11 Apr
2011
11 Apr
'11
3:43 p.m.
Hi, I used the Megablast function (in the NGS: Mapping\ROCHE-454\) to analyze my FASTA sequences against nt database and it worked fine for me. However, it generated 56,804 hits although my query has only 1000 sequences. I am wondering is there any way to suppress the number of reported alignments to just one best hit per sequence? (In the local BLAST there are parameters such as -K1 -v 1 -b 1 to do so, but I can't find similar options in Galaxy). Many thanks! Sam