Pardon me, I see there is only one that says "tab-delimited" file. That is a tough one to decode. It almost looks like GTF already, but not quite. If we take it as a simple file of annotations on the genome, without structure such as exons, introns, and merely rework the columns to turn it into a bed file. Extract columns in this order: 4,5,6,2,7 to get a bed file with the accession identities: awk -F'\t' '{printf "%s\t%d\t%d\t%s\t%s\n", $4,$5,$6,$2,$7}' 59919.tab > 59919.bed It would take some time to figure out how to convert this file to something useful since I am not familiar with the format. I can't see immediately how to use it properly. --Hiram ----- Original Message ----- From: "Noa Sher" <noa.sher@gmail.com> To: "Hiram Clawson" <hiram@soe.ucsc.edu> Cc: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 11:10:06 AM Subject: Re: [galaxy-user] there was a wrong link in my previous mail - gtf file issues Hi Hiram, I was trying to work with the tab delineated file (using the link under export genomic data). Thanks noa On 04/12/2011 20:29, Hiram Clawson wrote: Good Morning Noa: Which one of the files at microbesonline are you trying to work with ? --Hiram ----- Original Message ----- From: "Noa Sher" <noa.sher@gmail.com> To: galaxy-user@lists.bx.psu.edu Sent: Sunday, December 4, 2011 1:38:35 AM Subject: [galaxy-user] there was a wrong link in my previous mail - gtf file issues The correct link: http://www.microbesonline.org/cgi-bin/genomeInfo.cgi?tId=59919