HI,

I am very new to genomic data analysis and I need to get some upstream and downstream of some chromosome regions of the pig genome. I have about 70 blat hits of a query of ca 100aa. I need to get 7000 nucleotides both upstream and downstream of this 100aa region.

I have tried to use Get flanks to get the "new" coordinates... bus instead of generating coordinates which would correspond to about 14000 nucleotides, it generates one coordinate for the upstream region and them another one for the downstream region.

Is there a way of doing what I need using Galaxy? 

I would appreciate any help!

Thanks a lot!

All the best,

Fabricia.