Galaxy error running NCBI BLAST: blastx
Hello, Can any one please tell me what could be causing this error message when I try to submit a blast job to Galaxy? ---- An error occurred running this job:BLAST Database error: No alias or index file found for protein database [/9720/genome_references/ncbi/nr-protein-db/nr-newstyle/nr] in search path [/9720/galaxyprod/galaxy-dist/database/job_working_directory/1454::] Return error code 2 from command:blast:blastx. ---- Thank you.
On Tue, Sep 11, 2012 at 3:11 PM, <kauerbach@comcast.net> wrote:
Hello,
Can any one please tell me what could be causing this error message when I try to submit a blast job to Galaxy?
An error occurred running this job:BLAST Database error: No alias or index file found for protein database [/9720/genome_references/ncbi/nr-protein-db/nr-newstyle/nr] in search path [/9720/galaxyprod/galaxy-dist/database/job_working_directory/1454::] Return error code 2 from command:blast:blastx.
That means that BLAST+ didn't find the NR database files. What does this give at the command line? ls /9720/genome_references/ncbi/nr-protein-db/nr-newstyle/nr* You should have a lot of files including nr.pal, nr.00.phd, ..,. nr.07.psq Perhaps you've got the wrong BLAST database path setup in the blastdb_p.loc file? Peter
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Peter Cock