Dear All,<div><br></div><div><span style="background-color:rgb(255,255,255)">I have been successful by using the online tool to align Illumina pair end reads , each direction 5GB, and also generated a pileup of <span style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:14px">680,000,000 lines,</span></span></div>
<div><span style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:14px;background-color:rgb(255,255,255)">but the filtering of the pileup always fails, it runs for several hours and I get an empty file back. I tried different options and different pileups, always the same.</span></div>
<div><span style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:14px;background-color:rgb(255,255,255)">Could somebody please help or what is the trick?</span></div>
<div><span style="color:rgb(48,48,48);font-family:&#39;Lucida Grande&#39;,verdana,arial,helvetica,sans-serif;font-size:12px;line-height:14px;background-color:rgb(255,255,255)"><br></span></div><div><font color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif"><span style="font-size:12px;line-height:14px">Thank you</span></font></div>
<div><font color="#303030" face="&#39;Lucida Grande&#39;, verdana, arial, helvetica, sans-serif"><span style="font-size:12px;line-height:14px">Sebahattin</span></font></div>