2 Apr
2012
2 Apr
'12
3:32 p.m.
Hi, I have NGS results of DNA enriched for exons with an AllExon kit (Agilent). I have a bed file with the list of targeted sequences. I want to use GATK DepthOfCoverage to compare the results to the bed file and to get all the targets that were covered by <n reads. How can I do that with Galaxy on the web? or in Amazon? My question has 2 parts: 1. How can I specify the target intervals in Galaxy on the web? (the "-L" command in Unix commandline) 2. How can I ask the coverage for single bases instead of statistics? Thanks, Lilach