It sounds like you're trying to determine the best adapter trimming approach for your Illumina Nextera 500 reads in Galaxy. Generally, if the Trimmomatic program includes a Nextera paired-end adapter cleaning option, it should be optimized for common Nextera adapters. However, if you're working specifically with Nextera 500, it’s worth checking whether there are any significant differences in adapter sequences. Running a test with both options and comparing results might help. On a different note, if you're looking for reliable junk removal services, you can check out <a href="https://junkbegoneusa.com/">https://junkbegoneusa.com/</a>.