Dear sir, I am using Australia Galaxy for assembling transcriptome lib. For cleaning of Illumina nextera 500 reads, I am using a trimmomatic program. This program contains nextera paired end adapter cleaning option. Now I am confused whether I should use galaxy nextera adapter or use specific nextera 500 adapter for cleaning. Kindly provide your suggestion. -- Rahul G. Shelke PhD Scholar Department of Biosciences and Bioengineering. Indian Institute Of Technology. Guwahati,Assam, India.781039 +91-9954919129 Skype ID:- rahulshelke2