Im trying to map RNA-seq data to a reference genome in Galaxy (Main instance) using both Bowtie 1 and Bowtie 2. Im currently using publicly available data (
http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE28755)
which is not available in FASTQ format - only the raw sequences with the read count. Is there a way to set the input data for bowtie to raw, as is possible using the terminal? Or is there a way to convert a raw sequence to FASTQ (not sure if this would work,
but it might be possible if I assigned accession numbers to each sequence and made all the quality scores identical)?
Thanks very much