I have used Trinity on a local installation here at Bristol University. The main reason its not on Galaxy main is because its very very memory intensive (we run it on nodes with 256GB RAM). So you really need access to a big machine to run it. Having said all that the output is astoundingly good so it's worth the time and effort to get a run going if you can.

On 21 Dec 2011, at 13:36, Jane Song wrote:

Dear Galaxy Expert,

I would like to use Galaxy to de-novo assembly single-end read illumina data (140bp) for plant transcriptomes (without reference). I remember early emails mention trinity in Galaxy. But I could not see at Galaxy web http://main.g2.bx.psu.edu/root
. Maybe it is installed in Amarzon EC2? Other suggestions in de-novo assembly plant transcriptomes without reference.

Many thanks and look forward to hearing back from you,
Jane

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