Dear Sir/Madam,

I am a PhD student working on RNA-Seq as my dissertation. As a matter of fact I want to do analyses of gene expressions for the sequences that I have and for that I have tried to use Galaxy tools as it is one of the best platforms in order to do analysis for RNA-Seq as well as others. I did all about installation of Galaxy on my Mac OS 10.7 Lion but when I run the commands I do not get any result from running any type of commands. And when I do run.sh I will have nothing done. As a sample I send you the command I ran on my system as follow

iMac-de-Biologia:galaxy-dist LabBioMol$ ./run.sh
Traceback (most recent call last):
  File "./scripts/paster.py", line 34, in <module>
    command.run()
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteScript-1.7.3-py2.7.egg/paste/script/command.py", line 84, in run
    invoke(command, command_name, options, args[1:])
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteScript-1.7.3-py2.7.egg/paste/script/command.py", line 123, in invoke
    exit_code = runner.run(args)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteScript-1.7.3-py2.7.egg/paste/script/command.py", line 218, in run
    result = self.command()
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteScript-1.7.3-py2.7.egg/paste/script/serve.py", line 276, in command
    relative_to=base, global_conf=vars)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteScript-1.7.3-py2.7.egg/paste/script/serve.py", line 313, in loadapp
    **kw)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 204, in loadapp
    return loadobj(APP, uri, name=name, **kw)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 224, in loadobj
    global_conf=global_conf)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 248, in loadcontext
    global_conf=global_conf)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 278, in _loadconfig
    return loader.get_context(object_type, name, global_conf)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 413, in get_context
    section)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 458, in _context_from_explicit
    value = import_string(found_expr)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/PasteDeploy-1.3.3-py2.7.egg/paste/deploy/loadwsgi.py", line 18, in import_string
    return pkg_resources.EntryPoint.parse("x="+s).load(False)
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/lib/pkg_resources.py", line 1954, in load
    entry = __import__(self.module_name, globals(),globals(), ['__name__'])
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/lib/galaxy/web/__init__.py", line 5, in <module>
    from framework import expose, json, json_pretty, require_login, require_admin, url_for, error, form, FormBuilder, expose_api
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/lib/galaxy/web/framework/__init__.py", line 31, in <module>
    from babel.support import Translations
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/Babel-0.9.4-py2.7.egg/babel/support.py", line 29, in <module>
    from babel.dates import format_date, format_datetime, format_time, LC_TIME
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/Babel-0.9.4-py2.7.egg/babel/dates.py", line 34, in <module>
    LC_TIME = default_locale('LC_TIME')
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/Babel-0.9.4-py2.7.egg/babel/core.py", line 642, in default_locale
    return '_'.join(filter(None, parse_locale(locale)))
  File "/Users/LabBioMol/galaxy-python/galaxy-dist/eggs/Babel-0.9.4-py2.7.egg/babel/core.py", line 763, in parse_locale
    raise ValueError('expected only letters, got %r' % lang)
ValueError: expected only letters, got 'utf-8'
iMac-de-Biologia:galaxy-dist LabBioMol$ 


Now I would appreciate if you could advise me what is the problem and how to solve it.

Thanks for your attention in advance.

Best regards

SMJ
--
SMJ

Seyed Mehdi Jazayeri