Hello Ateeq, Custom reference genomes are loaded in FASTA format. Help for preparing the data is in our wiki: http://wiki.g2.bx.psu.edu/Support#Custom_reference_genome http://wiki.g2.bx.psu.edu/Learn/CustomGenomes Best, Jen Galaxy team On 6/14/12 9:15 AM, Ateequr Rehman wrote:
Dear All
I am analysing bacterial RNA seq (illumina). as my reference genome is not in galaxy. so i need to downlaoded the reference genome from NCBI and upload to my history
Should i use reference genome with gene features or single fasta file with out any annotation information. even after download should i need to modify or it should be used as it is.
Thanks in advance Ateeq
___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other Galaxy lists, please use the interface at:
-- Jennifer Jackson http://galaxyproject.org