I want to have list of genes from UCSC browser or known genes.
Hello Kanwar,
On 7/20/12 3:31 PM, shamsher jagat wrote:
The "Region:" options are:I am interested in getting regions flanking TSS, I am using Glaxaxy and
have downloaded TSS sites using
this post steps
https://lists.soe.ucsc.edu/pipermail/genome/2011-June/026175.html
Now what I would like to do is to get 5000 bp upstream an
downstream using flank tool in galaxy, but i realize it only gave me
option for gene start or whole gene.
1 - around start - meaning interval start coordinate
2 - around end - meaning interval end coordinate
3 - whole gene - meaning entire intervals
Pick option #1.The "Location of the flanking region/s:" options are:
Is it possible to extract 5000 bp upstream and downstream regions across
tss start site .
4 - Upstream
5 - Downstream
6 - Both
Pick option #6 with "Length of the flanking region(s):" set to 5000.Do you want to identify/label known genes or discover novel genes? This part of your question is not clear. Could you explain in more detail the end goal?
Once I have that then I want to find non overlaping
genes in my regions from chipseq data.
It is likely some for of the tool "Operate on Genomic Intervals - > Merge will do what you want", but it is difficult to recommend the correct option.
Going forward, sending question to a single public list, as Brooke also suggests, is best. It is generally considered a good idea to not post to two or more, at the same time, with the same email to start threads.
Thanks!
jen
Galaxy team
Thanks
Kanwar
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