22 Jun
2011
22 Jun
'11
3:15 p.m.
Hi I have a few questions 1. I am trying to use Bowtie to map my data using a reference genome. the genome is available on Galaxy (Escherichia coli DH10B (20079)). Is there a way that I can download the file in fasta format to use as a reference in Tablet? I can import the SAM output into Tablet if I do not give it a reference, but I would like to see it with the reference 2. I have used the Sam to Bam converter and downloaded the file to my computer then tried to import it into Tablet, but it gives an error. Does anyone have any advice on doing this? 3. Does Galaxy have any tools for doing a blast search against a reference genome or database? Any help/instructions that you point me to is greatly appreciated. Jo