NGS: SAM Tools

 

I have generated a simple 6 column pileup from my BAM file but when I try to use the Filter Pileup tool it doesn’t recognise my pileup file, I receive the message “History does not include a dataset of the required format / build”. Build is hg19. Curiously in the same history I have ran the tool through a workflow without a hitch.

 

Any advice to successfully filter my pileup would be gratefully received.

 

Many thanks,

Andy