HI,
I am new to the RNA-seq, and the only
available sources for me to do analysis is the Galaxy server. I want find out
SNP and point mutations in RNA-Seq data using Galaxy (I do not know if anyone
using RNA-seq data to find point mutations, because there is whole Genome
sequencing for reporting mutations and SNPs). I have been searching in the forum
for a step-by-step protocols for doing it, but could not find it.
I have one
normal sample and two cancer samples, a TopHat produced "accepted Hits.bam" file
for each one.
I want to find out SNP and point mutations in the cancer
samples, so How do I go from here? Can anyone show me how to do it in Galaxy
main server?
Thanks!
Wei