Dear David:
thank you very much for helping me!
I have download the IGV and I do find the IGVtools, however, I am not sure which
tool I should use for generate a tdf file, the tile function will generate a tdf
file, but the input file format does not include bam or sam file, instead it
need wig file. But I have no wig file to put in. So I am wondering whether you
need to use other tool first. I really appreciate your help! Thank you very
much!
Best
Ying
Quoting David Matthews <
D.A.Matthews@bristol.ac.uk>:
Hi,
You can get an equivalent visualisation from the IGV viewer by the Broad Institute - its under IGV tools and generates a tdf file from bam or sam files. This also gives a quick and easy way of looking at depth at any particular site and is very accessible.
Cheers
David
On 21 Feb 2011, at 21:44, Jeremy Goecks wrote:
Hi all,
Ann is correct - Tophat does not produce .wig files when run anymore. However, it's fairly easy to use Galaxy to make a wiggle-like coverage file from a BAM file:
(a) run the pileup tool on your BAM to create a pileup file;
(b) cut columns 1 and 4 to get your coverage file.
A final note: it's often difficult to visualize coverage files because they're so large. You might be better off visualizing the BAM file and using the coverage file for statistics.
Best,
J.
Hello,
I think I know the answer (sort of) to this question.
This may be because newer versions of tophat stopped running the "wiggles"
program, which is still part of the tophat distribution and is the program
that makes the "coverage.wig" file.
A later version of tophat might bring this back, however - there's a note to
this effect in the tophat python code.
So if you can run wiggles, you can make the "coverage.wig" file on your own.
A student here at UNC Charlotte (Adam Baxter) made a few changes to the
"wiggles" source code that would allow you to use it with samtools to make a
"coverage.wig" file from the "accepted_hits.bam" file that TopHat creates.
If you (or anyone else) would like a copy, please email Adam, who is cc'ed
on this email.
We would be happy to help add it to Galaxy if this would be of interest to
you or other Galaxy users.
If there is any way we can be of assistance, please let us know!
Very best wishes,
Ann Loraine
On 2/21/11 3:39 PM, "Ying Zhang" <ying.zhang.yz323@yale.edu> wrote:
Hi:
I am using tophat in galaxy to analyze my paired-end RNA-seq data and find out
that after the tophat analysis, we can not get the wig file from it anymore
which is used to be able to. Do you have any idea of how to still be able to
get the wig file after tophat analysis? Thanks a lot!
Best
Ying Zhang, M.D., Ph.D.
Postdoctoral Associate
Department of Genetics,
Yale University School of Medicine
300 Cedar Street,S320
New Haven, CT 06519
Tel: (203)737-2616
Fax: (203)737-2286
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--
Ann Loraine
Associate Professor
Dept. of Bioinformatics and Genomics, UNCC
North Carolina Research Campus
600 Laureate Way
Kannapolis, NC 28081
704-250-5750
www.transvar.org
_______________________________________________
The Galaxy User list should be used for the discussion
of Galaxy analysis and other features on the public
server at usegalaxy.org. For discussion of local Galaxy
instances and the Galaxy source code, please use the
Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other
Galaxy lists, please use the interface at:
http://lists.bx.psu.edu/
_______________________________________________
The Galaxy User list should be used for the discussion
of Galaxy analysis and other features on the public
server at usegalaxy.org. For discussion of local Galaxy
instances and the Galaxy source code, please use the
Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other
Galaxy lists, please use the interface at:
http://lists.bx.psu.edu/
_______________________________________________
The Galaxy User list should be used for the discussion
of Galaxy analysis and other features on the public
server at usegalaxy.org. For discussion of local Galaxy
instances and the Galaxy source code, please use the
Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other
Galaxy lists, please use the interface at:
http://lists.bx.psu.edu/
Ying Zhang, M.D., Ph.D.
Postdoctoral Associate
Department of Genetics,
Yale University School of Medicine
300 Cedar Street,S320
New Haven, CT 06519
Tel: (203)737-2616
Fax: (203)737-2286