Hello Jiwen, No, you do not need to join the files for the quality processing. Hopefully this helps! Best, Jen Galaxy team On 4/9/12 9:14 AM, 杨继文 wrote:
Hi all, I have paired end RNA-Seq reads ( Illumina Hiseq 2000) in seperate files. Before mapping, I need to trim the reads.
My questions is : Do I have to join pair end reads before timming, and then split again for Tophat???
Lookiong forward to your answers.
Thanks
Jiwen
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