Hello,

The Megablast htgs, nt, and wgs databases are in the process of being updated to the latest NCBI releases and are expected to be available by tomorrow morning (possibly sooner).

Should you wish to continue your analysis using the prior versions, these are available through our rsync server for use in a local production or cloud Galaxy.

https://wiki.galaxyproject.org/Admin/UseGalaxyRsync
http://getgalaxy.org
http://usegalaxy.org/cloud

Also posted to Galaxy Biostar: https://biostar.usegalaxy.org/p/7335/#7340

Best,
Jen
Galaxy team

On 4/27/14 4:25 AM, Scott W. Tighe wrote:

Jennifer

I am megablasting a simple 500,000 line dataset that is certainly in galaxy fasta.

For a week i have been seeing numerous errors. So i have reprocesed the data multiple times.

The error message is "could not find specified database directory"

Is there an alternative approach? I did try ncbi directly and that failed too. They say they have a database issue

Scott Tighe

Core Laboratory Research Staff
Advanced Genome Technologies Core
Deep Sequencing (MPS) Facility
Vermont Cancer Center
149 Beaumont Ave
University of Vermont HSRF 303
Burlington Vermont USA 05045
802-656-AGTC
802-999-6666 (cell)

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