Hi Jen, I have a related question. If Illumina 1.9 is already in Sanger format, is it still necessary to groom the FASTQ files for TopHat? Would it be enough to directly change the data type to Sanger without grooming? Thanks, Carlos On Wed, Feb 29, 2012 at 10:57 AM, Jennifer Jackson <jen@bx.psu.edu> wrote:
Hello,
The input quality score type should be set as "Sanger" for your data.
Thanks!
Jen Galaxy team
On 2/29/12 7:39 AM, Ateequr Rehman wrote:
Dear Glaxy users and admin
I ran my sequence data on FASTQC tool, output says it is Encoding Sanger / Illumina 1.9
now i want to groom my file, but groomer does not have option for 1.9 in "Input FASTQ quality scores type"
any idea which option i should select to grroom my file,
later i want to run Bowtie or Tophat,
Thanks **
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-- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org/wiki/Support ___________________________________________________________ The Galaxy User list should be used for the discussion of Galaxy analysis and other features on the public server at usegalaxy.org. Please keep all replies on the list by using "reply all" in your mail client. For discussion of local Galaxy instances and the Galaxy source code, please use the Galaxy Development list:
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