11 Oct
2013
11 Oct
'13
11:51 a.m.
Hi, I'm new to Galaxy and am trying to view several miRNA datasets as a differential expression. The pipeline I'm using is Bowtie for Illumina (paired-end run) > SAM-to-BAM > ? > xls. The references I used with Bowtie are a mature miRNA fasta and a piRNA fasta and the reads are 30nt in length. So, my questions are: Is this the proper pipeline? How do I go about converting the BAM into a xls file viewable in Excel? Thanks!