Hi Philipp, Sorry for the delay in reply, the question is a bit confusing since the conversion tools allow for the adapter base to be specified (and the default is a G). I am not sure we are talking about the same tool, so to clear things up, would you please send a shared history link and any other details that you think will help, and then I'll try to provide some more feedback? Including opening an enhancement request ticket if that seems appropriate after we discuss the data & tools. To share, use "Options -> Share or Publish", generate the share link, copy that, and email it directly back to me (not the entire list). Make certain that all input are present and any attempted tool runs, successful or failure are present and undeleted. Please note the exact tools that you want to use, including the settings you think are the best fit, and what you think is missing, the more detail the better. Thank you and I will watch for your reply, Best, Jen Galaxy team On 2/2/12 9:41 AM, Philipp.Berninger@unibas.ch wrote:
Hi,
I have a problem converting paired reads with paired end reads from ABi, in the color code the reads started with a G and afterwards with numbers instead of T02102103... , so I guess the program assumes the T as hardcoded instead of using the G as last base
best
Philipp
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