 337 NA +3278 PHIX174 + 5386 1112 1111 1147 36 GCTGGCGACTCCTTCGAGATGGACGCCGTTGGCGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGCGACTCCTTCGAGATGGACGCCGTTTGCGCT ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1111 NA +3247 PHIX174 + 5386 1254 1253 1287 34 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 TCATGAAGGATGGTGTTAATGCCACTCCTCTCCC .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1251 NA +3242 PHIX174 + 5386 2970 2969 3005 36 GCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGGTGGCGCCATGTCTAAATTTTTTGGAGGCGGT .......................x............ 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2969 NA +3420 PHIX174 + 5386 5151 5150 5186 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGGATGAAAATGCTCACAATGACAAATCTGTCCACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5150 NA +3411 PHIX174 + 5386 348 347 383 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATCTGAGTCCGATGCTGTTCAACCACTAATAGGTAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 347 NA +3365 PHIX174 + 5386 4888 4887 4922 35 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4886 NA +3111 PHIX174 + 5386 3972 3971 4004 33 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GGCTTTTTTATGGTTCGTTCTTATTACCCTTCT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 3971 NA +3438 PHIX174 + 5386 5033 5032 5068 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCAGATATGGACCTTGCTGCTAAAGGTCTAGGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5032 NA +3320 PHIX174 + 5386 1552 1551 1586 35 GACGACTTCTACCACATCTATTGACATTATGGGTC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GACGACTTCTACCACATCTATTGACATTATGGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1550 NA +3429 PHIX174 + 5386 1764 1763 1799 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1763 NA +3438 PHIX174 + 5386 1529 1528 1564 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAGACTGAGCTTTCTCGCCAAATGACGACTTCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1528 NA +3438 PHIX174 + 5386 4950 4949 4985 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4949 NA +3447 PHIX174 + 5386 4484 4483 4519 36 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTaC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4483 NA +3429 PHIX174 + 5386 177 176 212 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTTGCGACCTTTCGCCATCAACTAACGATTCTGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 176 NA +3429 PHIX174 + 5386 2577 2576 2612 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GATGGATACATCTGTCAACGCCGCTAATCAGGTTGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2576 NA +3456 PHIX174 + 5386 156 155 191 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 155 NA +3429 PHIX174 + 5386 227 226 262 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 226 NA +3465 PHIX174 + 5386 574 573 609 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 573 NA +3438 PHIX174 + 5386 3021 3020 3056 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTGGCATTCAAGGTGATGTGCTTGCTACCGATAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3020 NA +3438 PHIX174 + 5386 3731 3730 3766 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAATCAGCGGTATGGCTCTTCTCATATTGGCGCTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3730 NA +3420 PHIX174 + 5386 2436 2435 2471 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2435 NA +3411 PHIX174 + 5386 2710 2709 2745 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCCTATGATGTTTATCCTTTGAATGGTCGCCATGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2709 NA +3079 PHIX174 + 5386 4228 4227 4262 35 TTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCGTGATGAGTTTGTATCTGTTACTGATAAGTTA ............................x...... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 4227 NA +3202 PHIX174 + 5386 4606 4605 4639 34 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GATTAGAGGCGTTTTATGATAATCCCAATGCTTT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 4605 NA +3465 PHIX174 + 5386 2766 2765 2801 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGACTGTGTGACTATTGACGTCCTTCCCCGTACGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2765 NA +3256 PHIX174 + 5386 582 581 615 34 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTG .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 581 NA +3474 PHIX174 + 5386 1144 1143 1179 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1143 NA +3465 PHIX174 + 5386 1514 1513 1549 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1513 NA +3393 PHIX174 + 5386 1657 1656 1692 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATTTCTTCATTTGGAGGTAAAACCTCTTATGACGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1656 NA +3411 PHIX174 + 5386 2071 2070 2106 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTATGTTTCTCCTGCTTATCACCTTCTTGAAGGCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2070 NA +3438 PHIX174 + 5386 3250 3249 3285 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3249 NA +3179 PHIX174 + 5386 1982 1981 2017 36 GAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAAATTTCTATGAATGATGTTTTCCGTTCTGGTGAT ..............x..................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1981 NA +3393 PHIX174 + 5386 2217 2216 2252 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGTTAAATTTAATGTGACCGTTTATCGCAATCTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2216 NA +3115 PHIX174 + 5386 3480 3479 3514 35 TCACGCCAGAATACGAAAGACCAGGTATATGCACA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TCACGACAGAATACGAAAGACCAGGTATATGCACA .....x............................. 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 3478 NA +3438 PHIX174 + 5386 1690 1689 1725 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1689 NA +3420 PHIX174 + 5386 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA +3402 PHIX174 + 5386 4282 4281 4317 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4281 NA +3229 PHIX174 + 5386 4136 4135 4169 34 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 GCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAG .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 4135 NA +3384 PHIX174 + 5386 4414 4413 4449 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAAGGATATTCGCGATGAGTATAATTACCCCAAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4413 NA +3393 PHIX174 + 5386 3988 3987 4023 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTCTTATTACCCTTCTGAATGTCACGCTGATTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3987 NA +3456 PHIX174 + 5386 4203 4202 4238 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4202 NA +3215 PHIX174 + 5386 4098 4097 4133 36 ATGCTTGGCTTCCATAAGCAGATGGATAACCGCATC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTTGGCTTCAATAAGCAGATGGATAACCGCATC ............x....................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4097 NA +3338 PHIX174 + 5386 4307 4306 4341 35 TATTAATAACACTATAGACCACCGCCCCGAAGGGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATTAATAACACTATAGACCACCGCCCCGAAGGGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4306 NA +3347 PHIX174 + 5386 1521 1520 1555 35 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1520 NA +3429 PHIX174 + 5386 945 944 980 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 944 NA +3411 PHIX174 + 5386 1575 1574 1610 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1574 NA +3438 PHIX174 + 5386 2689 2688 2724 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCCGACTACCCTCCCGACTGCCTATGATGTTTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2688 NA +3447 PHIX174 + 5386 2099 2098 2134 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GAAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2098 NA +3483 PHIX174 + 5386 524 523 559 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 523 NA +3438 PHIX174 + 5386 1252 1251 1287 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTCATGAAGGATGGTGTTAATGCCACTCCTCTCCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1251 NA +3242 PHIX174 + 5386 4916 4915 4951 36 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGTCC .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4915 NA +3188 PHIX174 + 5386 3828 3827 3863 36 GGTATTGATAAAGCTGTTGCCGATACTTGGAACAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTATTGATAAAGCTGTTGCCGATACTTGTAACAAT .............................x...... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3827 NA +3411 PHIX174 + 5386 3756 3755 3791 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTGGCGCTACTGCAAAGGATATTTCTAATGTCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3755 NA +3492 PHIX174 + 5386 1116 1115 1151 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCGACTCCTTCGAGATGGACGCCGTTGGCGCTCTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1115 NA +3334 PHIX174 + 5386 3099 3098 3134 36 CAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAGGCTCTAATGTTCCTAACCCTGATGGGGCCGCC ............................:....... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3098 NA +3393 PHIX174 + 5386 3082 3081 3117 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3081 NA +3393 PHIX174 + 5386 613 612 648 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 612 NA +3447 PHIX174 + 5386 4936 4935 4971 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4935 NA +3393 PHIX174 + 5386 289 288 324 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCACATTTTGTTCATGGTAGAGATTCTCTTGTTGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 288 NA +3438 PHIX174 + 5386 4690 4689 4725 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGGCTAATGATTCACACGCCGACTGCTATCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4689 NA +3420 PHIX174 + 5386 3520 3519 3555 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3519 NA +3393 PHIX174 + 5386 3858 3857 3893 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AACAATTTCTGGAAAGACGGTAAAGCTGATGGTATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3857 NA +3438 PHIX174 + 5386 3344 3343 3379 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3343 NA +3019 PHIX174 + 5386 4672 4671 4707 36 TTCTTGCTGCCGAGGGTCGCAAGGCTAATGATTCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTTGCTGCCGAGGGTCGCAAGGCTATTGTTTCAC ...........................x..x..... 36M 34/36 94.4% 36/36 100.0% 0/36 0.0% 4671 NA +3411 PHIX174 + 5386 1800 1799 1835 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGACCTATAAACATTCTGTGCCGCGTTTCTTTGTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1799 NA +3420 PHIX174 + 5386 5077 5076 5112 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGAACAACTCACTAAAAACCAAGCTGTCGCTACTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5076 NA +3347 PHIX174 + 5386 2763 2762 2797 35 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2761 NA +3402 PHIX174 + 5386 4282 4281 4317 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTACAATGTGCTCCCCCAACTTGATATTAATAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4281 NA +3188 PHIX174 + 5386 1203 1202 1238 36 TTACTTTTTATGTCCCTCATCGTCACGTTTATGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTACTTTTTATGTCCCTCATCGTCACGTTTATGTTG .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1202 NA +3420 PHIX174 + 5386 5107 5106 5142 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5106 NA +3320 PHIX174 + 5386 5168 5167 5202 35 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 AATGACAAATCTGTCCACGGAGTGCTTAATCCAAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 5166 NA +3224 PHIX174 + 5386 2956 2955 2991 36 TTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGCTTCTGCTCTTGCTTGTGGCGCCATGTCTAAAT .................x.................. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2955 NA +3215 PHIX174 + 5386 624 623 659 36 GCTGCCGTCATTGCTTATTATGTTCATCCCGTCAAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTGCCGTCATTGCTTATTATGTTCATCCCTTCAAC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 623 NA +3329 PHIX174 + 5386 1877 1876 1911 35 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCGCCTACTGCGACTAAAGAGATTCAGTACCTTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1875 NA +3429 PHIX174 + 5386 1214 1213 1249 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCCTCATCGTCACGTTTATGGTGAACAGTGGATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1213 NA +3010 PHIX174 + 5386 558 557 593 36 GCTTGCGTTTATGGTACGCTGGACTTTGTGGGATAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTGCGTTTATGGTACGCTGGACTTTTTGTGATAC ...........................x..x..... 36M 34/36 94.4% 36/36 100.0% 0/36 0.0% 557 NA +3411 PHIX174 + 5386 3629 3628 3664 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TACTCAAGCTCAAACGGCTGGTCAGTATTTTACCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3628 NA +3320 PHIX174 + 5386 2254 2253 2288 35 GACCACTCGCGATTCAATCATGACTTCGTGATAAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GACCACTCGCGATTCAATCATGACTTCGTGATAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2253 NA +3420 PHIX174 + 5386 2479 2478 2514 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCACCTGTTTTACAGACACCTAAAGCTACATCGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2478 NA +3474 PHIX174 + 5386 3001 3000 3036 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCGGTCAAAAAGCCGCCTCCGGTGGCATTCAAGGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3000 NA +3356 PHIX174 + 5386 4667 4666 4701 35 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATGGTTCTTGCTGCCGAGGGTCGCAAGGCTAATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4666 NA +3438 PHIX174 + 5386 3464 3463 3499 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3463 NA +3338 PHIX174 + 5386 4911 4910 4945 35 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TACACGCAGGACGCTTTTTCACGTTCTGGTTGGTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4910 NA +3197 PHIX174 + 5386 2222 2221 2257 36 AAATTTAATGTGACCGTTTATCGCAATCTGCCGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAATTTAATGTGAACGTTTATCGCAATCTGCCGACC .............x...................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2221 NA +3338 PHIX174 + 5386 646 645 680 35 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCATCCCGTCAACATTCAAACGGCCTGTCTCATC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 645 NA +3366 PHIX174 + 5386 1364 1363 1399 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGCATTTGTTTCAGGGTTATTTGAATATCTATAAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1363 NA +3320 PHIX174 + 5386 1613 1612 1647 35 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACTGACCAAGAACGTGATTACTTCATGCAGCGTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1611 NA +3347 PHIX174 + 5386 1045 1044 1079 35 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1044 NA +3411 PHIX174 + 5386 1588 1587 1623 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1587 NA +3420 PHIX174 + 5386 339 338 374 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGATTACTATCTGAGTCCGATGCTGTTCAACCACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 338 NA +3447 PHIX174 + 5386 4937 4936 4972 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4936 NA +3202 PHIX174 + 5386 4344 4343 4377 34 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA HWI-EAS91_1_306UPAAXX - 36 3 2 36 1 0 34 34 GAAAAATGGTTTTTAGAGAACGAGAAGACGGTTA .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 4341 NA +3420 PHIX174 + 5386 3674 3673 3709 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3673 NA +3438 PHIX174 + 5386 4945 4944 4980 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4944 NA +3474 PHIX174 + 5386 1946 1945 1981 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1945 NA +3215 PHIX174 + 5386 2662 2661 2697 36 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCTACT ................................x... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 2661 NA +3447 PHIX174 + 5386 3313 3312 3348 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3312 NA +3438 PHIX174 + 5386 727 726 762 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 726 NA +3447 PHIX174 + 5386 555 554 590 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGGCTTGCGTTTATGGTACGCTGGACTTTGTGGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 554 NA +3384 PHIX174 + 5386 485 484 520 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGATTTCGATTTTCTGACGAGTAACAAAGTTTGGAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 484 NA +3384 PHIX174 + 5386 318 317 353 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTGACATTTTAAAAGAGCGTGGATTACTATCTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 317 NA +3429 PHIX174 + 5386 910 909 945 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 909 NA +3447 PHIX174 + 5386 4936 4935 4971 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4935 NA +3375 PHIX174 + 5386 5005 5004 5040 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5004 NA +3143 PHIX174 + 5386 1379 1378 1414 36 GGTTATTTGAATATCTATAACAACTATTTTAAAGCG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTTATTTGAATATCTATAACAACTATTTTAAATCG .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1378 NA +3233 PHIX174 + 5386 3168 3167 3203 36 GGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GGTAAAGGACTTCTTGAAGGTACGTTGCAGTCTGGC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3167 NA +3138 PHIX174 + 5386 2738 2737 2770 33 GCCATGATGGTGGTTATTATACCGTCAAGGACT HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 GCCATGATGGTGGTTATTATACCGTCAAGGACT ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 2737 NA +3429 PHIX174 + 5386 4099 4098 4134 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCTTGGCTTCCATAAGCAGATGGATAACCGCATCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4098 NA +3429 PHIX174 + 5386 2574 2573 2609 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAGATGGATACATCTGTCAACGCCGCTAATCAGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2573 NA +3429 PHIX174 + 5386 2453 2452 2488 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2452 NA +3438 PHIX174 + 5386 5179 5178 5214 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTCCACGGAGTGCTTAATCCAACTTACCAAGCTGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5178 NA +3015 PHIX174 + 5386 3778 3777 3811 34 TTTCTAATGTCGTCACTGATGCTGCTTCTGGTGT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTTCTAATGTCGTCACTGATGCTGCTTCTGTTGT ..............................x... 34M 33/34 97.1% 34/36 94.4% 0/34 0.0% 3777 NA +3420 PHIX174 + 5386 1843 1842 1878 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1842 NA +3420 PHIX174 + 5386 1082 1081 1117 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTATTACCATTTCAACTACTCCGGTTATCGCTGGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1081 NA +3438 PHIX174 + 5386 1728 1727 1763 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1727 NA +3188 PHIX174 + 5386 4637 4636 4672 36 TTTGCGTGACTATTTTCGTGATATTGGTCGTATGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTGCGTGACTATTTTCGTGATATTGTTCGTATGGT ..........................x......... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4636 NA +3420 PHIX174 + 5386 2225 2224 2260 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTAATGTGACCGTTTATCGCAATCTGCCGACCACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2224 NA +3384 PHIX174 + 5386 3368 3367 3403 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3367 NA +3456 PHIX174 + 5386 3311 3310 3346 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3310 NA +3420 PHIX174 + 5386 5039 5038 5074 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5038 NA +3420 PHIX174 + 5386 2059 2058 2094 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2058 NA +3411 PHIX174 + 5386 388 387 423 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCATGAGTCAAGTTACTGAACAATCCGTACGTTTCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 387 NA +3420 PHIX174 + 5386 2754 2753 2789 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATACCGTCAAGGACTGTGTGACTATTGACGTCCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2753 NA +3420 PHIX174 + 5386 2128 2127 2163 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2127 NA +3438 PHIX174 + 5386 154 153 189 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 153 NA +3447 PHIX174 + 5386 3617 3616 3652 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCGCCAAATGCTTACTCAAGCTCAAACGGCTGGTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3616 NA +3420 PHIX174 + 5386 2728 2727 2763 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2727 NA +3456 PHIX174 + 5386 2775 2774 2810 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GACTATTGACGTCCTTCCCCGTACGCCGGGCAATAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2774 NA +3411 PHIX174 + 5386 4217 4216 4252 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4216 NA +3293 PHIX174 + 5386 4619 4618 4653 35 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTATGATAATCCCAATGCTTTGCGTGACTATTTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4618 NA +3338 PHIX174 + 5386 411 410 445 35 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 410 NA +3329 PHIX174 + 5386 2730 2729 2764 35 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GAATGGTCGCCATGATGGTGGTTATTATACCGTCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2729 NA +3383 PHIX174 + 5386 521 520 555 35 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 520 NA +3402 PHIX174 + 5386 4260 4259 4295 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAATGGATGAATTGGCACAATGCTACAATGTGCTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4259 NA +3411 PHIX174 + 5386 1662 1661 1697 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTTCATTTGGAGGTAAAACCTCTTATGACGCTGACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1661 NA +3465 PHIX174 + 5386 533 532 568 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTCTCGTGCTCGTCGCTGCGTTGAGGCTTGCGTTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 532 NA +3320 PHIX174 + 5386 195 194 229 35 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCAACTAACGATTCTGTCAAAAACTGACGCGTTGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 194 NA +3429 PHIX174 + 5386 2239 2238 2274 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATCGCAATCTGCCGACCACTCGCGATTCAATCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2238 NA +3224 PHIX174 + 5386 1313 1312 1348 36 GACCATGCCGCTTTTCTTGGCACGATTAACCCTGAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GACCATGCCGCTTTTCTTGGCACGATTAACCCTTAT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1312 NA +3402 PHIX174 + 5386 1545 1544 1580 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCCAAATGACGACTTCTACCACATCTATTGACATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1544 NA +3438 PHIX174 + 5386 557 556 592 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGCTTGCGTTTATGGTACGCTGGACTTTGTGGGATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 556 NA +3411 PHIX174 + 5386 1249 1248 1284 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAGTTCATGAAGGATGGTGTTAATGCCACTCCTCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1248 NA +3197 PHIX174 + 5386 4174 4173 4209 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTGAGTTCGATAATGGTGATATGTATGTTGACGTCC .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4173 NA +3302 PHIX174 + 5386 614 613 648 35 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGAGTTTATTGCTGCCGTCATTGCTTATTATGTTC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 613 NA +3420 PHIX174 + 5386 206 205 241 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGTCAAAAACTGACGCGTTGGATGAGGAGAAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 205 NA +3411 PHIX174 + 5386 4981 4980 5016 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4980 NA +3215 PHIX174 + 5386 1702 1701 1737 36 TCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCTTTACTTGTCATGCGCTCTAATCTCTGTGCATC ..............................x..... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 1701 NA +3302 PHIX174 + 5386 2321 2320 2355 35 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2320 NA +3338 PHIX174 + 5386 4088 4087 4122 35 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TCAATCCCCAATGCTTGGCTTCCATAAGCAGATGG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4087 NA +3456 PHIX174 + 5386 900 899 935 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 899 NA +3429 PHIX174 + 5386 1155 1154 1190 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1154 NA +3438 PHIX174 + 5386 3587 3586 3622 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3586 NA +3420 PHIX174 + 5386 3787 3786 3822 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3786 NA +3311 PHIX174 + 5386 3834 3833 3868 35 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GATAAAGCTGTTGCCGATACTTGGAACAATTTCTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3833 NA +3393 PHIX174 + 5386 989 988 1024 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGAGGATAAATTATGTCTAATATTCAAACTGGCGCC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 988 NA +3429 PHIX174 + 5386 3712 3711 3747 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3711 NA +3402 PHIX174 + 5386 1207 1206 1242 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTTTATGTCCCTCATCGTCACGTTTATGGTGAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1206 NA +3447 PHIX174 + 5386 2116 2115 2151 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2115 NA +3438 PHIX174 + 5386 660 659 695 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 659 NA +3420 PHIX174 + 5386 1536 1535 1571 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGCTTTCTCGCCAAATGACGACTTCTACCACATCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1535 NA +3329 PHIX174 + 5386 2112 2111 2146 35 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2110 NA +3402 PHIX174 + 5386 2816 2815 2851 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2815 NA +3429 PHIX174 + 5386 5176 5175 5211 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATCTGTCCACGGAGTGCTTAATCCAACTTACCAAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5175 NA +3447 PHIX174 + 5386 3472 3471 3507 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGGCGACTTCACGCCAGAATACGAAAGACCAGGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3471 NA +3465 PHIX174 + 5386 1948 1947 1983 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGACCCTGTTTTGTATGGCAACTTGCCGCCGCGTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1947 NA +3293 PHIX174 + 5386 2212 2211 2246 35 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TAGTCAGGTTAAATTTAATGTGACCGTTTATCGCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2211 NA +3284 PHIX174 + 5386 3817 3816 3851 35 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 ATATTTTTCATGGTATTGATAAAGCTGTTGCCGAT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3816 NA +3393 PHIX174 + 5386 3563 3562 3598 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTCTATTATGGAAAACACCAATCTTTCCAAGCAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3562 NA +3347 PHIX174 + 5386 4198 4197 4232 35 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATGTTGACGGCCATAAGGCTGCTTCTGACGTTCGT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4196 NA +3329 PHIX174 + 5386 2907 2906 2941 35 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCAGCCACTTAAGTGAGGTGATTTATGTTTGGTGC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2905 NA +3347 PHIX174 + 5386 2843 2842 2877 35 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTACCGCTACTAAATGCCGCGGATTGGTTTCGCTG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2842 NA +3251 PHIX174 + 5386 4374 4373 4409 36 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTTACGCAGTTTTGCCGCAAGCTGGCTGCTGTACGC ...............................x.... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4373 NA +3347 PHIX174 + 5386 1940 1939 1974 35 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1938 NA +3411 PHIX174 + 5386 5073 5072 5108 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAATGGAACAACTCACTAAAAACCAAGCTGTCGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5072 NA +3375 PHIX174 + 5386 4434 4433 4469 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TATAATTACCCCAAAAAGAAAGGTATTAAGGATGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4433 NA +3347 PHIX174 + 5386 1274 1273 1308 35 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCCACTCCTCTCCCGACTGTTAACACTACTGGTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1272 NA +3393 PHIX174 + 5386 4440 4439 4475 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4439 NA +3411 PHIX174 + 5386 3920 3919 3955 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GTCAGGATTGACACCCTCCCAATTGTATGTTTTCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3919 NA +3347 PHIX174 + 5386 4587 4586 4621 35 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CTCACGTTGGCTGACGACCGATTAGAGGCGTTTTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4585 NA +3438 PHIX174 + 5386 1707 1706 1742 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1706 NA +3420 PHIX174 + 5386 1582 1581 1617 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GGGTCTGCAAGCTGCTTATGCTAATTTGCATACTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1581 NA +3411 PHIX174 + 5386 1588 1587 1623 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCAAGCTGCTTATGCTAATTTGCATACTGACCAAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1587 NA +3166 PHIX174 + 5386 1180 1179 1213 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1179 NA +3420 PHIX174 + 5386 2698 2697 2733 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCCTCCCGACTGCCTATGATGTTTATCCTTTGAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2697 NA +3438 PHIX174 + 5386 3167 3166 3202 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3166 NA +3420 PHIX174 + 5386 2289 2288 2324 36 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGATTGAGTGTGAGGTTATAACGCCGAAGCGGTAAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2288 NA +3402 PHIX174 + 5386 441 440 476 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 440 NA +3465 PHIX174 + 5386 1012 1011 1047 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1011 NA +3447 PHIX174 + 5386 161 160 196 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 GCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 160 NA +3347 PHIX174 + 5386 1165 1164 1199 35 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCGTCGTGGCCTTGCTATTGACTCTACTGTAGACA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1163 NA +3179 PHIX174 + 5386 5010 5009 5045 36 GTGGCTAAATACGTTAACAAAAAGTCAGATATGGAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTGGATAAATACGTTAACAAAAAGTCAGATATGGAC ....x............................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 5009 NA +3420 PHIX174 + 5386 2286 2285 2321 36 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAAAGATTGAGTGTGAGGTTATAACGCCGAAGCGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2285 NA +3411 PHIX174 + 5386 801 800 836 36 GCAGAAGAAAACGTGCGTCAAAAATTACGTGCaGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 800 NA +3411 PHIX174 + 5386 1587 1586 1622 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGCAAGCTGCTTATGCTAATTTGCATACTGACCAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1586 NA +3438 PHIX174 + 5386 4578 4577 4613 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTTGACACTCTCACGTTGGCTGACGACCGATTAGAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4577 NA +3411 PHIX174 + 5386 3570 3569 3605 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3569 NA +3411 PHIX174 + 5386 496 495 531 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTCTGACGAGTAACAAAGTTTGGATTGCTACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 495 NA +3402 PHIX174 + 5386 441 440 476 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 440 NA +3293 PHIX174 + 5386 4235 4234 4269 35 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGAGTTTGTATCTGTTACTGAGAAGTTAATGGATG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4234 NA +3411 PHIX174 + 5386 2293 2292 2328 36 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGAGTGTGAGGTTATAACGCCGAAGCGGTAAAAATT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2292 NA +3311 PHIX174 + 5386 1579 1578 1613 35 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1577 NA +3242 PHIX174 + 5386 3332 3331 3367 36 TGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTGCTGATGCTTCCTCTGCTGGTATGGTTTACGC ...............................x.... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3331 NA +3411 PHIX174 + 5386 4132 4131 4167 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4131 NA +3029 PHIX174 + 5386 1 0 32 32 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA HWI-EAS91_1_306UPAAXX - 36 5 4 36 1 0 32 32 GAGTTTTATCGCTTCCATGACGCAGAAGTTAA ................................ 32M 32/32 100.0% 32/36 88.9% 0/32 0.0% -4 -32 +3366 PHIX174 + 5386 54 53 89 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTCGAAAAATTATCTTGATAAAGCAGGAATTACTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 53 NA +3302 PHIX174 + 5386 680 679 714 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 678 NA +3429 PHIX174 + 5386 231 230 266 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 230 NA +3429 PHIX174 + 5386 4192 4191 4227 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATATGTATGTTGACGGCCATAAGGCTGCTTCTGACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4191 NA +3411 PHIX174 + 5386 1580 1579 1615 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGGGTCTGCAAGCTGCTTATGCTAATTTGCATACT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1579 NA +3393 PHIX174 + 5386 611 610 646 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTGAGTTTATTGCTGCCGTCATTGCTTATTATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 610 NA +3338 PHIX174 + 5386 4532 4531 4566 35 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GATGAATGCAATGCGACAGGCTCATGCTGATGGTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4530 NA +3097 PHIX174 + 5386 638 637 672 35 TTATTATGTTCATCCCGTCAACATTCAAACGGCCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTATTATGTTCATCCCGTCAACATTCAAACTGCCT ..............................x.... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 637 NA +3420 PHIX174 + 5386 1957 1956 1992 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTGTATGGCAACTTGCCGCCGCGTGAAATTTCTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1956 NA +3420 PHIX174 + 5386 2728 2727 2763 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTGAATGGTCGCCATGATGGTGGTTATTATACCGTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2727 NA +3402 PHIX174 + 5386 3979 3978 4014 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATGGTTCGTTCTTATTACCCTTCTGAATGTCACG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3978 NA +3365 PHIX174 + 5386 540 539 574 35 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 GCTCGTCGCTGCGTTGAGGCTTGCGTTTATGGTAC ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 538 NA +3438 PHIX174 + 5386 1852 1851 1887 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1851 NA +3456 PHIX174 + 5386 4684 4683 4719 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGGTCGCAAGGCTAATGATTCACACGCCGACTGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4683 NA +3197 PHIX174 + 5386 3690 3689 3725 36 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCAGCAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AAGGTTAGTGCTGAGGTTGACTTAGTTCATCATCAA ................................x... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3689 NA +3447 PHIX174 + 5386 584 583 619 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 583 NA +3311 PHIX174 + 5386 3852 3851 3886 35 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3850 NA +3329 PHIX174 + 5386 2857 2856 2891 35 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2856 NA +3438 PHIX174 + 5386 1728 1727 1763 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTGGGCATCTGGCTATGATGTTGATGGAACTGACC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1727 NA +3356 PHIX174 + 5386 4658 4657 4692 35 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4657 NA +3420 PHIX174 + 5386 1721 1720 1756 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCTAATCTCTGGGCATCTGGCTATGATGTTGATGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1720 NA +3429 PHIX174 + 5386 1954 1953 1989 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTTTTGTATGGCAACTTGCCGCCGCGTGAAATTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1953 NA +3438 PHIX174 + 5386 962 961 997 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 961 NA +3438 PHIX174 + 5386 2175 2174 2210 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2174 NA +3420 PHIX174 + 5386 2442 2441 2477 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAGCTGGTTCTCACTTCTGTTACTCCAGCTTCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2441 NA +3329 PHIX174 + 5386 5126 5125 5160 35 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CAGAATCAGAATGAGCCGCAACTTCGGGATGAAAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 5124 NA +3474 PHIX174 + 5386 5206 5205 5241 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 5205 NA +3393 PHIX174 + 5386 2879 2878 2914 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2878 NA +3402 PHIX174 + 5386 2004 2003 2039 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2003 NA +3411 PHIX174 + 5386 2154 2153 2189 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTATTCGCCACCATGATTATGACCAGTGTTTCCAGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2153 NA +3356 PHIX174 + 5386 1093 1092 1127 35 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TTCAACTACTCCGGTTATCGCTGGCGACTCCTTCG ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 1092 NA +3447 PHIX174 + 5386 3127 3126 3162 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGCCGCCCCTAGTTTTGTTTCTGGTGCTATGGCTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3126 NA +3420 PHIX174 + 5386 3544 3543 3579 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTCTACTGCTCGCGTTGCGTCTATTATGGAAAACA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3543 NA +3070 PHIX174 + 5386 2508 2507 2542 35 ATCGTCAACGTTATATTTTGATAGTTTGACGGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 ATCGTCAACGTTATATTTTGATAGTTTGACGTTTA ...............................x... 35M 34/35 97.1% 35/36 97.2% 0/35 0.0% 2507 NA +3420 PHIX174 + 5386 3600 3599 3635 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3599 NA +3420 PHIX174 + 5386 1734 1733 1769 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATCTGGCTATGATGTTGATGGAACTGACCAAACGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1733 NA +3129 PHIX174 + 5386 2655 2654 2687 33 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 33 1 0 33 33 TTTTGCCTGTTTGGTTCGCTTTGAGTCTTCTTC ................................. 33M 33/33 100.0% 33/36 91.7% 0/33 0.0% 2654 NA +3429 PHIX174 + 5386 2934 2933 2969 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2933 NA +3447 PHIX174 + 5386 1522 1521 1557 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1521 NA +3420 PHIX174 + 5386 4063 4062 4098 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4062 NA +3402 PHIX174 + 5386 3395 3394 3430 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3394 NA +3429 PHIX174 + 5386 409 408 444 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 408 NA +3420 PHIX174 + 5386 1867 1866 1902 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1866 NA +3215 PHIX174 + 5386 3780 3779 3815 36 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTAATGTCGTCACTGATGCTGCTTCTGGTGTGTTT .................................x.. 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 3779 NA +3420 PHIX174 + 5386 3982 3981 4017 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3981 NA +3402 PHIX174 + 5386 2815 2814 2850 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2814 NA +3402 PHIX174 + 5386 1242 1241 1277 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1241 NA +3356 PHIX174 + 5386 4659 4658 4693 35 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 ATTGGTCGTATGGTTCTTGCTGCCGAGGGTCGCAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 4657 NA +3420 PHIX174 + 5386 2436 2435 2471 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTCTGATAAGCTGGTTCTCACTTCTGTTACTCCAGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2435 NA +3383 PHIX174 + 5386 527 526 561 35 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 TGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCTT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 526 NA +3260 PHIX174 + 5386 4817 4816 4852 36 CCCTAATTTTGGTCGTCGGGTACGCAATCGCCGCCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CCCTAATTTGGGTCGTCGGGTACGCAATCGCCGCCA .........x.......................... 36M 35/36 97.2% 36/36 100.0% 0/36 0.0% 4816 NA +3447 PHIX174 + 5386 2100 2099 2135 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 AAGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2099 NA +3438 PHIX174 + 5386 2762 2761 2797 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2761 NA +3420 PHIX174 + 5386 4955 4954 4990 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4954 NA +3411 PHIX174 + 5386 3761 3760 3796 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCTACTGCAAAGGATATTTCTAATGTCGTCACTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3760 NA +3438 PHIX174 + 5386 4039 4038 4074 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4038 NA +3438 PHIX174 + 5386 4788 4787 4823 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCGGACACTTCCTACAGGTAGCGTTGACCCTAAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4787 NA +3447 PHIX174 + 5386 1002 1001 1037 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1001 NA +3456 PHIX174 + 5386 4381 4380 4416 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4380 NA +3393 PHIX174 + 5386 4256 4255 4291 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAAGTTAATGGATGAATTGGCACAATGCTACAATGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4255 NA +3329 PHIX174 + 5386 10 9 44 35 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CGCTTCCATGACGCAGAAGTTAACACTTTCGGATA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 8 NA +3411 PHIX174 + 5386 1579 1578 1614 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TATGGGTCTGCAAGCTGCTTATGCTAATTTGCATAC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1578 NA +3411 PHIX174 + 5386 3862 3861 3897 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3861 NA +3420 PHIX174 + 5386 3278 3277 3313 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3277 NA +3420 PHIX174 + 5386 349 348 384 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TCTGAGTCCGATGCTGTTCAACCACTAATAGGTAAG .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 348 NA +3402 PHIX174 + 5386 4608 4607 4643 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TTAGAGGCGTTTTATGATAATCCCAATGCTTTGCGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4607 NA +3284 PHIX174 + 5386 3808 3807 3842 35 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT HWI-EAS91_1_306UPAAXX + 36 1 0 35 1 0 35 35 GTGTGGTTGATATTTTTCATGGTATTGATAAAGCT ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 3807 NA +3393 PHIX174 + 5386 286 285 321 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 285 NA +3429 PHIX174 + 5386 2850 2849 2885 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 TACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2849 NA +3302 PHIX174 + 5386 680 679 714 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CATGGAAGGCGCTGAATTTACGGAAAACATTATTA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 678 NA +3420 PHIX174 + 5386 4740 4739 4775 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 GAGTATGGTACAGCTAATGGCCGTCTTCATTTCCAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4739 NA +3338 PHIX174 + 5386 2467 2466 2501 35 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA HWI-EAS91_1_306UPAAXX - 36 2 1 36 1 0 35 35 CCAGCTTCTTCGGCACCTGTTTTACAGACACCTAA ................................... 35M 35/35 100.0% 35/36 97.2% 0/35 0.0% 2465 NA +3193 PHIX174 + 5386 320 319 353 34 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TTGACATTTTAAAAGAGCGTGGATTACTATCTGA .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 319 NA +3166 PHIX174 + 5386 1180 1179 1213 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT HWI-EAS91_1_306UPAAXX + 36 1 0 34 1 0 34 34 TATTGACTCTACTGTAGACATTTTTACTTTTTAT .................................. 34M 34/34 100.0% 34/36 94.4% 0/34 0.0% 1179 NA +3474 PHIX174 + 5386 521 520 556 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 520 NA +3429 PHIX174 + 5386 333 332 368 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AGAGCGTGGATTACTATCTGAGTCCGATGCTGTTCA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 332 NA +3429 PHIX174 + 5386 2756 2755 2791 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC HWI-EAS91_1_306UPAAXX + 36 1 0 36 1 0 36 36 ATACCGTCAAGGACTGTGTGACTATTGACGTCCTTC .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2755 NA +3411 PHIX174 + 5386 4957 4956 4992 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTATA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4956 NA +3375 PHIX174 + 5386 72 71 107 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 ATAAAGCAGGAATTACTACTGCTTGTTTACGAATTA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 71 NA +3393 PHIX174 + 5386 4249 4248 4284 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 TTACTGAGAAGTTAATGGATGAATTGGCACAATGCT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4248 NA +3438 PHIX174 + 5386 2592 2591 2627 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 2591 NA +3420 PHIX174 + 5386 1974 1973 2009 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 1973 NA +3429 PHIX174 + 5386 3540 3539 3575 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 AAGGAGTCTACTGCTCGCGTTGCGTCTATTATGGAA .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 3539 NA +3402 PHIX174 + 5386 4130 4129 4165 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT HWI-EAS91_1_306UPAAXX - 36 1 0 36 1 0 36 36 CATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTAT .................................... 36M 36/36 100.0% 36/36 100.0% 0/36 0.0% 4129 NA diff -r efdb6fb5de34 -r 341d7c1c5c9a tools/sr_mapping/lastz_wrapper.py --- a/tools/sr_mapping/lastz_wrapper.py Fri Nov 27 10:53:50 2009 -0500 +++ b/tools/sr_mapping/lastz_wrapper.py Mon Nov 30 09:05:28 2009 -0500 @@ -1,6 +1,35 @@ #! /usr/bin/python """ +Runs Lastz +Written for Lastz v. 1.01.86. + +usage: lastz_wrapper.py [options] + --ref_name: The reference name to append to all output matches + --ref_source: Whether the reference is cached or from the history + --source_select: Whether to used pre-set or cached reference file + --input1: The name of the reference file if using history or reference base name if using cached + --input2: The reads file to align + --ref_sequences: The number of sequences in the reference file if using one from history + --pre_set_options: Which of the pre set options to use, if using pre-sets + --strand: Which strand of the read to search, if specifying all parameters + --seed: Seeding settings, if specifying all parameters + --gfextend: Whether to perform gap-free extension of seed hits to HSPs (high scoring segment pairs), if specifying all parameters + --chain: Whether to perform chaining of HSPs, if specifying all parameters + --transition: Number of transitions to allow in each seed hit, if specifying all parameters + --O: Gap opening penalty, if specifying all parameters + --E: Gap extension penalty, if specifying all parameters + --X: X-drop threshold, if specifying all parameters + --Y: Y-drop threshold, if specifying all parameters + --K: Threshold for HSPs, if specifying all parameters + --L: Threshold for gapped alignments, if specifying all parameters + --entropy: Whether to involve entropy when filtering HSPs, if specifying all parameters + --identity_min: Minimum identity (don't report matches under this identity) + --identity_max: Maximum identity (don't report matches above this identity) + --coverage: The minimum coverage value (don't report matches covering less than this) + --out_format: The format of the output file (sam, diffs, or tabular (general)) + --output: The name of the output file + --num_threads: The number of threads to run --lastzSeqsFileDir: Directory of local lastz_seqs.loc file """ import optparse, os, subprocess, shutil, sys, tempfile, threading @@ -45,36 +74,31 @@ def __main__(): #Parse Command Line parser = optparse.OptionParser() - parser.add_option( '', '--ref_name', dest='ref_name', help='' ) - parser.add_option( '', '--ref_source', dest='ref_source', help='' ) + parser.add_option( '', '--ref_name', dest='ref_name', help='The reference name to append to all output matches' ) + parser.add_option( '', '--ref_source', dest='ref_source', help='Whether the reference is cached or from the history' ) parser.add_option( '', '--ref_sequences', dest='ref_sequences', help='Number of sequences in the reference dataset' ) - parser.add_option( '', '--source_select', dest='source_select', help='' ) - parser.add_option( '', '--out_format', dest='out_format', help='' ) - parser.add_option( '', '--input1', dest='input1', help='' ) - parser.add_option( '', '--input2', dest='input2', help='' ) - parser.add_option( '', '--pre_set_options', dest='pre_set_options', help='' ) - parser.add_option( '', '--strand', dest='strand', help='' ) - parser.add_option( '', '--seed', dest='seed', help='' ) - parser.add_option( '', '--transition', dest='transition', help='' ) - parser.add_option( '', '--gfextend', dest='gfextend', help='' ) - parser.add_option( '', '--nogfextend', dest='nogfextend', help='' ) - parser.add_option( '', '--chain', dest='chain', help='' ) - parser.add_option( '', '--nochain', dest='nochain', help='' ) - parser.add_option( '', '--gapped', dest='gapped', help='' ) - parser.add_option( '', '--nogapped', dest='nogapped', help='' ) - parser.add_option( '', '--O', dest='O', help='' ) - parser.add_option( '', '--E', dest='E', help='' ) - parser.add_option( '', '--X', dest='X', help='' ) - parser.add_option( '', '--Y', dest='Y', help='' ) - parser.add_option( '', '--K', dest='K', help='' ) - parser.add_option( '', '--L', dest='L', help='' ) - parser.add_option( '', '--entropy', dest='entropy', help='' ) - parser.add_option( '', '--identity_min', dest='identity_min', help='' ) - parser.add_option( '', '--identity_max', dest='identity_max', help='' ) - parser.add_option( '', '--coverage', dest='coverage', help='' ) - parser.add_option( '', '--format', dest='format', help='' ) + parser.add_option( '', '--source_select', dest='source_select', help='Whether to used pre-set or cached reference file' ) + parser.add_option( '', '--input1', dest='input1', help='The name of the reference file if using history or reference base name if using cached' ) + parser.add_option( '', '--input2', dest='input2', help='The reads file to align' ) + parser.add_option( '', '--pre_set_options', dest='pre_set_options', help='Which of the pre set options to use, if using pre-sets' ) + parser.add_option( '', '--strand', dest='strand', help='Which strand of the read to search, if specifying all parameters' ) + parser.add_option( '', '--seed', dest='seed', help='Seeding settings, if specifying all parameters' ) + parser.add_option( '', '--transition', dest='transition', help='Number of transitions to allow in each seed hit, if specifying all parameters' ) + parser.add_option( '', '--gfextend', dest='gfextend', help='Whether to perform gap-free extension of seed hits to HSPs (high scoring segment pairs), if specifying all parameters' ) + parser.add_option( '', '--chain', dest='chain', help='Whether to perform chaining of HSPs, if specifying all parameters' ) + parser.add_option( '', '--O', dest='O', help='Gap opening penalty, if specifying all parameters' ) + parser.add_option( '', '--E', dest='E', help='Gap extension penalty, if specifying all parameters' ) + parser.add_option( '', '--X', dest='X', help='X-drop threshold, if specifying all parameters' ) + parser.add_option( '', '--Y', dest='Y', help='Y-drop threshold, if specifying all parameters' ) + parser.add_option( '', '--K', dest='K', help='Threshold for HSPs, if specifying all parameters' ) + parser.add_option( '', '--L', dest='L', help='Threshold for gapped alignments, if specifying all parameters' ) + parser.add_option( '', '--entropy', dest='entropy', help='Whether to involve entropy when filtering HSPs, if specifying all parameters' ) + parser.add_option( '', '--identity_min', dest='identity_min', help="Minimum identity (don't report matches under this identity)" ) + parser.add_option( '', '--identity_max', dest='identity_max', help="Maximum identity (don't report matches above this identity)" ) + parser.add_option( '', '--coverage', dest='coverage', help="The minimum coverage value (don't report matches covering less than this)" ) + parser.add_option( '', '--out_format', dest='format', help='The format of the output file (sam, diffs, or tabular (general))' ) parser.add_option( '', '--output', dest='output', help='The output file' ) - parser.add_option( '', '--num_threads', dest='num_threads', help='' ) + parser.add_option( '', '--num_threads', dest='num_threads', help='The number of threads to run' ) parser.add_option( '', '--lastzSeqsFileDir', dest='lastzSeqsFileDir', help='Directory of local lastz_seqs.loc file' ) ( options, args ) = parser.parse_args() @@ -88,9 +112,9 @@ set_options = '--%s' % options.pre_set_options # Prepare for user-specified options else: - set_options = '%s %s --%s --%s --%s --%s O=%s E=%s X=%s Y=%s K=%s L=%s %s' % \ - ( options.gfextend, options.chain, options.gapped, options.strand, - options.seed, options.transition, options.O, options.E, options.X, + set_options = '--%s --%s --gapped --%s --%s --%s O=%s E=%s X=%s Y=%s K=%s L=%s --%s' % \ + ( options.gfextend, options.chain, options.strand, options.seed, + options.transition, options.O, options.E, options.X, options.Y, options.K, options.L, options.entropy ) # Specify input2 and add [fullnames] modifier if output format is diffs if options.format == 'diffs': @@ -107,18 +131,21 @@ if options.ref_source == 'history': # Reference is a fasta dataset from the history, so split job across number of # sequences in the dataset - try: - error_msg = "The reference dataset is missing metadata, click the pencil icon in the history item and 'auto-detect' the metadata attributes." - ref_sequences = int( options.ref_sequences ) - if ref_sequences < 1: - stop_err( error_msg ) - except: - stop_err( error_msg ) - for seq in range( ref_sequences ): +# try: +# error_msg = "The reference dataset is missing metadata, click the pencil icon in the history item and 'auto-detect' the metadata attributes." +# ref_sequences = int( options.ref_sequences ) +# if ref_sequences < 1: +# stop_err( error_msg ) +# except: +# stop_err( error_msg ) + # Currently set up to work only for a fasta file with a single sequence + for seq in range(1): +# for seq in range( ref_sequences ): command = 'lastz %s%s %s %s --ambiguousn --nolaj --identity=%s..%s --coverage=%s --format=%s%s >> %s' % \ ( ref_name, options.input1, input2, set_options, options.identity_min, options.identity_max, options.coverage, format, tabular_fields, options.output ) commands.append( command ) + print command else: # Reference is a locally cached 2bit file, split job across number of chroms in 2bit file tbf = TwoBitFile( open( options.input1, 'r' ) ) @@ -127,7 +154,6 @@ ( ref_name, options.input1, chrom, input2, set_options, options.identity_min, options.identity_max, options.coverage, format, tabular_fields, options.output ) commands.append( command ) - tbf.close() job_runner = LastzJobRunner( int( options.num_threads ), commands ) if __name__=="__main__": __main__() diff -r efdb6fb5de34 -r 341d7c1c5c9a tools/sr_mapping/lastz_wrapper.xml --- a/tools/sr_mapping/lastz_wrapper.xml Fri Nov 27 10:53:50 2009 -0500 +++ b/tools/sr_mapping/lastz_wrapper.xml Mon Nov 30 09:05:28 2009 -0500 @@ -6,16 +6,18 @@ #else: --ref_name="None" #end if ---ref_source=$source.ref_source --source_select=$params.source_select --out_format=$out_format.value --input1=$source.input1 --input2=$input2 +--ref_source=$source.ref_source --source_select=$params.source_select --out_format=$out_format.value --input2=$input2 #if $source.ref_source=="history": +--input1=$source.input1 --ref_sequences=$input1.metadata.sequences #else: +--input1=$source.input1_2bit --ref_sequences="None" #end if #if $params.source_select=="pre_set": ---pre_set_options=${params.pre_set_options} --strand="None" --seed="None" --transition="None" --O="None" --E="None" --X="None" --Y="None" --K="None" --L="None" --entropy="None" +--pre_set_options=${params.pre_set_options} --strand="None" --seed="None" --gfextend="None" --chain="None" --transition="None" --O="None" --E="None" --X="None" --Y="None" --K="None" --L="None" --entropy="None" #else: ---pre_set_options="None" --strand=$params.strand --seed=$params.seed --transition=$params.transition --O=$params.O --E=$params.E --X=$params.X --Y=$params.Y --K=$params.K --L=$params.L --entropy=$params.entropy +--pre_set_options="None" --strand=$params.strand --seed=$params.seed --gfextend=$params.gfextend --chain=$params.chain --transition=$params.transition --O=$params.O --E=$params.E --X=$params.X --Y=$params.Y --K=$params.K --L=$params.L --entropy=$params.entropy #end if --identity_min=$min_ident --identity_max=$max_ident --coverage=$min_cvrg --format=$out_format --output=$output1 --num_threads=$num_threads --lastzSeqsFileDir=${GALAXY_DATA_INDEX_DIR} </command> @@ -27,7 +29,7 @@ <option value="history">in your history</option> </param> <when value="cached"> - <param name="input1" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> + <param name="input1_2bit" type="select" label="Using reference genome" help="If your genome of interest is not listed, contact the Galaxy team"> <options from_file="lastz_seqs.loc"> <column name="value" index="1" /> <column name="name" index="0" /> @@ -39,7 +41,7 @@ </when> </conditional> <param name="out_format" type="select" label="Output format"> - <option value="sam">SAM</option> + <option value="sam-">SAM</option> <option value="diffs">Polymorphisms</option> <option value="tabular">Tabular</option> </param> @@ -61,29 +63,28 @@ </when> <when value="full"> <param name="strand" type="select" label="Which strand to search?"> - <option value="--strand=both">Both</option> - <option value="--strand=plus">Search forward strand only (the one in the reference)</option> - <option value="--strand=minus">Search the reverse complement strand only (opposite of the reference)</option> + <option value="strand=both">Both</option> + <option value="strand=plus">Search forward strand only (the one in the reference)</option> + <option value="strand=minus">Search the reverse complement strand only (opposite of the reference)</option> </param> <param name="seed" type="select" label="Select seeding settings" help="allows you set word size and number of mismatches"> - <option value="--seed=12of19">Seed hits require a 19 bp word with matches in 12 specific positions</option> - <option value="--seed=14of22">Seed hits require a 22 bp word with matches in 14 specific positions</option> + <option value="seed=12of19">Seed hits require a 19 bp word with matches in 12 specific positions</option> + <option value="seed=14of22">Seed hits require a 22 bp word with matches in 14 specific positions</option> </param> <param name="transition" type="select" label="Select transition settings" help="affects the number of allowed transition substitutions"> - <option value="--transition">Allow one transition in each seed hit</option> - <option value="--transition2">Allow two transitions in a seed hit </option> - <option value="--notransition">Don't allow any transitions in seed hits</option> + <option value="transition">Allow one transition in each seed hit</option> + <option value="transition2">Allow two transitions in a seed hit </option> + <option value="notransition">Don't allow any transitions in seed hits</option> </param> - <param name="gfextend" type="boolean" truevalue="--gfextend" falsevalue="--nogfextend" label="Perform gap-free extension of seed hits to HSPs (high scoring segment pairs)?" /> - <param name="chain" type="boolean" truevalue="--chain" falsevalue="--nochain" label="Perform chaining of HSPs?" /> - <param name="gapped" type="boolean" truevalue="--gapped" falsevalue="--nogapped" label="Perform gapped alignment (instead of gap-free)?" /> + <param name="gfextend" type="boolean" truevalue="gfextend" falsevalue="nogfextend" label="Perform gap-free extension of seed hits to HSPs (high scoring segment pairs)?" /> + <param name="chain" type="boolean" truevalue="chain" falsevalue="nochain" label="Perform chaining of HSPs?" /> <param name="O" type="integer" size="5" value="400" label="Gap opening penalty"/> <param name="E" type="integer" size="5" value="30" label="Gap extension penalty"/> <param name="X" type="integer" size="5" value="910" label="X-drop threshold"/> <param name="Y" type="integer" size="5" value="9370" label="Y-drop threshold"/> <param name="K" type="integer" size="5" value="3000" label="Set the threshold for HSPs (ungapped extensions scoring lower are discarded)"/> <param name="L" type="integer" size="5" value="3000" label="Set the threshold for gapped alignments (gapped extensions scoring lower are discarded)"/> - <param name="entropy" type="boolean" truevalue="--entropy" falsevalue="--noentropy" label="Involve entropy when filtering HSPs?" /> + <param name="entropy" type="boolean" truevalue="entropy" falsevalue="noentropy" label="Involve entropy when filtering HSPs?" /> </when> </conditional> <conditional name="seq_name"> @@ -119,7 +120,7 @@ <param name="input2" value="b1.fasta" ftype="fasta" /> <param name="ref_source" value="history" /> <param name="input1" value="phiX.fasta" ftype="fasta" /> - <param name="out_format" value="sam" /> + <param name="out_format" value="sam-" /> <param name="source_select" value="pre_set" /> <param name="pre_set_options" value="yasra95short" /> <param name="how_to_name" value="No" /> @@ -129,7 +130,6 @@ <param name="num_threads" value="4" /> <output name="output1" file="lastz_wrapper_out1.sam" /> </test> - <!-- <test> <param name="input2" value="b1.fasta" ftype="fasta" /> <param name="ref_source" value="cached" /> @@ -141,7 +141,6 @@ <param name="transition" value="transition" /> <param name="gfextend" value="true" /> <param name="chain" value="true" /> - <param name="gapped" value="gapped" /> <param name="O" value="400" /> <param name="E" value="30" /> <param name="X" value="910" /> @@ -157,7 +156,6 @@ <param name="num_threads" value="4" /> <output name="output1" file="lastz_wrapper_out2.tabular" /> </test> - --> <test> <param name="input2" value="b1.fasta" ftype="fasta" /> <param name="ref_source" value="history" /> @@ -171,7 +169,7 @@ <param name="min_cvrg" value="0" /> <param name="num_threads" value="4" /> <output name="output1" file="lastz_wrapper_out3.tabular" /> - </test> + </test> </tests> <help>